Gene Expression Data
Assay Details
|
Assay
|
|
Reference:
|
J:75699
Albrecht AN, et al., The synpolydactyly homolog (spdh) mutation in the mouse -- a defect in patterning and growth of limb cartilage elements. Mech Dev. 2002 Mar;112(1-2):53-67
|
|
Assay type:
|
RNA in situ
|
|
MGI Accession ID:
|
MGI:5446451
|
|
Gene symbol:
|
Hoxd12
|
|
Gene name:
|
homeobox D12
|
|
Probe:
|
Hoxd12 probe9
|
|
Probe preparation:
|
Antisense labelled with digoxigenin RNA
|
|
Visualized with:
|
Alkaline phosphatase
|
|
|
Results
|
Specimen
not shown 10.5 wt:
embryonic day 10.5
(more  )
Specimen
not shown 10.5 wt:
(close  )
|
Genetic Background:
|
involves: C3H * C57BL/6
|
|
Age:
|
embryonic day 10.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
Specimen
not shown 10.5 spdh/spdh:
(close )
|
Genetic Background:
|
B6C3Fe a/a-Hoxd13spdh/J
|
|
Mutant Allele(s):
|
Hoxd13spdh/Hoxd13spdh
|
|
Age:
|
embryonic day 10.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS17: forelimb bud |
Present |
Regionally restricted |
|
Authors report normal expression domains with respect to anterior and posterior borders, but with reduced overall area of expression compared with wildtype.
|
Specimen
2 Hoxd12 11.5 wt:
embryonic day 11.5
(more )
Specimen
2 Hoxd12 11.5 wt:
(close )
|
Genetic Background:
|
involves: C3H * C57BL/6
|
|
Age:
|
embryonic day 11.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
Specimen
2 Hoxd12 11.5 spdh/spdh:
(close )
|
Genetic Background:
|
B6C3Fe a/a-Hoxd13spdh/J
|
|
Mutant Allele(s):
|
Hoxd13spdh/Hoxd13spdh
|
|
Age:
|
embryonic day 11.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
Specimen
2 Hoxd12 12.5 wt:
embryonic day 12.5
(more )
Specimen
2 Hoxd12 12.5 wt:
(close )
|
Genetic Background:
|
involves: C3H * C57BL/6
|
|
Age:
|
embryonic day 12.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
Specimen
2 Hoxd12 12.5 spdh/spdh:
(close )
|
Genetic Background:
|
B6C3Fe a/a-Hoxd13spdh/J
|
|
Mutant Allele(s):
|
Hoxd13spdh/Hoxd13spdh
|
|
Age:
|
embryonic day 12.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS20: hand |
Present |
Regionally restricted |
2_Hoxd12 12.5 spdh/spdh
|
Reduced expression domain with normal anterior and posterior borders. Expression pattern comparable to that seen at E11.5.
|
|
Analysis Tools