Probe:	MyoD probe13
Probe preparation:	Antisense RNA

Structure	Level	Pattern	Image	Note
TS15: 1st branchial arch mesenchyme	Present		1I' , 1I
TS15: dermomyotome	Present		1I

Structure	Level	Pattern	Image	Note
TS15: 1st branchial arch mesenchyme	Present		1J' , 1J
TS15: dermomyotome	Present		1J

Structure	Level	Pattern	Image	Note
TS15: 1st branchial arch mesenchyme	Present	Regionally restricted	1K' , 1K	Expression is restricted to a unilateral patch.
TS15: dermomyotome	Present		1K

Structure	Level	Pattern	Image	Note
TS17: 1st branchial arch mesenchyme	Present		5H	Expression is present in branchiomeric muscle masses.
TS17: 2nd branchial arch mesenchyme	Present		5H	Expression is present in branchiomeric muscle masses.
TS17: 3rd branchial arch mesenchyme	Present		5H	Expression is present in branchiomeric muscle masses.
TS17: 4th branchial arch mesenchyme	Present		5H	Expression is present in branchiomeric muscle masses.
TS17: 6th branchial arch mesenchyme	Present		5H	Expression is present in branchiomeric muscle masses.
TS17: branchial arch mesenchyme	Present		5H	Expression is present in branchiomeric muscle masses.
TS17: head mesenchyme	Present	Regionally restricted	5H	Expression is present in the hypoglossal cord.
TS17: myotome	Present		5H
TS17: forelimb bud mesenchyme	Present		5H	Expression is present in the muscle masses of the forelimb.

Structure	Level	Pattern	Image	Note
TS17: 1st branchial arch mesenchyme	Present		5I	Expression is present in branchiomeric muscle masses.
TS17: 2nd branchial arch mesenchyme	Present		5I	Expression is present in branchiomeric muscle masses.
TS17: 3rd branchial arch mesenchyme	Present		5I	Expression is present in branchiomeric muscle masses.
TS17: 4th branchial arch mesenchyme	Present		5I	Expression is present in branchiomeric muscle masses.
TS17: 6th branchial arch mesenchyme	Present		5I	Expression is present in branchiomeric muscle masses.
TS17: branchial arch mesenchyme	Present		5I	Expression is present in branchiomeric muscle masses.
TS17: head mesenchyme	Present	Regionally restricted	5I	Expression is present in the hypoglossal cord.
TS17: myotome	Present		5I
TS17: forelimb bud mesenchyme	Present		5I	Expression is present in the muscle masses of the forelimb.

Structure	Level	Pattern	Image	Note
TS17: 1st branchial arch mesenchyme	Absent		5J	There is no expression at sites of branchiomeric muscle formation.
TS17: branchial arch mesenchyme	Absent		5J	There is no expression at sites of branchiomeric muscle formation.
TS17: head mesenchyme	Present	Regionally restricted	5J	As in wild type, expression is present in the hypoglossal cord. However, its length is reduced owing to perturbed caudal pharyngeal morphology.
TS17: myotome	Present		5J	Expression in the mutant is the same as in wild type.
TS17: forelimb bud mesenchyme	Present		5J	As in wild type, expression is present in the muscle masses of the forelimb.

Structure	Level	Pattern	Image	Note
TS17: 1st branchial arch mesenchyme	Present	Regionally restricted	5K' , 5K	There is a patch of expression in the left mandibular arch.
TS17: head mesenchyme	Present	Regionally restricted	5K	As in wild type, expression is present in the hypoglossal cord. However, its length is reduced owing to perturbed caudal pharyngeal morphology.
TS17: myotome	Present		5K	Expression in the mutant is the same as in wild type.
TS17: forelimb bud mesenchyme	Present		5K	As in wild type, expression is present in the muscle masses of the forelimb.

Structure	Level	Pattern	Image	Note
TS17: 1st branchial arch mesenchyme	Present	Regionally restricted	5L , 5L'	There are two patches of expression in the right mandibular arch.
TS17: head mesenchyme	Present	Regionally restricted	5L	As in wild type, expression is present in the hypoglossal cord. However, its length is reduced owing to perturbed caudal pharyngeal morphology.
TS17: myotome	Present		5L	Expression in the mutant is the same as in wild type.
TS17: forelimb bud mesenchyme	Present		5L	As in wild type, expression is present in the muscle masses of the forelimb.

Structure	Level	Pattern	Image	Note
TS20: skeletal muscle	Present		6A	Expression is present in the muscles masses of the first and second arches, the caudal branchiomeric region, as well as in the trunk and the forelimb.

Structure	Level	Pattern	Image	Note
TS20: skeletal muscle	Present	Regionally restricted	6B	There is no expression in the sites of branchiomeric muscle formation, although expression is present in other muscle masses, such as those in the forelimb.

Structure	Level	Pattern	Image	Note
TS20: skeletal muscle	Present	Regionally restricted	6C	There is a patch of expression where the first arch muscles normally form, as well as expression in other muscle masses, such as those in the forelimb.

Structure	Level	Pattern	Image	Note
TS20: skeletal muscle	Present	Regionally restricted	6D	Expression is present axial and caudal branchiomeric muscle masses, such as the developing trapezius muscle.

Structure	Level	Pattern	Image	Note
TS20: skeletal muscle	Present	Regionally restricted	6E	No expression is observed in caudal branchiomeric muscle masses, although expression is present in other muscle masses.