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Targeted Allele Detail
Symbol: Tead4tm1Hssk
Name: TEA domain family member 4; targeted mutation 1, Hiroshi Sasaki
MGI ID: MGI:3770620
Gene: Tead4  Location: Chr6:128204106-128277776 bp, - strand  Genetic Position: Chr6, 62.92 cM
Alliance: Tead4tm1Hssk page
Germline Transmission:  Earliest citation of germline transmission: J:130047
Parent Cell Line:  TT2 (ES Cell)
Strain of Origin:  (C57BL/6NCrlj x CBA/JNCrlj)F1
Allele Type:    Targeted (Null/knockout, Reporter)
Mutations:    Insertion, Intragenic deletion
Mutation detailsThe gene was disrupted by insertion into exon 2, which encodes the DNA-binding TEA domain of the protein, of two functional cassettes: 1) a loxP-flanked Pgk-neo-poly(A) cassette, followed by 2) a cDNA encoding the beta-geo (E. coli beta-galactosidase/neomycin resistance) fusion protein flanked 5' by an internal ribosomal entry site (IRES) and 3' by a polyadenylation signal. Reverse transcription- (RT-) PCR using primers in exons 5 and 6 detects no transcripts from the mutant allele in homozygous E3.5 embryos. Whereas immunohistochemical analysis with antisera to TEAD4 stains all cell nuclei of 34-36-cell wild-type embryos, mutant embryos produce no signal. (J:130047)
Inheritance:    Recessive
View phenotypes and curated references for all genotypes (concatenated display).
In Mice Carrying this Mutation: 36 assay results
In Structures Affected by this Mutation: 2 anatomical structures
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Tead4 Mutation:  71 strains or lines available
Original:  J:130047 Nishioka N, et al., Tead4 is required for specification of trophectoderm in pre-implantation mouse embryos. Mech Dev. 2008 Mar-Apr;125(3-4):270-83
All:  5 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
MGI 6.22
The Jackson Laboratory