Gene Expression Data
Assay Details
Assay
|
Reference:
|
J:26182
Schubert FR, et al., Expression of the novel murine homeobox gene Sax-1 in the developing nervous system. Mech Dev. 1995 May;51(1):99-114
|
Assay type:
|
RNA in situ
|
MGI Accession ID:
|
MGI:5460876
|
Gene symbol:
|
Pax6
|
Gene name:
|
paired box 6
|
Probe:
|
Pax6 cDNA10
|
Probe preparation:
|
Antisense labelled with digoxigenin RNA
|
|
Results
|
Specimen
4C:
embryonic day 7.75
(more )
Note: This is a headfold stage embryo. This is a lateral view.
Specimen
4C:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 7.75
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
This is a headfold stage embryo. This is a lateral view.
|
Specimen
4F:
embryonic day 7.75
(more )
Note: This is a headfold stage embryo. This is a ventral view.
Specimen
4F:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 7.75
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
This is a headfold stage embryo. This is a ventral view.
|
Specimen
4I:
embryonic day 7.75
(more )
Note: This is a headfold stage embryo. This is a cross section.
Specimen
4I:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 7.75
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Note:
|
This is a headfold stage embryo. This is a cross section.
|
Specimen
7C:
embryonic day 10.5
(more )
Note: This is a ventral view.
Specimen
7C:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 10.5
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
This is a ventral view.
|
Structure |
Level |
Pattern |
Image |
Note |
TS17: rhombomere 1 |
Trace |
|
7C
|
Expression is mostly absent from rhombomere 1.
|
TS17: hindbrain lateral wall |
Present |
|
7C
|
Expression is present in a broad area in the medial basal plate, excluding the floor plate. It is segmentally arranged.
|
Specimen
7F:
embryonic day 10.5
(more )
Note: This is a cross section at the level of the otic vesicle.
Specimen
7F:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 10.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Gelatin
|
Note:
|
This is a cross section at the level of the otic vesicle.
|
Specimen
7I:
embryonic day 11.0
(more )
Note: This is a ventral view.
Specimen
7I:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 11.0
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
This is a ventral view.
|
Structure |
Level |
Pattern |
Image |
Note |
TS18: rhombomere 1 |
Trace |
|
7I
|
Expression is mostly absent from rhombomere 1.
|
TS18: hindbrain lateral wall |
Present |
Regionally restricted |
7I
|
Expression is present in a broad area in the medial basal plate, excluding the floor plate. It is segmentally arranged.
|
Specimen
8B:
embryonic day 11.0-11.5
(more )
Note: This is a lateral view.
Specimen
8B:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 11.0-11.5
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
This is a lateral view.
|
Structure |
Level |
Pattern |
Image |
Note |
TS19: midbrain |
Strong |
Regionally restricted |
8B
|
There is strong expression in the pretectum. Expression is also present in a single, longitudinal stripe through the caudal two-thirds of the mesencephalon.
|
TS19: diencephalon |
Strong |
Regionally restricted |
8B
|
There is strong staining in the whole epithalamus and ventral thalamus. Staining in the dorsal thalamus is weak and non-existent in the hypothalamus.
|
TS19: telencephalon |
Strong |
|
8B
|
|
TS19: hindbrain |
Present |
|
8B
|
|
Specimen
8D:
embryonic day 11.0-11.5
(more )
Specimen
8D:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 11.0-11.5
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS19: midbrain |
Strong |
Regionally restricted |
8D
|
There is strong staining in the pretectum. In the anterior it is in dispersed cells, in the posterior it is in cells of the basal plate underlying the ventricular zone.
|
TS19: diencephalon |
Strong |
Regionally restricted |
8D
|
There is strong staining in the whole epithalamus and ventral thalamus. Staining in the dorsal thalamus is weak and non-existent in the hypothalamus.
|
|