Probe:	mSlit2 probe
Probe preparation:	labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS15: neural tube floor plate	Present		3E
TS15: neural tube roof plate	Present		3E
TS15: notochord	Present		3E

Structure	Level	Pattern	Image	Note
TS17: neural tube floor plate	Strong	Regionally restricted	3F	Strong expression in motor columns.
TS17: neural tube lateral wall	Strong	Regionally restricted	3F	Strong expression in motor columns.
TS17: neural tube roof plate	Strong	Regionally restricted	3F	Strong expression in motor columns.
TS17: notochord	Strong	Regionally restricted	3F	Strong expression in motor columns.

Structure	Level	Pattern	Image	Note
TS19: neural tube floor plate	Present		3G
TS19: neural tube roof plate	Present		3G
TS19: notochord	Present		3G
TS19: neural tube basal columns	Strong		3G	Strong expression in motor columns.

Structure	Level	Pattern	Image	Note
TS21: notochord	Present		3H
TS21: spinal cord floor plate	Present		3H
TS21: basal columns	Strong		3H	Strong expression in motor columns.
TS21: spinal cord lateral wall	Present	Regionally restricted	3H	Weak expression in commissural neurons.
TS21: spinal cord roof plate	Weak		3H

Structure	Level	Pattern	Image	Note
TS13: future hindbrain neural fold	Present	Regionally restricted	4E	Expression in dorsal neuroepithelium overlying hindbrain (neural fold).
TS13: future midbrain	Present	Regionally restricted	4E	Expression in ventral midbrain and tectum.
TS13: future prosencephalon	Present	Regionally restricted	4E	Expression in commissural plate.

Structure	Level	Pattern	Image	Note
TS13: future hindbrain neural fold	Present	Regionally restricted	4F	Expression in dorsal neuroepithelium overlying hindbrain (neural fold).
TS13: future midbrain	Present	Regionally restricted	4F	Expression in ventral midbrain and tectum.
TS13: branchial groove	Present	Regionally restricted	4F	Expression in clefts between first, second and third branchial arches.

Structure	Level	Pattern	Image	Note
TS14: future hindbrain	Present	Regionally restricted	4G	Expression in dorsal neuroepithelium overlying hindbrain.
TS14: future midbrain	Present	Regionally restricted	4G	Expression in ventral midbrain and tectum.
TS14: prosencephalon	Present	Regionally restricted	4G	Expression in commissural plate.

Structure	Level	Pattern	Image	Note
TS15: future hindbrain	Present	Regionally restricted	4H	Expression in dorsal neuroepithelium overlying hindbrain (neural fold).
TS15: future midbrain	Present	Regionally restricted	4H	Expression in ventral midbrain and tectum.
TS15: telencephalon	Present	Regionally restricted	4H	Expression in commissural plate.
TS15: eye	Present	Regionally restricted	4H	Expression in optic eminence.
TS15: 1st branchial groove	Present		4H	Expression in clefts between first, second and third branchial arches.
TS15: 2nd branchial groove	Present		4H	Expression in clefts between first, second and third branchial arches.

Structure	Level	Pattern	Image	Note
TS17: neural tube floor plate	Present		5E
TS17: neural tube roof plate	Present		5E
TS17: hindbrain	Present	Regionally restricted	5E	Expression in rhombic lip
TS17: branchial groove	Present		5E
TS17: eye	Present		5E
TS17: nasal pit	Present		5E

Structure	Level	Pattern	Image	Note
TS18: neural tube floor plate	Present		5F
TS18: midbrain	Present	Regionally restricted	5F	Expression in tectum.
TS18: telencephalon	Present	Regionally restricted	5F	Expression in commissural plate.
TS18: eye	Present		5F

Structure	Level	Pattern	Image	Note
TS19: neural tube floor plate	Present		5G
TS19: neural tube roof plate	Present		5G
TS19: cerebellum primordium	Present		5G	Expression in rhombic lip.
TS19: midbrain	Present	Regionally restricted	5G	Expression in tectum.
TS19: eye	Present		5G
TS19: ear	Present	Regionally restricted	5G	Expresssion in otic vesicle.
TS19: branchial groove	Present		5G
TS19: trunk	Strong	Regionally restricted	5G	Intense expression in lateral ridge tissue flanking forelimb buds, and in lateral ridge tissue between forelimb and hindlimb buds.
TS19: somite	Weak	Regionally restricted	5G	Weaker expression in a segmented pattern in somites.

Structure	Level	Pattern	Image	Note
TS19: neural tube roof plate	Present		5H
TS19: cerebellum primordium	Present		5H	Expresssion in rhombic lip.
TS19: midbrain	Present	Regionally restricted	5H	Expresssion in tectum.
TS19: ear	Present	Regionally restricted	5H	Expresssion in otic vesicle.

Structure	Level	Pattern	Image	Note
TS19: trunk	Present	Regionally restricted	6A	Expression in lateral ridge tissue.
TS19: future arm	Absent		6A	No expression in base of limb buds.
TS19: handplate	Weak	Regionally restricted	6A	Weak expression in interdigital region of distal limb bud.

Structure	Level	Pattern	Image	Note
TS21: forelimb interdigital region	Present		6D

Structure	Level	Pattern	Image	Note
TS21: forelimb interdigital region mesenchyme	Strong		6D inset

Structure	Level	Pattern	Image	Note
TS19: sclerotome	Weak	Regionally restricted	6H	Weak expression in a segmented pattern in posterior part of sclerotome.

Structure	Level	Pattern	Image	Note
TS19: sclerotome	Weak	Regionally restricted	6K	Weak expression in a segmented pattern in posterior part of sclerotome.

Structure	Level	Pattern	Image	Note
TS23: neural retina	Present	Regionally restricted	7B	Expression in neural layer of eye
TS23: retina nuclear layer	Strong	Regionally restricted	7B	Expression especially in the inner nuclear layer of retina.
TS23: lens epithelium	Weak		7B
TS23: optic nerve	Present		7B

Structure	Level	Pattern	Image	Note
TS23: vibrissa follicle	Present	Regionally restricted	7E	Expression in whisker follicle in outer root sheath along the shaft and in the layer surrounding the bulb.

Structure	Level	Pattern	Image	Note
TS23: neural retina	Present		8B
TS23: olfactory epithelium	Present		8B
TS23: tongue	Present		8B
TS23: tooth	Present		8B
TS23: telencephalon ventricular layer	Weak		8B
TS23: trigeminal ganglion	Weak		8B

Structure	Level	Pattern	Image	Note
TS25: cochlea	Present		8F
TS25: neural retina	Present		8F
TS25: olfactory epithelium	Present		8F
TS25: telencephalon ventricular layer	Weak		8F
TS25: trigeminal ganglion	Weak		8F