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Gene Expression Data
Assay Details
Assay
Reference: J:130047 Nishioka N, et al., Tead4 is required for specification of trophectoderm in pre-implantation mouse embryos. Mech Dev. 2008 Mar-Apr;125(3-4):270-83
Assay type: Immunohistochemistry
MGI Accession ID: MGI:5485170
Gene symbol: Cdx2
Gene name: caudal type homeobox 2
Antibody: anti- Cdx2 (CDX2-88)
Detection system: Secondary antibody coupled to Alexa Fluor 594
Results
Specimen 5A wt 14-cell: embryonic day 3.0 (more )
Note: 14-cell embryo.
Structure Level Pattern Image Note
TS3: morula-stage conceptus Present 5A Cdx2 +/+ 14-cell
Specimen 5A -/- 16-cell: embryonic day 3.0; Tead4tm1Hssk/Tead4tm1Hssk (more )
Note: 16-cell embryo.
Structure Level Pattern Image Note
TS3: morula-stage conceptus Weak 5A Cdx2 -/- 16-cell
Specimen 5A wt 22-cell: embryonic day 3.5 (more )
Note: 22-cell embryo.
Structure Level Pattern Image Note
TS4: trophectoderm Present 5A Cdx2 +/+ 22-cell Expression was enhanced and gradually restricted to the outer cells that form trophectoderm.
Specimen 5A -/- 22-cell: embryonic day 3.5; Tead4tm1Hssk/Tead4tm1Hssk (more )
Note: 22-cell embryo.
Structure Level Pattern Image Note
TS4: conceptus Absent 5A Cdx2 -/- 22-cell
Specimen 5A wt 34-cell: embryonic day 3.5 (more )
Note: 34-cell embryo.
Structure Level Pattern Image Note
TS4: trophectoderm Present 5A Cdx2 +/+ 34-cell Expression was enhanced and gradually restricted to the outer cells that form trophectoderm.
Specimen 5A -/- 34-cell: embryonic day 3.5; Tead4tm1Hssk/Tead4tm1Hssk (more )
Note: 34-cell embryo.
Structure Level Pattern Image Note
TS4: conceptus Absent 5A Cdx2 -/- 34-cell
Specimen 5B left +/+: embryonic day 3.5 (more )
Note: 38-cell embryo. Double labeled: red - Cdx2; green - Pou5f1 (assay MGI:5485173).
Structure Level Pattern Image Note
TS4: trophectoderm Present 5B merge +/+ left , 5B Cdx2 +/+ left
Specimen 5B left -/-: embryonic day 3.5; Tead4tm1Hssk/Tead4tm1Hssk (more )
Note: 32-cell embryo. Double labeled: red - Cdx2; green - Pou5f1 (assay MGI:5485173).
Structure Level Pattern Image Note
TS4: trophectoderm Absent 5B Cdx2 -/- left , 5B merge -/- left
Specimen 5B right +/+: embryonic day 3.5 (more )
Note: 40-cell embryo. Double labeled: red - Cdx2; green - Nanog (assay MGI:5485174).
Structure Level Pattern Image Note
TS4: trophectoderm Present 5B merge +/+ right , 5B Cdx2 +/+ right
Specimen 5B right -/-: embryonic day 3.5; Tead4tm1Hssk/Tead4tm1Hssk (more )
Note: 34-cell embryo. Double labeled: red - Cdx2; green - Nanog (assay MGI:5485174).
Structure Level Pattern Image Note
TS4: trophectoderm Absent 5B Cdx2 -/- right , 5B merge -/- right
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/28/2024
MGI 6.13 		The Jackson Laboratory