Antibody:	anti-MEF2A
Detection system:	Secondary antibody coupled to horseradish peroxidase

Structure	Level	Pattern	Image	Note
TS13: trunk	Present	Regionally restricted	1A	Expression is in regions ventral to the somites and between the somites.
TS13: heart	Present	Punctate	1A
TS13: outflow tract	Present	Punctate	1A
TS13: primitive ventricle	Present	Punctate	1A

Structure	Level	Pattern	Image	Note
TS15: somite	Present	Regionally restricted	1E	Expression is dorso-central in the first 6 somites.
TS15: somite 05	Present	Regionally restricted	1E	Expression is dorso-central in the first 6 somites.
TS15: somite 06	Present	Regionally restricted	1E	Expression is dorso-central in the first 6 somites.
TS15: heart	Strong		1E

Structure	Level	Pattern	Image	Note
TS15: heart	Strong		1I

Structure	Level	Pattern	Image	Note
TS17: somite	Present		1M	Expression is in about 30 somites.
TS17: heart	Strong		1M

Structure	Level	Pattern	Image	Note
TS19: somite	Present		1Q
TS19: heart	Strong		1Q

Structure	Level	Pattern	Image	Note
TS14: somite 01	Present	Regionally restricted	2A	Expression is in the dorso-central region of the somites.
TS14: somite 02	Present	Regionally restricted	2A	Expression is in the dorso-central region of the somites.
TS14: head	Present	Regionally restricted	2A	Expression is in the intersomitic region.

Structure	Level	Pattern	Image	Note
TS15: somite	Present	Regionally restricted	2E	Expression is dorso-central in the first 6 somites.
TS15: somite 05	Present	Regionally restricted	2E	Expression is dorso-central in the first 6 somites.
TS15: somite 06	Present	Regionally restricted	2E	Expression is dorso-central in the first 6 somites.

Structure	Level	Pattern	Image	Note
TS15: somite	Present		2I	Expression is in the center of 16-18 somites.
TS15: tail	Strong	Regionally restricted	2I	Expression is intersomitic.

Structure	Level	Pattern	Image	Note
TS17: somite	Present		2M

Structure	Level	Pattern	Image	Note
TS15: head mesenchyme	Present	Scattered	3A	Positive cells in the forebrain and midbrain mesenchyme and surrounding the optic pit.
TS15: blood vessel	Present		3A	The authors state that expression is on structures resembling blood vessels in the midbrain.

Structure	Level	Pattern	Image	Note
TS17: diencephalon	Present	Regionally restricted	3B
TS17: telencephalon	Present	Regionally restricted	3B
TS17: blood vessel	Present	Regionally restricted	3B	Expression is in vascular structures in the head region.

Structure	Level	Pattern	Image	Note
TS15: tail	Present	Regionally restricted	3C	Expression is in the intersomitic region and in cells lining the neural tube in the caudal region.
TS15: trunk	Present	Regionally restricted	3C	Expression is in the intersomitic region and in cells lining the neural tube in the caudal region.

Structure	Level	Pattern	Image	Note
TS17: tail somite	Present	Widespread	3D
TS17: tail	Weak	Regionally restricted	3D	Expression is in the region between the somites and adjacent to the neural tube.

Structure	Level	Pattern	Image	Note
TS17: hindlimb bud mesenchyme	Present	Scattered	3E

Structure	Level	Pattern	Image	Note
TS17: hindlimb bud mesenchyme	Present	Scattered	3E inset

Structure	Level	Pattern	Image	Note
TS19: forelimb bud	Present	Regionally restricted	3F	Expression is in the proximal region.

Structure	Level	Pattern	Image	Note
TS13: blood vessel	Present		4A	Including intersomitic vessels.
TS13: trunk	Present	Regionally restricted	4A	Expression is in regions ventral to the somites and between the somites.

Structure	Level	Pattern	Image	Note
TS13: dorsal aorta	Present		4B

Structure	Level	Pattern	Image	Note
TS13: dorsal aorta	Present		4C
TS13: optic pit	Present	Regionally restricted	4C	Expression is in cells lining the optic vesicle.

Structure	Level	Pattern	Image	Note
TS13: heart	Present		4D
TS13: neural fold	Present		4D
TS13: trunk	Present	Regionally restricted	4D