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Gene Expression Data
Assay Details
Assay
Reference: J:82208 Greferath U, et al., Developmental expression of EphA4-tyrosine kinase receptor in the mouse brain and spinal cord. Mech Dev. 2002 Dec;119 Suppl 1:S231-8
Assay type: Immunohistochemistry
MGI Accession ID: MGI:5494449
Gene symbol: Epha4
Gene name: Eph receptor A4
Antibody: Sek1I antibody
Detection system: Secondary antibody coupled to Alexa Fluor
Results
Specimen 1A: embryonic day 11.0 (more )
Structure Level Pattern Image Note
TS18: central nervous system Present Widespread 1A
TS18: diencephalon Present 1A
TS18: face Present 1A
TS18: future spinal cord Present 1A
TS18: telencephalon Present 1A
TS18: hindbrain Present 1A Strongest in the pontine flexure.
TS18: skeleton Present Regionally restricted 1A Expression is in the cranium.

Specimen 1B: embryonic day 13.5 (more )
Structure Level Pattern Image Note
TS21: central nervous system Present Widespread 1B
TS21: cranium Present 1B
TS21: diencephalon Present 1B
TS21: face Present 1B
TS21: hindbrain Present 1B
TS21: spinal cord Present 1B
TS21: telencephalon Present 1B
TS21: corpus striatum Strong 1B
TS21: forebrain Present Regionally restricted 1B Expression is in the hippocampus.

Specimen 1C: embryonic day 15.5 (more )
Structure Level Pattern Image Note
TS23: telencephalon Present 1C
TS23: corpus striatum Strong 1C
TS23: cerebellum Present Regionally restricted 1C Expression is in a dorsal patch.

Specimen 1D: embryonic day 17.5 (more )
Structure Level Pattern Image Note
TS25: hindbrain Present 1D
TS25: telencephalon Present 1D
TS25: corpus striatum Strong 1D

Specimen 1E: postnatal day 2 (more )
Structure Level Pattern Image Note
TS27: telencephalon Present Regionally restricted 1E Expression is in structures derived from the telencephalon, including a major tract and the internal capsule.
TS27: cerebellum Present 1E
TS27: dentate gyrus Present 1E
TS27: hippocampus CA3 Present 1E
TS27: corpus striatum Strong 1E
TS27: hippocampus Present Regionally restricted 1E Expression is in the hippocampal commissure.

Specimen 1F: postnatal day 4 (more )
Structure Level Pattern Image Note
TS28: telencephalon Present Regionally restricted 1F Expression is in structures derived from the telencephalon, including a major tract and the internal capsule.
TS28: cerebellum Present 1F
TS28: corpus striatum Strong 1F

Specimen 2A: embryonic day 11.0 (more )
Structure Level Pattern Image Note
TS18: telencephalon Present 2A Strongest in the outermost pial area.

Specimen 2B: embryonic day 13.5 (more )
Structure Level Pattern Image Note
TS21: cerebral cortex ventricular layer Present 2B
TS21: cortical preplate Present 2B

Specimen 2C: embryonic day 15.5 (more )
Structure Level Pattern Image Note
TS23: cerebral cortex marginal layer Strong 2C
TS23: cerebral cortex ventricular layer Strong 2C
TS23: cerebral cortex intermediate zone Strong Regionally restricted 2C Expression is in a striated pattern.
TS23: cortical plate Present Regionally restricted 2C Expression is on radial processes.

Specimen 2D: postnatal day 0 (more )
Structure Level Pattern Image Note
TS27: cerebral hemisphere white matter Present 2D
TS27: cortical layer V Present 2D
TS27: cortical layer VI Present 2D

Specimen 2E: postnatal day 2 (more )
Structure Level Pattern Image Note
TS27: cortical layer III Strong Regionally restricted 2E Expression is in the medio-lateral region of the cortex.
TS27: cortical layer IV Strong Regionally restricted 2E Expression is in the medio-lateral region of the cortex.
TS27: cerebral internal capsule Present 2E
TS27: midbrain Present Regionally restricted 2E Expression is in the cerebral peduncle.

Specimen 2F: postnatal day 4 (more )
Structure Level Pattern Image Note
TS28: cortical layer III Strong Regionally restricted 2F Expression is in the medio-lateral region of the cortex.
TS28: cortical layer IV Strong Regionally restricted 2F Expression is in the medio-lateral region of the cortex.
TS28: cerebral internal capsule Present 2F
TS28: dentate gyrus Present 2F
TS28: hippocampus CA3 Present 2F
TS28: midbrain Present Regionally restricted 2F Expression is in the cerebral peduncle.
TS28: hippocampus Present Regionally restricted 2F Expression is in the hippocampal commissure.

Specimen 2G: postnatal day 6 (more )
Structure Level Pattern Image Note
TS28: corpus callosum Present Regionally restricted 2G In the caudal part expression is dorsal.
TS28: forebrain Present Regionally restricted 2G Expression is in axon tracts which emanate from either the cortex or thalamus.

Specimen 2H: postnatal day 6 (more )
Structure Level Pattern Image Note
TS28: corpus callosum Present Regionally restricted 2H In the caudal part expression is dorsal.
TS28: forebrain Present Regionally restricted 2H Expression is in axon tracts which emanate from either the cortex or thalamus.
TS28: hippocampus Present Regionally restricted 2H Expression is in the hippocampal commissure.
TS28: dentate gyrus Present 2H
TS28: hippocampus CA3 Present 2H

Specimen 2I,J,K: embryonic day 17.5 (more )
Note: (J,K) are higher magnifications of the boxed areas in (I).
Structure Level Pattern Image Note
TS25: forebrain Present Regionally restricted 2J , 2K , 2I Expression is in axon tracts.
TS25: anterior commissure Present 2K , 2I
TS25: corpus striatum Present Regionally restricted 2I , 2K In fiber bundles.

Specimen 2L: embryonic day 13.5 (more )
Structure Level Pattern Image Note
TS21: brain Present Regionally restricted 2L Expression is in thalamo-cortical projections.
TS21: forebrain Present Regionally restricted 2L Expression is in the hippocampus.
TS21: thalamus Present 2L

Specimen 2M,N: embryonic day 13.5 (more )
Note: (N) is a higher magnification of the boxed area in (M).
Structure Level Pattern Image Note
TS21: brain Present Regionally restricted 2M , 2N Expression is in thalamo-cortical projections.
TS21: thalamus Present 2M

Specimen 2O: embryonic day 15.5 (more )
Structure Level Pattern Image Note
TS23: neurohypophysis Strong 2O
TS23: neurohypophysis infundibulum Strong 2O
TS23: adenohypophysis Absent 2O

Specimen 3A: embryonic day 17.5 (more )
Structure Level Pattern Image Note
TS25: cerebellum Present Regionally restricted 3A Expression was on some, but not all, of the lobules and in the region of the nuclei.

Specimen 3B: postnatal day 0 (more )
Structure Level Pattern Image Note
TS27: cerebellum Purkinje cell layer Present 3B
TS27: deep cerebellar nucleus Present 3B

Specimen 3C: postnatal day 6 (more )
Structure Level Pattern Image Note
TS28: cerebellum Purkinje cell layer Present 3C
TS28: deep cerebellar nucleus Present 3C

Specimen 3D: embryonic day 15.5 (more )
Structure Level Pattern Image Note
TS23: cerebellum Present Regionally restricted 3D Expression is in the inferior cerebellar peduncle.
TS23: trigeminal nerve Present 3D

Specimen 3E: embryonic day 15.5 (more )
Structure Level Pattern Image Note
TS23: vibrissa Present 3E Expression is in the shaft and in a circular structure around it.
TS23: trigeminal nerve Present 3E

Specimen 3F,F': embryonic day 17.5 (more )
Note: Double labeled: red - Epha4; green - Nefm (assay MGI:5494625).
Structure Level Pattern Image Note
TS25: inner ear Present Regionally restricted 3F , 3F' Expression is in areas of future scala vestibuli and scala tympani.
TS25: cochlear ganglion Absent 3F' , 3F

Specimen 4A: embryonic day 11.0 (more )
Structure Level Pattern Image Note
TS18: future spinal cord Present 4A There is a slight increase in expression in the medio-lateral regions.
TS18: neural tube ventricular layer Strong 4A

Specimen 4B: embryonic day 13.5 (more )
Structure Level Pattern Image Note
TS21: basal columns Strong 4B
TS21: spinal cord Present Regionally restricted 4B Expression in dorsal areas is medial.

Specimen 4C,C': embryonic day 17.5 (more )
Structure Level Pattern Image Note
TS25: intermediate grey horn Present 4C , 4C'
TS25: ventral grey horn Present 4C , 4C'
TS25: spinal cord Present Regionally restricted 4C' , 4C Expression is strong in the dorsal funiculus and in the ventral funiculus as well as in the ventral and lateral white matter columns.

Specimen Table 1 E11: embryonic day 11.0 (more )
Structure Level Pattern Image Note
TS18: telencephalon Weak Regionally restricted Expression is in the frontal/parietal, and occipital cortex.
TS18: forebrain Weak Regionally restricted Expression is in the hippocampus.
TS18: diencephalon Weak Regionally restricted Expression is in the thalamus.
TS18: midbrain Weak Regionally restricted Expression is in the superior colliculus.
TS18: future spinal cord Weak

Specimen Table 1 E13.5: embryonic day 13.5 (more )
Structure Level Pattern Image Note
TS21: cerebral cortex Weak Regionally restricted Expression is in the frontal/parietal, occipital cortex, cingulate, subiculum, basal ganglia, internal capsule, and cerebral peduncle.
TS21: forebrain Present Regionally restricted Expression is in the hippocampus.
TS21: diencephalon Present Regionally restricted Expression is in the thalamo-cortical projection.
TS21: spinal cord Present
TS21: thalamus Present
TS21: eye Weak
TS21: hypothalamus Weak
TS21: neurohypophysis infundibulum Weak
TS21: midbrain Weak Regionally restricted Expression is in the superior colliculus.

Specimen Table 1 E15: embryonic day 15.0 (more )
Structure Level Pattern Image Note
TS23: telencephalon Strong Regionally restricted Expression is in the frontal/parietal, occipital cortex, cingulate, subiculum, basal ganglia, internal capsule, and cerebral peduncle.
TS23: caudate nucleus Strong
TS23: caudate-putamen Strong
TS23: cerebral cortex Strong
TS23: hippocampus Very strong Moderate expression in CA1-3, dentate gyrus, weaker in fimbria and the ventral hippocampal commissure.
TS23: cochlea Present
TS23: pons Present
TS23: spinal cord Present
TS23: thalamus Present
TS23: diencephalon Present Regionally restricted Expression is in the thalamo-cortical projection.
TS23: hindbrain Present Regionally restricted Expression is in the facial nucleus, ventral lateral lemniscus, olivary region, principle sensory trigeminal nucleus, spinal trigeminal nucleus, and lateral superior olive.
TS23: basal ganglia Weak Regionally restricted Expression is in the globus pallidus.
TS23: eye Weak
TS23: hypothalamus Weak
TS23: main olfactory bulb Weak
TS23: neurohypophysis infundibulum Weak
TS23: vibrissa Weak
TS23: midbrain Weak Regionally restricted Expression is in the superior colliculus, anterior pretectal nucleus, mediate geniculate nucleus, mammillary peduncle, red nucleus, and posterior commisure.
TS23: cerebellum Weak Regionally restricted Expression is in the cerebellum including the Purkinje cell layer, cerebellar nuclei, and the inferior cerebellar peduncle.

Specimen Table 1 E17.5: embryonic day 17.5 (more )
Structure Level Pattern Image Note
TS25: hippocampus Very strong Moderate in CA1-3, fimbria, and ventral hippocampal commissure.
TS25: cerebral cortex Strong Regionally restricted Expression is in the frontal/parietal, occipital cortex, cingulate, subiculum, corpus callosum, internal capsule, and cerebral peduncle.
TS25: caudate nucleus Strong
TS25: caudate-putamen Strong
TS25: dentate gyrus Present
TS25: hypothalamus Present
TS25: neurohypophysis infundibulum Present
TS25: spinal cord Present
TS25: diencephalon Present Regionally restricted Expression is in the thalamo-cortical projection.
TS25: hindbrain Present Regionally restricted Expression is in the facial nucleus, ventral lateral lemniscus, olivary region, principle sensory trigeminal nucleus, spinal trigeminal nucleus, and lateral superior olive.
TS25: cerebellum Present Regionally restricted Expression is in the cerebellum including the Purkinje cell layer, cerebellar nuclei, and the inferior cerebellar peduncle.
TS25: telencephalon Weak Regionally restricted Expression is in the globus pallidus.
TS25: cochlea Weak
TS25: eye Weak
TS25: superior colliculus Weak
TS25: thalamus Weak
TS25: vibrissa Weak
TS25: midbrain Weak Regionally restricted Expression is in the anterior pretectal nucleus, mediate geniculate nucleus, mammillary peduncle, red nucleus, and posterior commisure.

Specimen Table 1 P0: postnatal day 0 (more )
Structure Level Pattern Image Note
TS27: caudate nucleus Present
TS27: cerebellum Present
TS27: cerebellum Purkinje cell layer Present
TS27: cerebral cortex Present
TS27: cerebral internal capsule Present
TS27: deep cerebellar nucleus Present
TS27: dentate gyrus Present
TS27: facial nucleus Present
TS27: fimbria hippocampus Present
TS27: frontal cortex Present
TS27: hippocampus CA1 Present
TS27: hippocampus CA2 Present
TS27: hippocampus CA3 Present
TS27: hypothalamus Present
TS27: main olfactory bulb Present
TS27: neurohypophysis Present
TS27: parietal cortex Present
TS27: pons Present
TS27: spinal cord Present
TS27: superior olivary nucleus Present
TS27: diencephalon Present Regionally restricted Expression is in the thalamo-cortical projection.
TS27: hippocampus Present Including the hippocampal commissure.
TS27: cerebellar peduncle Weak
TS27: cingulate cortex Weak
TS27: cochlea Weak
TS27: corpus callosum Weak
TS27: eye Weak
TS27: globus pallidus Weak
TS27: medial geniculate nucleus Weak
TS27: occipital cortex Weak
TS27: posterior commissure Weak
TS27: putamen Weak
TS27: red nucleus Weak
TS27: subiculum Weak
TS27: superior colliculus Weak
TS27: thalamus Weak
TS27: trigeminal V sensory nucleus Weak
TS27: trigeminal V spinal sensory nucleus Weak
TS27: vibrissa Weak
TS27: midbrain Weak Regionally restricted Anterior pretectal nucleus and mammillary peduncle, stonger in the cerebral peduncle.

Specimen Table 1 P2: postnatal day 2 (more )
Structure Level Pattern Image Note
TS27: caudate nucleus Present
TS27: cerebellum Present
TS27: cerebellum Purkinje cell layer Present
TS27: cerebral cortex Present
TS27: cerebral internal capsule Present
TS27: deep cerebellar nucleus Present
TS27: dentate gyrus Present
TS27: fimbria hippocampus Present
TS27: frontal cortex Present
TS27: hippocampus CA1 Present
TS27: hippocampus CA2 Present
TS27: hippocampus CA3 Present
TS27: parietal cortex Present
TS27: putamen Present
TS27: spinal cord Present
TS27: superior olivary nucleus Present
TS27: diencephalon Present Regionally restricted Expression is in the thalamo-cortical projection.
TS27: hippocampus Present Regionally restricted Including the hippocampal commissure.
TS27: cerebellar peduncle Weak
TS27: cingulate cortex Weak
TS27: corpus callosum Weak
TS27: facial nucleus Weak
TS27: globus pallidus Weak
TS27: hypothalamus Weak
TS27: main olfactory bulb Weak
TS27: medial geniculate nucleus Weak
TS27: neurohypophysis Weak
TS27: occipital cortex Weak
TS27: pons Weak
TS27: posterior commissure Weak
TS27: red nucleus Weak
TS27: subiculum Weak
TS27: superior colliculus Weak
TS27: thalamus Weak
TS27: trigeminal V sensory nucleus Weak
TS27: trigeminal V spinal sensory nucleus Weak
TS27: midbrain Weak Regionally restricted Anterior pretectal nucleus and mammillary peduncle, stonger in the cerebral peduncle.

Specimen Table 1 P4: postnatal day 4 (more )
Structure Level Pattern Image Note
TS28: caudate nucleus Present
TS28: cerebellum Present
TS28: cerebellum Purkinje cell layer Present
TS28: cerebral internal capsule Present
TS28: deep cerebellar nucleus Present
TS28: putamen Present
TS28: spinal cord Present
TS28: superior olivary nucleus Present
TS28: cerebellar peduncle Weak
TS28: cerebral cortex Weak
TS28: cingulate cortex Weak
TS28: corpus callosum Weak
TS28: dentate gyrus Weak
TS28: facial nucleus Weak
TS28: fimbria hippocampus Weak
TS28: frontal cortex Weak
TS28: globus pallidus Weak
TS28: hippocampus CA1 Weak
TS28: hippocampus CA2 Weak
TS28: hippocampus CA3 Weak
TS28: main olfactory bulb Weak
TS28: medial geniculate nucleus Weak
TS28: occipital cortex Weak
TS28: parietal cortex Weak
TS28: pons Weak
TS28: posterior commissure Weak
TS28: red nucleus Weak
TS28: subiculum Weak
TS28: superior colliculus Weak
TS28: thalamus Weak
TS28: trigeminal V sensory nucleus Weak
TS28: trigeminal V spinal sensory nucleus Weak
TS28: midbrain Weak Regionally restricted Anterior pretectal nucleus and mammillary peduncle, stonger in the cerebral peduncle.
TS28: hippocampus Weak Regionally restricted Including the hippocampal commissure.
TS28: diencephalon Weak Regionally restricted Expression is in the thalamo-cortical projection.
TS28: hypothalamus Absent
TS28: neurohypophysis Absent

Specimen Table 1 P6: postnatal day 6 (more )
Structure Level Pattern Image Note
TS28: cerebellum Present
TS28: cerebellum Purkinje cell layer Present
TS28: cerebral internal capsule Present
TS28: deep cerebellar nucleus Present
TS28: spinal cord Present
TS28: superior olivary nucleus Present
TS28: caudate nucleus Weak
TS28: cerebellar peduncle Weak
TS28: cerebral cortex Weak
TS28: corpus callosum Weak
TS28: dentate gyrus Weak
TS28: facial nucleus Weak
TS28: fimbria hippocampus Weak
TS28: frontal cortex Weak
TS28: globus pallidus Weak
TS28: hippocampus CA1 Weak
TS28: hippocampus CA2 Weak
TS28: hippocampus CA3 Weak
TS28: main olfactory bulb Weak
TS28: medial geniculate nucleus Weak
TS28: occipital cortex Weak
TS28: parietal cortex Weak
TS28: pons Weak
TS28: posterior commissure Weak
TS28: putamen Weak
TS28: red nucleus Weak
TS28: subiculum Weak
TS28: superior colliculus Weak
TS28: thalamus Weak
TS28: trigeminal V sensory nucleus Weak
TS28: trigeminal V spinal sensory nucleus Weak
TS28: midbrain Weak Anterior pretectal nucleus and mammillary peduncle, stonger in the cerebral peduncle.
TS28: hippocampus Weak Regionally restricted Including the hippocampal commissure.
TS28: diencephalon Weak Regionally restricted Expression is in the thalamo-cortical projection.
TS28: cingulate cortex Absent
TS28: hypothalamus Absent
TS28: neurohypophysis Absent


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last database update
03/25/2025
MGI 6.24
The Jackson Laboratory