Probe:	Alx3 cDNA1
Probe preparation:	Antisense RNA
Visualized with:	Autoradiography

Structure	Level	Pattern	Image	Note
TS20: head mesenchyme	Present	Regionally restricted	5A	Expression is present in a thin layer of mesenchymal cells surrounding the telencephalic vesicles; expression in the remaining mesoderm of the cranial vault is generally low but above background.

Structure	Level	Pattern	Image	Note
TS17: head	Present	Regionally restricted	5B	Expression is present in craniofacial structures; it is particularly high in the lower frontal part of the head, with a rostral border near the level of the eye.
TS17: head mesenchyme	Weak	Regionally restricted	5B	Expression is present mesoderm of the cranial vault.
TS17: 1st branchial arch	Present	Regionally restricted	5B	Expression is located distally.
TS17: 2nd branchial arch	Present	Regionally restricted	5B	Expression is located distally.

Structure	Level	Pattern	Image	Note
TS20: fronto-nasal process mesenchyme	Present	Regionally restricted	5C	Expression is present in the neural-crest derived mesenchyme in the nasal process.
TS20: jaw primordium mesenchyme	Present	Regionally restricted	5C	Expression is present in the neural-crest derived mesenchyme of the developing jaws.
TS20: tongue mesenchyme	Present		5C	Expression is present in the neural-crest derived mesenchyme of the distal part of the tongue.
TS20: mandibular process	Strong	Regionally restricted	5C	Expression is high in the area surrounding Meckel's cartilage and elevated near the developing incisor and molar buds. It is not present in the ossifying regions.
TS20: Meckel's cartilage	Absent		5C

Structure	Level	Pattern	Image	Note
TS22: head	Present	Regionally restricted	5D	Expression is present in craniofacial structures; it is particularly high in the lower frontal part of the head, with a rostral border near the level of the eye.
TS22: head mesenchyme	Present	Regionally restricted	5D	Expression is present in a thin layer of mesenchymal cells surrounding the telencephalic vesicles; expression in the remaining mesoderm of the cranial vault is generally low but above background.
TS22: nasal mesenchyme	Strong	Regionally restricted	5D	Expression is present in the mesenchyme around the nasal cavity; there is a lack of expression at more lateral positions.
TS22: nasal septum mesenchyme	Strong		5D

Structure	Level	Pattern	Image	Note
TS22: nasal mesenchyme	Present		5E	Expression is present in the neural-crest derived mesenchyme in the nasal process.
TS22: lower jaw mesenchyme	Present		5E	Expression is present in the neural-crest derived mesenchyme of the developing jaws.
TS22: upper jaw mesenchyme	Present		5E	Expression is present in the neural-crest derived mesenchyme of the developing jaws.
TS22: premaxilla	Present		5E	Expression is present in the premaxilla but absent from the secondary plate.
TS22: lower jaw	Strong	Regionally restricted	5E	Expression is high in the area surrounding Meckel's cartilage and elevated near the developing incisor and molar buds. It is not present in ossifying areas.
TS22: Meckel's cartilage	Absent		5E

Structure	Level	Pattern	Image	Note
TS22: nasal mesenchyme	Strong	Regionally restricted	5F	Expression is present in the mesenchyme around the nasal cavity; there is a lack of expression at more lateral positions.
TS22: nasal septum mesenchyme	Strong		5F
TS22: lower jaw mesenchyme	Present		5F	Expression is present in the neural-crest derived mesenchyme of the developing jaws.
TS22: upper jaw mesenchyme	Present		5F	Expression is present in the neural-crest derived mesenchyme of the developing jaws.
TS22: tongue mesenchyme	Present		5F	Expression is present in the neural-crest derived mesenchyme of the distal part of the tongue.
TS22: lower jaw	Strong	Regionally restricted	5F	Expression is high in the area surrounding Meckel's cartilage and elevated near the developing incisor and molar buds. It is not present in the ossifying areas.
TS22: Meckel's cartilage	Absent		5F

Structure	Level	Pattern	Image	Note
TS20: chondrocranium	Present	Regionally restricted	5G	Expression is present in the neural-crest derived basisphenoid and absent in the mesoderm-derived basioccipital.

Structure	Level	Pattern	Image	Note
TS22: limb mesenchyme	Present	Regionally restricted	6A	Expression is present underneath the surface of the limbs, following the profile of the surface epithelium.
TS22: limb cartilage condensation	Absent		6A

Structure	Level	Pattern	Image	Note
TS17: tail	Present	Regionally restricted	6B	Expression in the tail is ventrally concentrated; this expression appears to extend to the ectoderm.
TS17: tail mesenchyme	Present		6B
TS17: body wall mesenchyme	Present		6B

Structure	Level	Pattern	Image	Note
TS20: tail	Present	Regionally restricted	6C	Expression in the tail is ventrally concentrated; this expression appears to extend to the ectoderm.
TS20: tail mesenchyme	Present		6C
TS20: body wall mesenchyme	Present		6C	The body wall is still open at this site, which is reflected by an interruption of the expression domain.

Structure	Level	Pattern	Image	Note
TS20: genital tubercle	Present	Regionally restricted	6D	Expression is present in the genital eminence surrounds the urogenital sinuses.
TS20: tail	Present	Regionally restricted	6D	Expression in the tail is ventrally concentrated; this expression appears to extend to the ectoderm.
TS20: tail mesenchyme	Present		6D

Structure	Level	Pattern	Image	Note
TS22: genital tubercle	Present	Regionally restricted	6E	Expression is present in the genital eminence surrounds the urogenital sinuses.
TS22: tail mesenchyme	Present	Regionally restricted	6E	Expression in the tail is ventrally concentrated.

Structure	Level	Pattern	Image	Note
TS21: genital tubercle	Present	Regionally restricted	6F	Expression is present in the genital eminence surrounds the urogenital sinuses.
TS21: tail mesenchyme	Present	Regionally restricted	6F	Expression in the tail is ventrally concentrated.

Structure	Level	Pattern	Image	Note
TS19: intraembryonic coelom	Present	Regionally restricted	6G	Expression is present in the progenitor of the pleuroperitoneal membrane.
TS19: trunk mesenchyme	Weak	Regionally restricted	6G	There is expression surrounding the mesonephric duct.

Structure	Level	Pattern	Image	Note
TS17: pericardial component	Present			Expression is present in the pericardial sac membrane.
TS17: intraembryonic coelom	Present			Expression is present in the pleuro-peritoneal septum.
TS17: peritoneal component	Present			Expression is present in the peritoneal membrane in which the liver is contained.

Structure	Level	Pattern	Image	Note
TS19: pericardial component	Present			Expression is present in the pericardial sac membrane.
TS19: peritoneal component	Present			Expression is present in the peritoneal membrane in which the liver is contained.

Structure	Level	Pattern	Image	Note
TS22: pancreas	Absent		7B , 7A

Structure	Level	Pattern	Image	Note
TS17: trunk mesenchyme	Trace	Regionally restricted	8E	Expression is barely detectable in the intermediate mesoderm.
TS17: limb	Present		8E
TS17: tail	Present		8E

Structure	Level	Pattern	Image	Note
TS17: embryo	Present		8G

Structure	Level	Pattern	Image	Note
TS21: rib cartilage condensation	Absent		8I