Probe:	Ser-1 cDNA
Probe preparation:	Antisense labelled with S35 RNA
Visualized with:	Autoradiography

Structure	Level	Pattern	Image	Note
TS17: hindbrain ventricular layer	Present	Regionally restricted	2A	Expressed in the ventricular region around the fourth ventricle.
TS17: 1st branchial arch	Present		2A
TS17: 2nd branchial arch	Present		2A
TS17: Rathke's pouch	Present		2A
TS17: dorsal root ganglion	Present		2A
TS17: midbrain	Present		2A
TS17: pharynx	Present		2A
TS17: telencephalon	Present		2A
TS17: future spinal cord	Present	Regionally restricted	2A	Expressed as longitudinal stripes.

Structure	Level	Pattern	Image	Note
TS17: future spinal cord	Present	Regionally restricted	2B	Expressed in four stripes.

Structure	Level	Pattern	Image	Note
TS19: midbrain	Strong	Scattered	2C	This 'salt-and-pepper' expression pattern extends across the neuroepithelium.

Structure	Level	Pattern	Image	Note
TS19: telencephalon	Strong	Scattered		This 'salt-and-pepper' expression pattern extends across the neuroepithelium.

Structure	Level	Pattern	Image	Note
TS21: midbrain	Strong	Scattered		This 'salt-and-pepper' expression pattern extends across the neuroepithelium.
TS21: telencephalon	Strong	Scattered		This 'salt-and-pepper' expression pattern extends across the neuroepithelium.

Structure	Level	Pattern	Image	Note
TS23: midbrain ventricular layer	Present	Scattered	2D
TS23: midbrain mantle layer	Ambiguous		2D	Largely devoid of expression.
TS23: midbrain marginal layer	Ambiguous		2D	Largely devoid of expression.

Structure	Level	Pattern	Image	Note
TS17: gallbladder primordium	Present	Regionally restricted	4E	Expressed in the mesenchyme.

Structure	Level	Pattern	Image	Note
TS20: lung mesenchyme	Present		4F	Expressed in the parenchyma.
TS20: lung epithelium	Absent		4F

Structure	Level	Pattern	Image	Note
TS20: vibrissa epithelium	Present	Regionally restricted		Expressed in the epithelium that invaginates the underlying condensing mesenchyme.

Structure	Level	Pattern	Image	Note
TS22: vibrissa follicle	Present		4G	Expressed in both epithelial and mesenchymal components.

Structure	Level	Pattern	Image	Note
TS22: palatal rugae epithelium	Present		4H
TS22: palatal rugae mesenchyme	Present		4H

Structure	Level	Pattern	Image	Note
TS22: pancreas epithelium	Present		4I	Mainly expressed in epithelium.

Structure	Level	Pattern	Image	Note
TS22: testis mesenchyme	Present		4J
TS22: coelomic epithelium of testis	Absent		4J

Structure	Level	Pattern	Image	Note
TS17: oral epithelium	Present		5B	Expressed in the presumptive dental epithelium.

Structure	Level	Pattern	Image	Note
TS21: tooth epithelium	Strong	Regionally restricted	5C	Expression is not observed in dental epithelial cells contacting the condensed mesenchyme.
TS21: tooth mesenchyme	Strong		5C

Structure	Level	Pattern	Image	Note
TS24: inner enamel epithelium	Absent		5D	Expression is not observed in inner enamel epithelium/preameloblasts.
TS24: dental papilla	Absent		5D
TS24: dental sac	Strong		5D
TS24: trigeminal nerve	Strong		5D

Structure	Level	Pattern	Image	Note
TS26: enamel organ	Present	Regionally restricted	5E	Expressed at low levels in the stellate reticulum and at higher levels in the stratum intermedium. No expression is observed in preameloblasts.
TS26: dental papilla	Trace		5E	Very low level.
TS26: dental sac	Present		5E
TS26: stellate reticulum	Weak		5E
TS26: stratum intermedium	Present		5E	Higher expression.

Structure	Level	Pattern	Image	Note
TS20: oral epithelium	Present		9E	Expressed in dental epithelium. Expression is absent in a layer of epithelial cells closest to the mesenchyme.
TS20: jaw primordium mesenchyme	Present		9E	Expressed in the dental mesenchyme.

Structure	Level	Pattern	Image	Note
TS23: inner enamel epithelium	Absent		9G
TS23: dental papilla	Present		9G