Gene Expression Data
Assay Details
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Assay
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Reference:
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J:96463
Moraes F, et al., Tbx1 is required for proper neural crest migration and to stabilize spatial patterns during middle and inner ear development. Mech Dev. 2005 Feb;122(2):199-212
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:5435406
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Gene symbol:
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Bmp4
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Gene name:
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bone morphogenetic protein 4
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Probe:
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Bmp4 probe35
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Assay notes:
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After whole-mount staining, embryos were post-fixed in 4% paraformaldehyde/0.2% glutaraldehyde before sectioning.
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Results
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Specimen
3I:
embryonic day 15.5
(more  )
Specimen
3I:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 15.5
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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Other - See Notes
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Embedding:
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Gelatin
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Specimen
3N:
(close )
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Genetic Background:
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involves: 129 * C57BL/6
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Mutant Allele(s):
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Tbx1tm1Pa/Tbx1tm1Pa
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Age:
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embryonic day 15.5
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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Other - See Notes
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Embedding:
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Gelatin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS23: inner ear epithelium |
Present |
Regionally restricted |
3N
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Expression was detected in the patch-like structure observed in the inner ears of mutant embryos. The expression domain was not of the pattern of maculae, cristae or the organ of Corti.
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Specimen
5M:
embryonic day 10.5
(more )
Specimen
5M:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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whole mount
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: otocyst |
Present |
Regionally restricted |
5M
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Expression was localized in two domains - one in the posterior wall and one in the lateral wall.
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Specimen
5N:
embryonic day 10.5
(more )
Specimen
5N:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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Other - See Notes
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Embedding:
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Gelatin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: otocyst |
Present |
Regionally restricted |
5N
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Expression was localized in two domains - one in the posterior wall and one in the lateral wall.
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Specimen
5O:
(close )
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Genetic Background:
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involves: 129 * C57BL/6
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Mutant Allele(s):
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Tbx1tm1Pa/Tbx1tm1Pa
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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whole mount
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: otocyst |
Present |
Regionally restricted |
5O
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The posterior expression domain was downregulated in most, but not all, mutant embryos. The lateral domain was still detectable, but its position was different and was wider along the dorso-ventral axis in the mutant compared to the wild type.
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Specimen
5P:
(close )
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Genetic Background:
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involves: 129 * C57BL/6
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Mutant Allele(s):
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Tbx1tm1Pa/Tbx1tm1Pa
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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Other - See Notes
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Embedding:
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Gelatin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: otocyst |
Present |
Regionally restricted |
5P
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The posterior expression domain was downregulated in most, but not all, mutant embryos. The lateral domain was still detectable, but its position was different and was wider along the dorso-ventral axis in the mutant compared to the wild type.
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Analysis Tools