Reporter:	lacZ
	Direct Detection
Assay notes:	The fixative was 0.2% glutaraldehyde, 2% paraformaldehyde.

Structure	Level	Pattern	Image	Note
TS19: jaw primordium	Strong
TS19: future arm	Present	Regionally restricted		Expression was detected in the presumptive shoulder girdle, and, to a lesser degree, in the condensation of the humerus.

Structure	Level	Pattern	Image	Note
TS20: humerus pre-cartilage condensation	Strong		1A
TS20: radius-ulna pre-cartilage condensation	Strong		1A
TS20: rib pre-cartilage condensation	Strong		1A
TS20: hindlimb pre-cartilage condensation	Strong	Regionally restricted	1A	Expression was strong in the tibia and fibula anlagen.
TS20: cranium	Strong	Regionally restricted	1A	Expression was strong in the developing facial bones.

Structure	Level	Pattern	Image	Note
TS21: axial skeleton	Present	Regionally restricted	1B	Expression was detected in the vertebral bodies.
TS21: forelimb digit	Present	Regionally restricted	1B	Expression was detected in the claw primordia.
TS21: forelimb digit pre-cartilage condensation	Present	Regionally restricted	1B	Expression was detected in some of the proximal phalanges of the upper limb.

Structure	Level	Pattern	Image	Note
TS22: limb	Present		1C

Structure	Level	Pattern	Image	Note
TS20: humerus pre-cartilage condensation	Present	Regionally restricted	2B	Expression was detected primarily in the perichondrium and, to a lesser degree, in chondrocytes.

Structure	Level	Pattern	Image	Note
TS20: radius-ulna pre-cartilage condensation	Present	Regionally restricted		Expression was detected primarily in the perichondrium and, to a lesser degree, in chondrocytes.

Structure	Level	Pattern	Image	Note
TS21: humerus cartilage condensation	Present	Regionally restricted	2D	Expression was diffuse in the perichondrium.

Structure	Level	Pattern	Image	Note
TS22: forelimb digit cartilage condensation	Present	Regionally restricted	2F	Expression was detected in the perichondrium. Strong expression was detected in osteoblasts.

Structure	Level	Pattern	Image	Note
TS25: humerus	Present	Regionally restricted	2H	Expression in the perichondrium was restricted to the cells directly adjacent to the cartilage. Expression was detected in the bone and in chondrocytes of the hypertrophic zone and the transition zone.

Structure	Level	Pattern	Image	Note
TS25: humerus	Present	Regionally restricted	3B	Expression was detected in chondrocytes in the upper hypertrophic zone and in calcified chondrocytes of the lower hypertrophic zone and into the transition zone. Expression was patchy in the proliferative zone. No expression was in the resting zone.

Structure	Level	Pattern	Image	Note
TS28: bone	Ambiguous			Mature bone is practically devoid of staining.
TS28: cranial suture	Present	Regionally restricted		Expression in calvarial bones is confine to the suture area, the area of new bone formation.