Pou5f1 Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:130047 Nishioka N, et al., Tead4 is required for specification of trophectoderm in pre-implantation mouse embryos. Mech Dev. 2008 Mar-Apr;125(3-4):270-83
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:5485173
Gene symbol:	Pou5f1
Gene name:	POU domain, class 5, transcription factor 1

Antibody:	anti-Oct3/4 (C-10; sc-5279)
Detection system:	Secondary antibody coupled to Alexa Fluor 488

Results

Specimen 5B Oct3/4 +/+: embryonic day 3.5 (more

)
Note: 38-cell embryo. Double labeled: green - Pou5f1; red - Cdx2 (assay MGI:5485170).

Structure	Level	Pattern	Image	Note
TS4: inner cell mass	Present		5B Oct3/4 +/+ , 5B merge +/+ left
TS4: trophectoderm	Weak	Regionally restricted	5B merge +/+ left , 5B Oct3/4 +/+	Weak expression was detected in some cells.

Specimen 5B Oct3/4 -/-: embryonic day 3.5; Tead4^tm1Hssk/Tead4^tm1Hssk (more

)
Note: 32-cell embryo. Double labeled: green - Pou5f1; red - Cdx2 (assay MGI:5485170).

Structure	Level	Pattern	Image	Note
TS4: blastocyst	Strong		5B merge -/- left , 5B Oct3/4 -/-	All blastomeres showed strong expression.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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