Probe:	Shh cDNA3
Probe preparation:	Antisense labelled with S35 RNA
Visualized with:	Autoradiography

Structure	Level	Pattern	Image	Note
TS20: oral epithelium	Present	Regionally restricted	1A	Expression is concentrated toward mesial part of the tooth germ (epithelial thickening).

Structure	Level	Pattern	Image	Note
TS20: oral epithelium	Present	Regionally restricted	1D	As the epithelial placode involutes into the underlying jaw mesenchyme, expression continues to be in mesial epithelial cells.

Structure	Level	Pattern	Image	Note
TS22: tooth epithelium	Present	Regionally restricted	1G	Expression is primarily in the mesial epithelial cells at this early cap stage.

Structure	Level	Pattern	Image	Note
TS24: tooth epithelium	Present	Widespread	1J	Expression is seen throughout the epithelium, although it is strongest on the mesial aspects of the developing cusps.

Structure	Level	Pattern	Image	Note
TS24: enamel organ	Present	Regionally restricted	1M	Expressed in the differentiating ameloblasts.

Structure	Level	Pattern	Image	Note
TS21: vibrissa epidermal component	Present		2A	Expressed in the epithelial whisker placode.

Structure	Level	Pattern	Image	Note
TS22: vibrissa epidermal component	Present	Regionally restricted	2D	Expressed in basal matrix cells.

Structure	Level	Pattern	Image	Note
TS24: vibrissa inner root sheath	Present		2G

Structure	Level	Pattern	Image	Note
TS22: hair follicle	Present	Regionally restricted	3A	Expressed in the epithelium.

Structure	Level	Pattern	Image	Note
TS24: hair follicle	Present	Regionally restricted	3C	Expressed in the anterior epithelial cells.

Structure	Level	Pattern	Image	Note
TS19: palatal shelf epithelium	Present	Non-Uniform	4A	Expressed throughout the maxillary oral epithelial thickening, but is concentrated mesially.

Structure	Level	Pattern	Image	Note
TS22: palatal rugae epithelium	Present		4C

Structure	Level	Pattern	Image	Note
TS19: stomach epithelium	Present	Non-Uniform	5A	High expression in the forestomach, and lower levels of expression in hindstomach.
TS19: midgut	Strong	Regionally restricted	5A	Expressed in the epithelium.
TS19: main bronchus epithelium	Strong	Uniform	5A

Structure	Level	Pattern	Image	Note
TS21: stomach epithelium	Present	Non-Uniform	5D	High expression in the forestomach, and lower levels of expression in hindstomach.

Structure	Level	Pattern	Image	Note
TS20: hindgut epithelium	Present		5G
TS20: midgut epithelium	Present		5G
TS20: caudal urogenital sinus	Present	Regionally restricted	5G	Expressed in epithelium of urethra.

Structure	Level	Pattern	Image	Note
TS20: midgut epithelium	Present		5J

Structure	Level	Pattern	Image	Note
TS22: duodenum	Present	Regionally restricted	5M	Restricted to the crypts.
TS22: midgut	Absent		5M

Structure	Level	Pattern	Image	Note
TS19: urogenital sinus	Present	Regionally restricted	6A	Expressed in the epithelium.

Structure	Level	Pattern	Image	Note
TS22: male reproductive system	Present	Regionally restricted	6C	Expressed in the epithelium of vas deferens.
TS22: bladder	Present	Regionally restricted	6C	Expressed in the epithelium.

Structure	Level	Pattern	Image	Note
TS22: lung epithelium	Present	Uniform	7A	Expressed uniformly throughout bronchiole epithelium.

Structure	Level	Pattern	Image	Note
TS22: tongue epithelium	Present	Regionally restricted	7C	Low level expression on the surface, with much higher expression in epithelial cells of the sensory papilla on the mesial portion.

Structure	Level	Pattern	Image	Note
TS20: choroid plexus	Present	Regionally restricted	7D	Expressed in epithelium of the dorsal aspect.

Structure	Level	Pattern	Image	Note
TS20: notochord	Present		7F
TS20: spinal cord floor plate	Present		7F
TS20: spinal cord lateral wall	Weak	Regionally restricted	7F	Expressed in motor neurons of the ventral horn, although expression is sporadic along the embryonic axis.

Structure	Level	Pattern	Image	Note
TS22: vestibulo-cochlear ganglion	Present		7G

Structure	Level	Pattern	Image	Note
TS22: adrenal cortex	Present		7H

Structure	Level	Pattern	Image	Note
TS20: tongue epithelium	Weak			Low level expression on the surface.

Structure	Level	Pattern	Image	Note
TS22: foregut	Present	Regionally restricted		Expressed in the epithelium.
TS22: stomach epithelium	Present	Regionally restricted		Expressed in columnar and pseudostratified cells.
TS22: ureter	Present
TS22: rectum epithelium	Absent

Structure	Level	Pattern	Image	Note
TS24: adrenal cortex	Present
TS24: hard palate palatal rugae epithelium	Present
TS24: vestibulo-cochlear ganglion	Present
TS24: tongue epithelium	Weak			Low level expression on the surface.