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Gene Expression Data
Assay Details
Assay
Reference: J:9911 Davidson D, et al., A gene with sequence similarity to Drosophila engrailed is expressed during the development of the neural tube and vertebrae in the mouse. Development. 1988 Oct;104(2):305-16
Assay type: RNA in situ
MGI Accession ID: MGI:5469166
Gene symbol: En2
Gene name: engrailed 2
Probe: p-lambda-8SR
Probe preparation: Antisense labelled with S35 RNA
Visualized with: Autoradiography
Results
Specimen Table 1: embryonic day 12.5 (more )
Structure Level Pattern Image Note
TS20: midbrain Present
TS20: midbrain-hindbrain junction Present
TS20: hindbrain floor plate Absent There is no expression in the floor of the hindbrain.
TS20: spinal cord Absent There is no expresssion in the neural tube.
TS20: dermomyotome Absent
TS20: sclerotome Absent There is no expression in the sclerotome of developing centra.
TS20: vertebral cartilage condensation Absent There is no expression in the costal processes and neural arches.
TS20: head mesenchyme Absent There is no expression in the facial mesenchyme.
TS20: limb epithelium Absent There is no expression in the limb bud ectoderm.
TS20: body wall Absent There is no expression in thoracic body wall.
Specimen 3C/D: embryonic day 12.5 (more )
Note: sagittal section
Structure Level Pattern Image Note
TS20: midbrain Present Regionally restricted 3C/D Expression is present in discrete domains in the germinal and intermediate zones.
TS20: midbrain-hindbrain junction Present Regionally restricted 3C/D Expression is present in the roof.
TS20: hindbrain floor plate Absent 3C/D There is no expression in the germinal and intermediate zones in the anterior floor of the hindbrain.
TS20: midbrain floor plate Strong 3C/D
TS20: pons Strong 3C/D
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/18/2025
MGI 6.24 		The Jackson Laboratory