Probe:	MGC clone 8241
Probe preparation:	Antisense labelled with S35 RNA

Structure	Level	Pattern	Image	Note
TS13: migrating neural crest	Absent

Structure	Level	Pattern	Image	Note
TS15: migrating neural crest	Absent

Structure	Level	Pattern	Image	Note
TS17: migrating neural crest	Absent

Structure	Level	Pattern	Image	Note
TS19: telencephalon	Strong	Regionally restricted	2A	Expression was detected in the ventral telencephalon.

Structure	Level	Pattern	Image	Note
TS19: inner ear epithelium	Present	Regionally restricted	2F	Expression was confined to a subset of epithelial cells that may represent the neurosensory patches.

Structure	Level	Pattern	Image	Note
TS19: cranial ganglion	Present		2G
TS19: head mesenchyme	Present		2G
TS19: blood vessel	Present	Regionally restricted	2G	Expression was detected in blood vessels associated with the leptomeninges.

Structure	Level	Pattern	Image	Note
TS19: diencephalon	Strong
TS19: midbrain	Strong
TS19: future pituitary	Moderate
TS19: olfactory epithelium	Strong	Regionally restricted		High expression was detected in the olfactory epithelium and olfactory ensheathing cells.
TS19: tongue	Moderate	Regionally restricted		Expression was detected in the tongue epithelium.
TS19: oral epithelium	Present	Regionally restricted		Expression was detected in the epithelium of the floor of the oral cavity.

Structure	Level	Pattern	Image	Note
TS20: diencephalon	Strong		2K
TS20: pituitary gland	Strong		2K
TS20: telencephalon	Strong		2K
TS20: trigeminal ganglion	Present	Single cells	2K

Structure	Level	Pattern	Image	Note
TS20: diencephalon	Strong		2P
TS20: pituitary gland	Strong		2P
TS20: telencephalon	Strong		2P
TS20: eye	Strong	Regionally restricted	2P	Expression was strong in cells underlying the optic stalk.
TS20: retina	Strong		2P

Structure	Level	Pattern	Image	Note
TS20: spinal cord roof plate	Present		2P'

Structure	Level	Pattern	Image	Note
TS20: hyaloid vascular plexus	Strong		2Q
TS20: retina	Strong		2Q

Structure	Level	Pattern	Image	Note
TS20: blood vessel	Present		2R
TS20: cranial ganglion	Present		2R
TS20: inner ear	Present		2R
TS20: olfactory epithelium	Present		2R	Expression was detected in the olfactory epithelium and the olfactory ensheathing cells.

Structure	Level	Pattern	Image	Note
TS20: telencephalon	Present	Regionally restricted	3A/A'	Expression was detected in the neuroepithelium of the septum and pallidum, as well as the olfactory bulb.
TS20: cerebellum primordium	Present		3A/A'
TS20: corpus striatum	Present		3A/A'
TS20: hypothalamus	Present	Regionally restricted	3A/A'	Expression was detected in the neuroepithelium of the preoptic area and the hypothalamus.
TS20: midbrain	Present	Regionally restricted	3A/A'	Expression was detected in the neuroepithelium of the posterior tegmentum and the inferior tectum (inferior colliculus).
TS20: midbrain-hindbrain junction	Present	Regionally restricted	3A/A'	Expression was detected in the ventral and dorsal isthmus.

Structure	Level	Pattern	Image	Note
TS20: telencephalon	Present	Regionally restricted	3F/F'	Expression was detected in the neuroepithelium of the septum and pallidum, as well as the olfactory bulb.
TS20: corpus striatum	Present		3F/F'
TS20: hypothalamus	Present	Regionally restricted	3F/F'	Expression was detected in the neuroepithelium of the preoptic area and the hypothalamus.
TS20: midbrain	Present	Regionally restricted	3F/F'	Expression was detected in the neuroepithelium of the posterior tegmentum and the inferior tectum (inferior colliculus).
TS20: midbrain-hindbrain junction	Present	Regionally restricted	3F/F'	Expression was detected in the ventral and dorsal isthmus. The expression level in the ventral domain was reduced compared to E12.0.
TS20: cerebellum primordium	Present		3F/F'	The expression level was reduced compared to E12.0.

Structure	Level	Pattern	Image	Note
TS21: telencephalon	Present	Regionally restricted	4A	Expression was detected in the olfactory bulb, the septum and the pallidum.
TS21: corpus striatum	Present		4A
TS21: hypothalamus	Present		4A
TS21: thalamus	Present		4A
TS21: brain meninges	Present		4A
TS21: blood vessel	Present	Regionally restricted	4A	Expression was detected in the blood vessels associated with meninges.

Structure	Level	Pattern	Image	Note
TS21: hyaloid vascular plexus	Strong		4F
TS21: retina	Strong		4F
TS21: eye	Present	Regionally restricted	4F	Expression was detected in the mesenchyme adjacent to the cornea.
TS21: brain meninges	Present		4F
TS21: hypothalamus	Present		4F
TS21: lens epithelium	Present		4F
TS21: thalamus	Present		4F

Structure	Level	Pattern	Image	Note
TS22: hypothalamus	Ambiguous		4K	Expression in the hypothalamus dwindled at E14.5.
TS22: brain meninges	Present		4K

Structure	Level	Pattern	Image	Note
TS22: hyaloid vascular plexus	Strong		4P
TS22: retina	Strong	Regionally restricted	4P	The outer layer of the retina displayed the highest expression levels.
TS22: eye	Present	Regionally restricted	4P	Expression was detected in the mesenchyme adjacent to the cornea.
TS22: lens epithelium	Present		4P

Structure	Level	Pattern	Image	Note
TS26: olfactory cortex ventricular layer	Strong		4S
TS26: main olfactory bulb	Present	Scattered	4S	Scattered cells in the differentiating fields were positive.
TS26: conjunctival sac	Present	Regionally restricted	4S	Expression was detected in the conjunctival sac lining.
TS26: lens epithelium	Present		4S
TS26: retina	Present		4S

Structure	Level	Pattern	Image	Note
TS26: blood vessel	Present		4U
TS26: organ of Corti	Present		4U
TS26: cochlear duct	Present	Regionally restricted	4U	Expression was detected in the cochlear stria vascularis.

Structure	Level	Pattern	Image	Note
TS26: midbrain tegmentum	Present
TS26: telencephalon	Present

Structure	Level	Pattern	Image	Note
TS19: oral epithelium	Weak	Regionally restricted	5B , 5A	Low levels of expression were detected in the dental epithelium.
TS19: jaw primordium mesenchyme	Present	Regionally restricted	5B , 5A	Expression was detected in a broad mesenchymal domain adjacent to the dental epithelium.

Structure	Level	Pattern	Image	Note
TS20: vibrissa follicle	Absent
TS20: respiratory system epithelium	Present

Structure	Level	Pattern	Image	Note
TS22: cervical loop	Present		5F	Expression was detected in the elongating cervical loops.
TS22: molar mesenchyme	Present		5F
TS22: enamel knot	Absent		5F

Structure	Level	Pattern	Image	Note
TS23: skin	Present	Regionally restricted		Expression was detected in the suprabasal layers of the skin.

Structure	Level	Pattern	Image	Note
TS26: epidermis	Present	Regionally restricted	5I	Expression was detected in the suprabasal layers of the epidermis.
TS26: cervical loop	Present	Non-Uniform	5I	Expression was strong in the buccal cervical loop, whereas the lingual cervical loop was only slightly above background in the incisor.
TS26: facial bone	Present	Regionally restricted	5I	Expression was detected in regions of membrane and endochondral bone formation.

Structure	Level	Pattern	Image	Note
TS26: molar mesenchyme	Present	Regionally restricted	5M	Expression was high in the mesenchyme of nascent cusps but was more scarce in the fully developed cusps.
TS26: facial bone	Present	Regionally restricted	5M	Expression was detected in regions of membrane and endochondral bone formation.

Structure	Level	Pattern	Image	Note
TS26: molar enamel organ	Present	Non-Uniform	5Q	Expression was weak in the inner enamel epithelium of incisors.
TS26: molar mesenchyme	Present	Regionally restricted	5Q	Expression was high in the mesenchyme of nascent cusps but was more scarce in the fully developed cusps.
TS26: dental papilla	Trace		5Q
TS26: tooth	Present	Regionally restricted	5Q	Expression was detected in differentiating odontoblasts.

Structure	Level	Pattern	Image	Note
TS28: incisor	Present	Regionally restricted	5R	Expression was detected in odontoblasts, some blood vessels and scattered pulpal cells adjacent to blood vessels.
TS28: incisor dental pulp	Present	Scattered	5R
TS28: viscerocranium	Present	Regionally restricted	5R	Expression was detected in regions of membrane and endochondral bone formation.

Structure	Level	Pattern	Image	Note
TS28: molar	Present	Regionally restricted	5V	Expression was detected in odontoblasts, some blood vessels and scattered pulpal cells adjacent to blood vessels. Secretory ameloblasts were devoid of expression.
TS28: molar dental pulp	Present	Scattered	5V
TS28: viscerocranium	Present	Regionally restricted	5V	Expression was detected in regions of membrane and endochondral bone formation.

Structure	Level	Pattern	Image	Note
TS19: palatal shelf	Present		6A

Structure	Level	Pattern	Image	Note
TS19: olfactory epithelium	Strong		6A'
TS19: nose	Strong	Regionally restricted	6A'	Some cell aggregates within the mesenchyme underlying the olfactory pits were strongly stained.

Structure	Level	Pattern	Image	Note
TS20: olfactory epithelium	Present	Non-Uniform	6B	Expression was detected in the olfactory epithelium and olfactory ensheathing cells. Expression was strong in some areas of the epithelium, while other areas were weaker.
TS20: head mesenchyme	Present	Regionally restricted	6D , 6B , 6C	Expression was detected in cells along the trigeminal nerve axons and in mesenchymal condensates adjacent to blood vessels.
TS20: blood vessel	Present		6D , 6B

Structure	Level	Pattern	Image	Note
TS20: oral epithelium	Present	Regionally restricted	6F	Expression was detected in the oral epithelium but was excluded from the tooth anlagen. Expression was in the floor of the oral cavity.
TS20: olfactory epithelium	Present	Non-Uniform	6F	Expression was detected in the olfactory epithelium and olfactory ensheathing cells. Expression was strong in some areas of the epithelium, while other areas were weaker.
TS20: primary palate	Present		6F
TS20: palatal shelf epithelium	Present	Regionally restricted	6F	Expression was detected in the epithelia of nascent palatal shelves.
TS20: nasal septum epithelium	Absent		6F

Structure	Level	Pattern	Image	Note
TS21: vibrissa follicle	Absent
TS21: vomeronasal organ	Present
TS21: blood vessel	Present	Regionally restricted		Expression was detected in the walls of lingual blood vessels flanking the rostral-most portion of the genioglossus muscles.
TS21: submandibular gland primordium epithelium	Present			Expression was higher in the submandibular gland epithelium than in the mesenchyme.
TS21: submandibular gland primordium mesenchyme	Present			Expression was higher in the submandibular gland epithelium than in the mesenchyme.

Structure	Level	Pattern	Image	Note
TS21: tooth	Absent		6K
TS21: olfactory epithelium	Present		6K	Expression was similar to E12.5.
TS21: primary palate	Present		6K	Expression was similar to E12.5.
TS21: palatal shelf epithelium	Weak		6K	Expression in the anterior and mid-palate palatal shelf epithelium decreased.
TS21: palatal rugae	Present	Regionally restricted	6K	Expression was detected in the epithelium of the rugae palatinae.
TS21: head	Present	Regionally restricted	6K	Expression was detected in olfactory ensheathing cells in the lamina propria underlying the olfactory epithelium and in cells associated with axons of the olfactory nerve.
TS21: tongue mesenchyme	Present	Regionally restricted	6K	Expression was detected in mesenchymal cells of the lingual septum.
TS21: facial bone primordium	Present	Regionally restricted	6K	Expression was detected in osteoblasts within areas of endochondral and intramembranous ossification. The perichondrium was moderately labeled.

Structure	Level	Pattern	Image	Note
TS21: palatal shelf epithelium	Strong	Regionally restricted	6P	Strong expression was detected in the medial edge epithelium of the posterior palatal shelves.
TS21: tongue mesenchyme	Present	Regionally restricted	6P	Expression was detected in mesenchyme underlying the epithelium of the posterior tongue, some mesenchymal condensates adjacent to the epithelium of the lateral surface of the tongue, and mesenchyme underlying the vallate papilla epithelium.
TS21: facial bone primordium	Present	Regionally restricted	6P	Expression was detected in osteoblasts within areas of endochondral and intramembranous ossification. The perichondrium was moderately labeled.

Structure	Level	Pattern	Image	Note
TS22: head mesenchyme	Present	Regionally restricted	6E , 6Q	Expression was detected in mesenchymal condensates adjacent to blood vessels. The condensates were associated with a bone matrix.
TS22: palatal shelf epithelium	Present	Regionally restricted	6Q	Expression was increased in the medial edge epithelium just before contact between the palatal shelves.
TS22: nasal septum epithelium	Present		6Q
TS22: olfactory epithelium	Strong	Regionally restricted	6Q	High expression was detected in the respiratory epithelium in the nasal cavity and in the olfactory ensheathing cells.
TS22: blood vessel	Strong	Regionally restricted	6Q	Expression was detected in lingual blood vessels in the tongue.
TS22: tongue mesenchyme	Strong	Regionally restricted	6Q	Clusters of mesenchymal cells at the posterior and lateral portion of the tongue exhibited strong expression.
TS22: facial bone primordium	Strong	Regionally restricted	6Q	Expression was detected in osteoblasts, hypertrophic chondrocytes and the perichondrium.
TS22: submandibular gland primordium epithelium	Present		6Q	Expression was higher in the submandibular gland epithelium than in the mesenchyme.
TS22: submandibular gland primordium mesenchyme	Present		6Q	Expression was higher in the submandibular gland epithelium than in the mesenchyme.

Structure	Level	Pattern	Image	Note
TS22: palatal shelf epithelium	Present	Regionally restricted	6T	Expression was high in the medial epithelial seam following contact of the palatal shelves.
TS22: olfactory epithelium	Strong	Regionally restricted	6T	High expression was detected in the respiratory epithelium in the nasal cavity and in the olfactory ensheathing cells.
TS22: blood vessel	Strong	Regionally restricted	6T	Expression was detected in lingual blood vessels in the tongue.
TS22: tongue mesenchyme	Strong	Regionally restricted	6T	Clusters of mesenchymal cells at the posterior and lateral portion of the tongue exhibited strong expression.
TS22: facial bone primordium	Strong	Regionally restricted	6T	Expression was detected in osteoblasts, hypertrophic chondrocytes and the perichondrium.
TS22: submandibular gland primordium epithelium	Present		6T	Expression was higher in the submandibular gland epithelium than in the mesenchyme.
TS22: submandibular gland primordium mesenchyme	Present		6T	Expression was higher in the submandibular gland epithelium than in the mesenchyme.

Structure	Level	Pattern	Image	Note
TS23: facial bone primordium	Strong	Regionally restricted	6Z	Expression was detected in osteoblasts, hypertrophic chondrocytes and the perichondrium.

Structure	Level	Pattern	Image	Note
TS23: submandibular gland primordium epithelium	Present			Expression was higher in the submandibular gland epithelium than in the mesenchyme.
TS23: submandibular gland primordium mesenchyme	Present			Expression was higher in the submandibular gland epithelium than in the mesenchyme.

Structure	Level	Pattern	Image	Note
TS26: nasal cavity respiratory epithelium	Strong
TS26: olfactory epithelium	Weak
TS26: blood vessel	Present	Regionally restricted		Expression was detected in lingual blood vessels.