Probe:	En2 cDNA1
Probe preparation:	Antisense labelled with S35 RNA
Visualized with:	Autoradiography

Structure	Level	Pattern	Image	Note
TS11: embryo	Absent

Structure	Level	Pattern	Image	Note
TS12: future brain	Present	Regionally restricted	1a left	Expression is present in a band in the neural folds at the level of the foregut pocket.

Structure	Level	Pattern	Image	Note
TS12: future brain	Present	Regionally restricted	1b left	Expression is present in a band in the neural folds at the level of the foregut pocket.

Structure	Level	Pattern	Image	Note
TS20: hindbrain	Strong	Regionally restricted	2a left	Expression is present in a ring in the region that will later form the pons, cerebellum, part of the colliculi, and the periaqueductal gray.
TS20: midbrain	Strong	Regionally restricted	2a left	Expression is present in a ring in the region that will later form the pons, cerebellum, part of the colliculi, and the periaqueductal gray.
TS20: midbrain-hindbrain junction	Strong	Regionally restricted	2a left	Expression is present in a ring in the region that will later form the pons, cerebellum, part of the colliculi, and the periaqueductal gray.
TS20: cerebellum primordium	Strong		2a left
TS20: pons	Strong		2a left

Structure	Level	Pattern	Image	Note
TS20: hindbrain	Strong	Regionally restricted	2b left	Expression is present in a ring in the region that will later form the pons, cerebellum, part of the colliculi, and the periaqueductal gray.
TS20: midbrain	Strong	Regionally restricted	2b left	Expression is present in a ring in the region that will later form the pons, cerebellum, part of the colliculi, and the periaqueductal gray.
TS20: midbrain-hindbrain junction	Strong	Regionally restricted	2b left	Expression is present in a ring in the region that will later form the pons, cerebellum, part of the colliculi, and the periaqueductal gray.
TS20: cerebellum primordium	Strong		2b left
TS20: pons	Strong		2b left
TS20: spinal cord	Absent		2b left
TS20: tail	Absent		2b left
TS20: vertebral cartilage condensation	Absent		2b left

Structure	Level	Pattern	Image	Note
TS20: hindbrain	Strong	Regionally restricted	3c left	Expression is present in a ring in the region that will later form the pons, cerebellum, part of the colliculi, and the periaqueductal gray.
TS20: midbrain	Strong	Regionally restricted	3c left	Expression is present in a ring in the region that will later form the pons, cerebellum, part of the colliculi, and the periaqueductal gray.
TS20: midbrain-hindbrain junction	Strong	Regionally restricted	3c left	Expression is present in a ring in the region that will later form the pons, cerebellum, part of the colliculi, and the periaqueductal gray.
TS20: cerebellum primordium	Strong		3c left
TS20: pons	Strong		3c left
TS20: dermomyotome	Absent		3c left
TS20: limb	Absent		3c left
TS20: spinal cord	Absent		3c left
TS20: tail	Absent		3c left

Structure	Level	Pattern	Image	Note
TS20: hindbrain	Strong	Regionally restricted	3d left	Expression is present in a ring in the region that will later form the pons, cerebellum, part of the colliculi, and the periaqueductal gray.
TS20: midbrain	Strong	Regionally restricted	3d left	Expression is present in a ring in the region that will later form the pons, cerebellum, part of the colliculi, and the periaqueductal gray.
TS20: midbrain-hindbrain junction	Strong	Regionally restricted	3d left	Expression is present in a ring in the region that will later form the pons, cerebellum, part of the colliculi, and the periaqueductal gray.
TS20: cerebellum primordium	Strong		3d left
TS20: pons	Strong		3d left
TS20: adenohypophysis	Present		3d left	Expression is present in cells around Rathke's pouch.
TS20: dermomyotome	Absent		3d left
TS20: limb	Absent		3d left
TS20: spinal cord	Absent		3d left
TS20: tail	Absent		3d left

Structure	Level	Pattern	Image	Note
TS23: hindbrain	Strong		4c left
TS23: midbrain	Strong		4c left	Regions of expression include the periaqueductal gray.
TS23: cerebellum	Present	Widespread	4c left
TS23: inferior colliculus	Present	Widespread	4c left
TS23: pons	Present		4c left

Structure	Level	Pattern	Image	Note
TS23: hindbrain	Strong		4d left
TS23: midbrain	Strong		4d left
TS23: cerebellum	Present	Widespread	4d left
TS23: inferior colliculus	Present	Widespread	4d left

Structure	Level	Pattern	Image	Note
TS25: cerebellum	Present	Widespread	5a left	Areas of expression include the external granule cell layer.
TS25: pons	Present	Regionally restricted	5a left	Expression extends from the junction of the cerebellum and pons rostrally into the midbrain. It is also present in a group of cells just under the caudal edge of the cerebellum.
TS25: midbrain	Present		5a left

Structure	Level	Pattern	Image	Note
TS25: cerebellum	Present	Widespread	5b left	Areas of expression include the external granule cell layer.
TS25: pons	Present	Regionally restricted	5b left	Expression extends from the junction of the cerebellum and pons rostrally into the midbrain. It is also present in a group of cells just under the caudal edge of the cerebellum.

Structure	Level	Pattern	Image	Note
TS28: cerebellum granule cell layer	Strong		6b left
TS28: periaqueductal grey	Present	Regionally restricted	6b left	Expression is present in the ventral, but not dorsal, periaqueductal gray; it extends forward from under the rostral portion of the cerebellum to the midbrain.
TS28: pons	Present		6b left	Areas of expression include the raphe linearis.
TS28: interpeduncular nucleus	Present		6b left

Structure	Level	Pattern	Image	Note
TS28: tectum	Absent		6c left	There is no expression in the colliculi.
TS28: substantia nigra	Present		6c left
TS28: pons	Present		6c left