Gene Expression Data
Assay Details
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Assay
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Reference:
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J:96463
Moraes F, et al., Tbx1 is required for proper neural crest migration and to stabilize spatial patterns during middle and inner ear development. Mech Dev. 2005 Feb;122(2):199-212
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:5435577
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Gene symbol:
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Hmx3
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Gene name:
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H6 homeobox 3
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Probe:
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Nkx5.1 probe1
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Assay notes:
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After whole-mount staining, embryos were post-fixed in 4% paraformaldehyde/0.2% glutaraldehyde before sectioning.
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Results
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Specimen
4I:
embryonic day 9.5
(more  )
Specimen
4I:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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whole mount
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: otocyst |
Present |
Regionally restricted |
4I
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Expression was detected in the latero-dorsal areas of the otocyst.
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Specimen
4J:
embryonic day 9.5
(more )
Specimen
4J:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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Other - See Notes
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Embedding:
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Gelatin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: otocyst |
Present |
Regionally restricted |
4J
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Expression was detected in the latero-dorsal areas of the otocyst.
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Specimen
4K:
(close )
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Genetic Background:
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involves: 129 * C57BL/6
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Mutant Allele(s):
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Tbx1tm1Pa/Tbx1tm1Pa
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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whole mount
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: otocyst |
Present |
Regionally restricted |
4K
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Expression was detected in the latero-dorsal areas of the otocyst as in wild type.
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Specimen
4L:
(close )
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Genetic Background:
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involves: 129 * C57BL/6
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Mutant Allele(s):
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Tbx1tm1Pa/Tbx1tm1Pa
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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Other - See Notes
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Embedding:
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Gelatin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: otocyst |
Present |
Regionally restricted |
4L
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Expression was detected in the latero-dorsal areas of the otocyst as in wild type.
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Specimen
5I:
embryonic day 10.5
(more )
Specimen
5I:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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whole mount
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: otocyst |
Present |
Regionally restricted |
5I
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Expression was detected in the lateral and dorsal areas of the otic vesicle.
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Specimen
5J:
embryonic day 10.5
(more )
Specimen
5J:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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Other - See Notes
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Embedding:
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Gelatin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: otocyst |
Present |
Regionally restricted |
5J
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Expression was detected in the lateral and dorsal areas of the otic vesicle.
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Specimen
5K:
(close )
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Genetic Background:
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involves: 129 * C57BL/6
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Mutant Allele(s):
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Tbx1tm1Pa/Tbx1tm1Pa
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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whole mount
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: otocyst |
Present |
Regionally restricted |
5K
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Expression was extended ventrally in the lateral wall and lost intensity in the mutant.
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Specimen
5L:
(close )
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Genetic Background:
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involves: 129 * C57BL/6
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Mutant Allele(s):
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Tbx1tm1Pa/Tbx1tm1Pa
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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Other - See Notes
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Embedding:
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Gelatin
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS17: otocyst |
Present |
Regionally restricted |
5L
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Expression was extended ventrally in the lateral wall and lost intensity in the mutant.
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Analysis Tools