Probe:	Gbx2 cDNA2
Probe preparation:	Antisense labelled with S35 RNA
Visualized with:	Autoradiography
Assay notes:	Probe length was reduced by alkaline hydrolysis.

Structure	Level	Pattern	Image	Note
TS19: cerebellum primordium	Present		7A
TS19: future spinal cord	Present		7A
TS19: telencephalon	Present	Regionally restricted	7A	Expression is in the mantle part of the basal corpus striatum.
TS19: diencephalon	Present	Regionally restricted	7A	Expression is in the dorsal thalamus.

Structure	Level	Pattern	Image	Note
TS19: neural tube lateral wall	Present	Regionally restricted	7B	Labeling was restricted dorso-ventrally, the sulcus limitans demarcating a non-labeled zone.
TS19: neural tube ventricular layer	Present	Regionally restricted	7B	Expression is in two columns of labeled cells in the ventricular zone of the basal plates and a more diffuse labeling in the alar plates.
TS19: neural tube mantle layer	Present	Regionally restricted	7B	Expression is in a few cells in two columns of labeled cells in the mantle layer of the basal plates and a more diffuse labeling in the alar plates.
TS19: neural tube alar columns	Present	Regionally restricted	7B	Labeling was stronger in the boundary region between the ventricular zone and the mantle layer.
TS19: neural tube basal columns	Present	Regionally restricted	7B

Structure	Level	Pattern	Image	Note
TS21: corpus striatum	Strong		7C
TS21: thalamus	Strong		7C
TS21: hindbrain	Present		7C
TS21: medulla oblongata	Present		7C
TS21: spinal cord	Present		7C
TS21: cerebellum	Present	Regionally restricted	7C	Expression is caudal.

Structure	Level	Pattern	Image	Note
TS21: gonad	Weak		7D

Structure	Level	Pattern	Image	Note
TS28: dorsal lateral geniculate nucleus	Strong		7E
TS28: geniculate thalamic group	Strong		7E
TS28: thalamic nucleus	Strong		7E
TS28: ventral lateral geniculate nucleus	Present		7E
TS28: brainstem	Weak		7E

Structure	Level	Pattern	Image	Note
TS19: myelencephalon	Present	Regionally restricted
TS19: inner ear epithelium	Present	Regionally restricted		Expression is in the dorsal part.
TS19: oral region mesenchyme	Present	Regionally restricted		Expression is in small areas.

Structure	Level	Pattern	Image	Note
TS21: endolymphatic appendage	Present
TS21: genital tubercle	Present
TS21: semicircular canal epithelium	Present

Structure	Level	Pattern	Image	Note
TS28: amygdala	Present	Single cells
TS28: tongue epithelium	Present	Regionally restricted		Stratified epithelia.
TS28: spleen	Present	Diffuse

Structure	Level	Pattern	Image	Note
TS28: epithelium of vagina	Present
TS28: oocyte	Present