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Gene Expression Data
Assay Details
Assay
Reference: J:51994 Retaux S, et al., Lhx9: a novel LIM-homeodomain gene expressed in the developing forebrain. J Neurosci. 1999 Jan 15;19(2):783-93
Assay type: RNA in situ
MGI Accession ID: MGI:5469494
Gene symbol: En2
Gene name: engrailed 2
Probe: Bluescript-En2
Probe preparation: Antisense labelled with radioactivity RNA
Visualized with: Autoradiography
Assay notes: Fixative was in 0.1 M 4-morpholinepropanesulfonic acid, pH 7.4, 2 mM EGTA, 1 mM MgSO4 , and 3.7% formaldehyde.
Results
Specimen 2F: embryonic day 12.5 (more )
Structure Level Pattern Image Note
TS20: hindbrain Present 2F
Specimen 2G: embryonic day 12.5 (more )
Structure Level Pattern Image Note
TS20: diencephalon Present 2G
TS20: midbrain Present 2G
Specimen 4K: embryonic day 13.5 (more )
Structure Level Pattern Image Note
TS21: metencephalon Present 4K
TS21: midbrain Present 4K
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/18/2025
MGI 6.24 		The Jackson Laboratory