Probe:	Raldh3 cDNA3
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS13: surface ectoderm	Present	Regionally restricted	6Aa	Expression is in the surface ectoderm of the prospective eye field.

Structure	Level	Pattern	Image	Note
TS14: eye	Present		6Ab
TS14: surface ectoderm	Present	Regionally restricted	6Ab	Expression is in the surface ectoderm in the ventral head.

Structure	Level	Pattern	Image	Note
TS15: lens placode	Strong		6Ac
TS15: olfactory placode	Strong		6Ac
TS15: spinal ganglion	Strong		6Ac
TS15: surface ectoderm	Strong	Regionally restricted	6Ac	Expression is in the surface ectoderm in the ventral head.
TS15: future spinal cord	Present	Regionally restricted	6Ac	Expression is in the posterior spinal cord.

Structure	Level	Pattern	Image	Note
TS15: surface ectoderm	Present	Regionally restricted	6Ad	Expression is in the surface ectoderm in the ventral head.
TS15: optic cup	Present	Regionally restricted	6Ad	Expression is dorsal.

Structure	Level	Pattern	Image	Note
TS16: lens pit	Present		6Ae
TS16: nasal pit	Present		6Ae

Structure	Level	Pattern	Image	Note
TS16: lens pit	Strong		6Af

Structure	Level	Pattern	Image	Note
TS19: retina	Present	Regionally restricted	6Ag	Expression is ventral.
TS19: nasal pit	Present		6Ag
TS19: mandibular process	Present	Regionally restricted	6Ag	Expression is at the edge.

Structure	Level	Pattern	Image	Note
TS19: retina	Present	Regionally restricted	6Ah	Expression is ventral, faint in the dorsal area.
TS19: lens vesicle	Absent		6Ah

Structure	Level	Pattern	Image	Note
TS22: retina	Weak	Regionally restricted	6Ai	Expression is ventral.