Prkca Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:63657 Pauken CM, et al., The expression and stage-specific localization of protein kinase C isotypes during mouse preimplantation development. Dev Biol. 2000 Jul 15;223(2):411-21
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:5492143
Gene symbol:	Prkca
Gene name:	protein kinase C, alpha

Antibody:	anti-PKCalpha
Detection system:	Secondary antibody coupled to Alexa Fluor
Assay notes:	Fixative was 0.05% glutaraldehyde, 2.0% paraformldehyde. Alexa fluor 536-conjugated secondary antibody. Confocal images.

Results

2B ©

Specimen 2B: embryonic day 1.0 (more

)
Note: 2-cell embryo.

Structure	Level	Pattern	Image	Note
TS2: 2-cell stage embryo	Present		2B

2C ©

Specimen 2C: embryonic day 1.5 (more

)
Note: 4-cell stage.

Structure	Level	Pattern	Image	Note
TS3: 4-cell stage embryo	Present		2C

2D ©

Specimen 2D: embryonic day 2.0 (more

)
Note: 8-cell embryo.

Structure	Level	Pattern	Image	Note
TS3: 8-cell stage embryo	Present		2D	Expression is enriched at the plasma membranes, excluded from nuclei.

2E ©

Specimen 2E: embryonic day 2.5 (more

)

Structure	Level	Pattern	Image	Note
TS3: compacted morula	Present		2E	Expression is enriched at the plasma membranes.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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