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Gene Expression Data
Assay Details
Assay
Reference: J:9911 Davidson D, et al., A gene with sequence similarity to Drosophila engrailed is expressed during the development of the neural tube and vertebrae in the mouse. Development. 1988 Oct;104(2):305-16
Assay type: RNA in situ
MGI Accession ID: MGI:5469206
Gene symbol: En2
Gene name: engrailed 2
Probe: p-lambda-4L7
Probe preparation: Antisense labelled with S35 RNA
Visualized with: Autoradiography
Assay notes: The probe used for this assay detects the expression of both family members.
Results
Specimen Table 1: embryonic day 12.5 (more )
Structure Level Pattern Image Note
TS20: midbrain Present
TS20: midbrain-hindbrain junction Present
TS20: hindbrain floor plate Absent Based on experiments using gene-specific probes, the observed expresion in the floor of the hindbrain is believed to be due to En1.
TS20: spinal cord Absent Based on experiments using gene-specific probes, the observed expresion in the neural tube is believed to be due to En1.
TS20: dermomyotome Absent Based on experiments using gene-specific probes, the observed expresion in the dermotome is believed to be due to En1.
TS20: sclerotome Absent Based on experiments using gene-specific probes, the observed expresion in the sclerotome of developing centra is believed to be due to En1.
TS20: vertebral cartilage condensation Absent Based on experiments using gene-specific probes, the observed expresion in the costal processes and neural arches is believed to be due to En1.
TS20: head mesenchyme Absent Based on experiments using gene-specific probes, the observed expresion in the facial mesenchyme is believed to be due to En1.
TS20: limb epithelium Absent Based on experiments using gene-specific probes, the observed expresion in the limb bud ectoderm is believed to be due to En1.
TS20: body wall Ambiguous Based on experiments using gene-specific probes, the observed expresion in the thoracic body wall may be an artefact.

Specimen 2A/B: embryonic day 12.5 (more )
Structure Level Pattern Image Note
TS20: spinal cord Absent 2A/B Expression is present in the ventral part of the neural tube from the junction with the hindbrain to at least the level of the 30th segment posterior to the atlas. Based on experiments using gene-specific probes, this is believed to be due to En1.
TS20: head mesenchyme Absent 2A/B Expression is present in the facial mesenchyme anterior to the forebrain, above the developing snout; there is no expression in the lateral parts of face. Based on experiments using gene-specific probes, this is believed to be due to En1.
TS20: vibrissa Absent 2A/B
TS20: dermomyotome Absent 2A/B There is a complex pattern of expression. Based on experiments using gene-specific probes, this is believed to be due to En1.
TS20: sclerotome Absent 2A/B There is a complex pattern of expression. Based on experiments using gene-specific probes, this is believed to be due to En1.
TS20: midbrain Present 2A/B
TS20: midbrain-hindbrain junction Present 2A/B
TS20: vertebral cartilage condensation Absent 2A/B Based on experiments using gene-specific probes, the observed expression is believed to be due to En1.

Specimen 3A/B: embryonic day 12.5 (more )
Note: sagittal section
Structure Level Pattern Image Note
TS20: midbrain Present Regionally restricted 3A/B Expression is present in discrete domains in the germinal and intermediate zones.
TS20: midbrain-hindbrain junction Present Regionally restricted 3A/B Expression is present in the roof.
TS20: hindbrain floor plate Absent 3A/B Expression is observed in the germinal and intermediate zones in the anterior floor of the hindbrain. Based on experiments using gene-specific probes, this is believed to be due to En1.
TS20: midbrain floor plate Strong 3A/B
TS20: pons Strong 3A/B

Specimen 4A/B: embryonic day 12.5 (more )
Note: sagittal section in the posterior region of the embryo
Structure Level Pattern Image Note
TS20: spinal cord Absent 4A/B Expression is present in the ventral part of the neural tube from the junction with the hindbrain to at least the level of the 30th segment posterior to the atlas. Based on experiments using gene-specific probes, this is believed to be is due to En1.

Specimen 5A/B: embryonic day 12.5 (more )
Note: transverse section from the posterior region of the hindbrain
Structure Level Pattern Image Note
TS20: hindbrain Absent 5A/B Expression is present in discrete domains in the germinal and intermediate zones. Based on experiments using gene-specific probes, this is believed to be due to En1.

Specimen 5C/D: embryonic day 12.5 (more )
Note: transverse section at the thoracic level
Structure Level Pattern Image Note
TS20: spinal cord Absent 5C/D Expression is present in bilaterally symmetrical regions in the ventral spinal cord. It is most intense in a restricted, midlateral region of the germinal zone. Based on experiments using gene-specific probes, this is believed to be due to En1.

Specimen 6A/B: embryonic day 12.5 (more )
Note: transverse section
Structure Level Pattern Image Note
TS20: tail dermomyotome Absent 6A/B Based on experiments using gene-specific probes, the observed expression is believed to be due to En1.
TS20: tail spinal cord Absent 6A/B There is no expression in the caudal part of the tail.

Specimen 7A/B: embryonic day 12.5 (more )
Note: frontal section at the notochordal level
Structure Level Pattern Image Note
TS20: sclerotome Absent 7A/B Expression is present in the developing centrum. Based on experiments using gene-specific probes, this is believed to be due to En1. There is no expression in the prospective intervertebral disc area.
TS20: cervical vertebral cartilage condensation Absent 7A/B Expression is present in the developing costal processes. Based on experiments using gene-specific probes, this is believed to be due to En1.
TS20: thoracic vertebral cartilage condensation Absent 7A/B Expression is present in the developing costal processes. Based on experiments using gene-specific probes, this is believed to be due to En1.

Specimen 7C/D: embryonic day 12.5 (more )
Note: frontal section at the notochordal level
Structure Level Pattern Image Note
TS20: sclerotome Absent 7C/D Expression is present in the developing centrum. Based on experiments using gene-specific probes, this is believed to be due to En1. There is no expression in the prospective intervertebral disc area.
TS20: cervical vertebral cartilage condensation Absent 7C/D Expression is present in the developing costal processes. Based on experiments using gene-specific probes, this is believed to be due to En1.
TS20: thoracic vertebral cartilage condensation Absent 7C/D Expression is present in the developing costal processes. Based on experiments using gene-specific probes, this is believed to be due to En1.

Specimen 8A/B: embryonic day 12.5 (more )
Note: sagittal section in the lower thoracic and lumbar region
Structure Level Pattern Image Note
TS20: lumbar vertebral cartilage condensation Absent 8A/B Expression is present in segmental blocks of mesenchyme in the positions expected of the developing costal process. Based on experiments using gene-specific probes, this is believed to be due to En1.
TS20: thoracic vertebral cartilage condensation Absent 8A/B Expression is present in segmental blocks of mesenchyme in the positions expected of the developing costal process. Based on experiments using gene-specific probes, this is believed to be due to En1.

Specimen 8C/D: embryonic day 12.5 (more )
Note: sagittal section in the cervical and upper thoracic region
Structure Level Pattern Image Note
TS20: cervical vertebral cartilage condensation Absent 8C/D Expression is present in the neural arches; it is most intense in the anterior part. Based on experiments using gene-specific probes, this expression is believed to be due to En1.
TS20: thoracic vertebral cartilage condensation Absent 8C/D Expression is present in the neural arches; it is most intense in the anterior part. Based on experiments using gene-specific probes, this expression is believed to be due to En1.


Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
03/25/2025
MGI 6.24
The Jackson Laboratory