Probe:	Celsr1 cDNA
Probe preparation:	Antisense RNA

Structure	Level	Pattern	Image	Note
TS10: embryo	Present	Regionally restricted	2A	Expression is present in the distal tip of the epiblast, possibly in the anterior extremity of the primitive streak, in the vicinity where the node will form.

Structure	Level	Pattern	Image	Note
TS10: primitive streak	Present		2B
TS10: node	Present		2B	The highest levels of expression are in the ventral peri-nodal area.

Structure	Level	Pattern	Image	Note
TS11: primitive streak	Present		2C
TS11: node	Present		2C	The highest levels of expression are in the ventral peri-nodal area.

Structure	Level	Pattern	Image	Note
TS11: node	Present		2D	The highest levels of expression are in the ventral peri-nodal area.

Structure	Level	Pattern	Image	Note
TS12: primitive streak	Present		2E
TS12: embryo endoderm	Present	Regionally restricted	2E	Expression is present in a crescent of cells, where the prospective gut endoderm will commence invagination.
TS12: future brain	Present	Diffuse	2E	Expression is present in the anterior neural plate, possibly coinciding with the rostral extremity of the midbrain.

Structure	Level	Pattern	Image	Note
TS12: embryo	Present	Regionally restricted	2F	There is a sharp rostral cut-off in expression.

Structure	Level	Pattern	Image	Note
TS13: future midbrain	Present		2G
TS13: rhombomere 1	Present		2G
TS13: rhombomere 3	Present	Regionally restricted	2G	Expression is present in a thin segment.
TS13: rhombomere 5	Present	Widespread	2G	Expression is present throughout.
TS13: neural tube	Present	Widespread	2G	Expression is present along the length.

Structure	Level	Pattern	Image	Note
TS14: rhombomere 3	Present	Widespread	2H	Expression is present throughout.
TS14: rhombomere 5	Present	Widespread	2H	Expression is present throughout.
TS14: neural tube	Present		2H

Structure	Level	Pattern	Image	Note
TS15: neural tube	Present	Regionally restricted	2I	Expression is present in dorsoventrally-restricted, mediolaterally-extending "stripes."

Structure	Level	Pattern	Image	Note
TS15: neural tube	Present	Regionally restricted	2J	Expression is present in dorsoventrally-restricted, mediolaterally-extending "stripes."

Structure	Level	Pattern	Image	Note
TS15: neural tube	Present	Regionally restricted	2K	Expression is present in dorsoventrally-restricted, mediolaterally-extending "stripes."

Structure	Level	Pattern	Image	Note
TS16: neural tube roof plate	Present		2L
TS16: neural tube floor plate	Weak		2L
TS16: future brain	Present		2L

Structure	Level	Pattern	Image	Note
TS17: midbrain-hindbrain junction	Present		2M