Gene Expression Data
Assay Details
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Assay
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Reference:
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J:70830
Zelzer E, et al., Tissue specific regulation of VEGF expression during bone development requires Cbfa1/Runx2. Mech Dev. 2001 Aug;106(1-2):97-106
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:5439754
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Gene symbol:
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Pecam1
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Gene name:
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platelet/endothelial cell adhesion molecule 1
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Antibody:
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Anti-CD31
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Detection system:
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Biotinylated secondary antibody/[Strept]avidin
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Results
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Specimen
1(II)A:
embryonic day 17.5
(more  )
Specimen
1(II)A:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 17.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS25: blood vessel |
Present |
Regionally restricted |
1(II)A
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Expression was detected in blood vessels throughout the periosteum of the tibia, the surrounding tissue and in the marrow cavity. The cartilage is free of blood vessels.
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Specimen
1(II)B:
(close )
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Genetic Background:
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involves: C57BL/6
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Mutant Allele(s):
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Runx2tm1Mjo/Runx2tm1Mjo
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Age:
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embryonic day 17.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS25: blood vessel |
Present |
Regionally restricted |
1(II)B
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Expression was detected in blood vessels in the perichondrium of the tibia and the surrounding tissue, as in wild type. The entire cartilaginous shaft is devoid of blood vessels.
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Specimen
1(II)C:
(close )
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Genetic Background:
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involves: C57BL/6
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Mutant Allele(s):
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Runx2tm1Mjo/Runx2tm1Mjo
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Age:
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embryonic day 17.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS25: blood vessel |
Present |
Regionally restricted |
1(II)C
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Expression was detected in blood vessels in the perichondrium.
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Analysis Tools
