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Gene Expression Data
Assay Details
Assay
Reference: J:130047 Nishioka N, et al., Tead4 is required for specification of trophectoderm in pre-implantation mouse embryos. Mech Dev. 2008 Mar-Apr;125(3-4):270-83
Assay type: Immunohistochemistry
MGI Accession ID: MGI:5485174
Gene symbol: Nanog
Gene name: Nanog homeobox
Antibody: Anti-Nanog
Detection system: Secondary antibody coupled to Alexa Fluor 488
Results
Specimen 5B Nanog +/+: embryonic day 3.5 (more )
Note: 40-cell embryo. Double labeled: green - Nanog; red - Cdx2 (assay MGI:5485170).
Structure Level Pattern Image Note
TS4: inner cell mass Present 5B Nanog +/+ , 5B merge +/+ right

Specimen 5B Nanog -/-: embryonic day 3.5; Tead4tm1Hssk/Tead4tm1Hssk (more )
Note: 34-cell embryo. Double labeled: green - Nanog; red - Cdx2 (assay MGI:5485170).
Structure Level Pattern Image Note
TS4: blastocyst Strong 5B merge -/- right , 5B Nanog -/- All blastomeres showed strong expression.


Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
05/28/2024
MGI 6.13
The Jackson Laboratory