Probe:	Col10a1 probe5
Probe preparation:	Antisense labelled with digoxigenin RNA

Structure	Level	Pattern	Image	Note
TS25: humerus	Present	Regionally restricted	3C	Expression was detected in chondrocytes in the upper hypertrophic zone.

Structure	Level	Pattern	Image	Note
TS21: humerus cartilage condensation	Present	Regionally restricted	5A	Expression was detected in a small band of hypertrophic cells.

Structure	Level	Pattern	Image	Note
TS21: humerus cartilage condensation	Absent		5B

Structure	Level	Pattern	Image	Note
TS22: humerus cartilage condensation	Present	Regionally restricted	5C	Expression was detected in a small band of hypertrophic cells.

Structure	Level	Pattern	Image	Note
TS22: humerus cartilage condensation	Absent		5D

Structure	Level	Pattern	Image	Note
TS22: radius cartilage condensation	Present		5E

Structure	Level	Pattern	Image	Note
TS22: radius cartilage condensation	Weak	Regionally restricted	5F	Expression in the mutant was weak and confined to a few cells in the center of the anlage.

Structure	Level	Pattern	Image	Note
TS22: forelimb digit cartilage condensation	Present	Regionally restricted	5G	Expression was in the proximal phalanges.

Structure	Level	Pattern	Image	Note
TS22: forelimb digit cartilage condensation	Present		5H	Expression was found in the phalanges anlagen and was comparable to wild type in intensity and timing.

Structure	Level	Pattern	Image	Note
TS22: metacarpus cartilage condensation	Present

Structure	Level	Pattern	Image	Note
TS25: humerus	Present		5I

Structure	Level	Pattern	Image	Note
TS25: humerus	Absent		5J

Structure	Level	Pattern	Image	Note
TS25: radius	Present		5K

Structure	Level	Pattern	Image	Note
TS25: radius	Present		5L	By E17.5 the area of expression in the mutant was larger (than at E14.5) and similar to that of wild type mice.

Structure	Level	Pattern	Image	Note
TS25: forelimb phalanx	Present		5M

Structure	Level	Pattern	Image	Note
TS25: forelimb phalanx	Absent		5N

Structure	Level	Pattern	Image	Note
TS22: radius cartilage condensation	Weak			Expression was significantly less than wild type.
TS22: ulna cartilage condensation	Weak			Expression was significantly less than wild type.
TS22: forelimb digit cartilage condensation	Strong			Expression was similar to wild type.

Structure	Level	Pattern	Image	Note
TS25: forelimb phalanx	Absent
TS25: humerus	Absent
TS25: radius	Strong			Expression was similar to wild type.
TS25: ulna	Strong			Expression was similar to wild type.