Shh RNA in situ Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:42030 Hoyt PR, et al., The Evi1 proto-oncogene is required at midgestation for neural, heart, and paraxial mesenchyme development. Mech Dev. 1997 Jul;65(1-2):55-70
Assay type:	RNA in situ
MGI Accession ID:	MGI:5441510
Gene symbol:	Shh
Gene name:	sonic hedgehog

Probe:	Shh probe76
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Results

10C ©

Specimen 10C: embryonic day 10.5 (more

)

Structure	Level	Pattern	Image	Note
TS17: forelimb bud	Present	Regionally restricted	10C	Expression in the zone of polarizing activity (ZPA).
TS17: hindlimb bud	Present	Regionally restricted	10C	Expression in the zone of polarizing activity (ZPA).

10D left ©

Specimen 10D left: embryonic day 10.5; Mecom^tm1Mmor/Mecom^tm1Mmor (more

)

Structure	Level	Pattern	Image	Note
TS17: forelimb bud	Absent		10D left	No expression in the zone of polarizing activity (ZPA).

10D right ©

Specimen 10D right: embryonic day 10.5; Mecom^tm1Mmor/Mecom^tm1Mmor (more

)

Structure	Level	Pattern	Image	Note
TS17: forelimb bud	Trace	Regionally restricted	10D right	Reduced expression in the zone of polarizing activity (ZPA).
TS17: nervous system	Present		10D right	Authors report normal expression in neural tissues and notochord.
TS17: notochord	Present		10D right	Authors report normal expression in neural tissues and notochord.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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