Probe:	TLE3 cDNA3
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS13: trunk unsegmented mesenchyme	Present	Regionally restricted		Expression is present in the posterior presomitic mesoderm subjacent to neural plate tissue at the caudal extremity of the primitive streak region.

Structure	Level	Pattern	Image	Note
TS15: tail unsegmented mesenchyme	Present		2A
TS15: head mesenchyme	Present		2A
TS15: 1st branchial arch	Present		2A
TS15: 2nd branchial arch	Present		2A
TS15: central nervous system	Weak		2A	There is low expression in the neuroepithelium.

Structure	Level	Pattern	Image	Note
TS15: somite	Present	Non-Uniform	2B	Expression is very low in recently condensed epithelial somites. It is more readily detectable in more mature somites.

Structure	Level	Pattern	Image	Note
TS17: trunk sclerotome	Present		2C	Expression is present in the ventral mesenchyme, in cells lateral to the notochord. This pattern extends along the anteroposterior axis to the level of the most occipital somites.
TS17: embryo	Present	Regionally restricted	2C	Expression is present in cell dorsolateral to the spinal cord; these are believed to be neural crest cells.
TS17: trunk dermomyotome	Present	Regionally restricted	2C	Expression is present in the dorsal epithelial cells. This pattern extends along the anteroposterior axis to the level of the most occipital somites.
TS17: notochord	Absent		2C

Structure	Level	Pattern	Image	Note
TS22: C1 vertebra	Present		3B
TS22: C2 vertebra	Present		3B
TS22: intervertebral disc	Present		3B
TS22: vertebral cartilage condensation	Present		3B
TS22: central nervous system	Strong		3B

Structure	Level	Pattern	Image	Note
TS24: intervertebral disc	Present		3D
TS24: vertebra	Present		3D
TS24: dorsal root ganglion	Present	Non-Uniform	3D	Expression is found predominantly in cells located around the periphery.
TS24: skin	Present		3D

Structure	Level	Pattern	Image	Note
TS20: telencephalon	Present		5B	Expression is present in the neuroepithelium.
TS20: diencephalon	Present		5B
TS20: head mesenchyme	Present		5B
TS20: medulla oblongata alar plate	Present		5B
TS20: skin	Present		5B	Expression is present in the surface ectoderm.

Structure	Level	Pattern	Image	Note
TS22: telencephalon	Present		5D	Expression is present in the neuroepithelium.
TS22: corpus striatum	Present		5D
TS22: cerebellum	Present		5D
TS22: diencephalon	Present		5D
TS22: metencephalon	Present		5D
TS22: medulla oblongata alar plate	Present		5D

Structure	Level	Pattern	Image	Note
TS24: cerebral cortex intermediate zone	Present		5F
TS24: cerebral cortex subventricular zone	Present		5F
TS24: cerebral cortex ventricular layer	Present		5F
TS24: cortical plate	Present	Non-Uniform	5F	Expression is present in more external layers of cells in the cortical plate.
TS24: skin	Present		5F