Probe:	rax cDNA5
Probe preparation:	Antisense labelled with digoxigenin RNA

Structure	Level	Pattern	Image	Note
TS9: embryo	Absent

Structure	Level	Pattern	Image	Note
TS11: future brain neural fold	Present	Regionally restricted	4A	Expression is present in the cephalic neural fold, in the area that will later become the forebrain and midbrain.

Structure	Level	Pattern	Image	Note
TS13: future prosencephalon	Strong		4B
TS13: optic pit	Present		4B	Expression is present in optic placode.

Structure	Level	Pattern	Image	Note
TS15: optic vesicle	Strong		4C

Structure	Level	Pattern	Image	Note
TS15: optic vesicle	Strong		4D
TS15: optic stalk	Present		4D
TS15: diencephalon	Present	Regionally restricted	4D	Expression is present in the ventral diencephalon.

Structure	Level	Pattern	Image	Note
TS17: optic cup	Present	Regionally restricted	4E , 4F	Expression is observed only in the retina; it is not present in the lens, optic fissure, or elsewhere in the embryo.
TS17: lens pit	Absent		4F , 4E
TS17: optic stalk	Absent		4F , 4E

Structure	Level	Pattern	Image	Note
TS19: retina	Present		4H , 4G	Expression is observed only in the retina; it is not present in the lens, optic fissure, or elsewhere in the embryo.
TS19: lens vesicle	Absent		4H , 4G
TS19: optic fissure	Absent		4H , 4G

Structure	Level	Pattern	Image	Note
TS12: neural ectoderm	Present	Regionally restricted		Expression is present in the anterior central nervous system.

Structure	Level	Pattern	Image	Note
TS13: future brain neural fold	Present	Regionally restricted	5A	Expression is present in the anterior head fold.
TS13: optic pit	Strong		5A	Expression is present in the region containing the optic evaginations.

Structure	Level	Pattern	Image	Note
TS14: optic vesicle	Present		5F	Expression is present in the optic vesicle and stalk.
TS14: future diencephalon	Present	Regionally restricted	5F	Expression is present in the ventral diencephalon.

Structure	Level	Pattern	Image	Note
TS15: diencephalon	Present	Regionally restricted	6A	Expression is present in the ventral diencephalon.
TS15: optic vesicle	Present		6A

Structure	Level	Pattern	Image	Note
TS19: neural retinal epithelium	Strong		6B
TS19: cornea	Absent		6B
TS19: lens vesicle	Absent		6B

Structure	Level	Pattern	Image	Note
TS26: neural retina	Strong		6C
TS26: retina	Present	Regionally restricted	6C	Expression is present in the neural retina. It is not present in the ganglion cell layer.
TS26: cornea	Absent		6C
TS26: lens	Absent		6C

Structure	Level	Pattern	Image	Note
TS27: retina inner nuclear layer	Present	Scattered	6G , 6D	Expression is present in the ventricular layer. (These cells are intermixed with the outer and inner nuclear layers.)
TS27: retina outer nuclear layer	Present	Scattered	6G , 6D	Expression is present in the ventricular layer. (These cells are intermixed with the outer and inner nuclear layers.)
TS27: retina ganglion cell layer	Absent		6D , 6G

Structure	Level	Pattern	Image	Note
TS28: retina outer nuclear layer	Present	Non-Uniform	6E , 6H	Expression is relatively strong near the middle of the developing retinal layers, where the most immature cells are located.

Structure	Level	Pattern	Image	Note
TS28: retina	Absent		6F , 6I