Probe:	pExpress-1-Bmp2
Probe preparation:	Antisense labelled with digoxigenin RNA

Structure	Level	Pattern	Image	Note
TS10: allantois	Present		1A1
TS10: yolk sac	Present		1A1
TS10: embryo	Present	Regionally restricted	1A1	Expression is in the pre-cardiac crescent, just anterior to the anterior intestinal portal and is absent in the medial, open gut region of the embryo, which includes pre-somitic mesoderm, endoderm, and neural tissues.

Structure	Level	Pattern	Image	Note
TS12: allantois	Strong		1A2
TS12: lateral plate mesoderm	Strong		1A2
TS12: primitive heart tube	Strong		1A2
TS12: sinus venosus	Strong		1A2
TS12: foregut diverticulum	Strong	Regionally restricted	1A2	Expression is in the constricting anterior intestinal portal.
TS12: future brain neural fold	Strong	Regionally restricted	1A2	Expression is in the dorsal most tip of the rostroanterior neural folds.
TS12: neural fold	Strong	Regionally restricted	1A2	Expression is in the midline fusion point of the neural folds spanning the length of the embryo.

Structure	Level	Pattern	Image	Note
TS13: sinus venosus	Strong		1A3
TS13: neural tube	Strong	Regionally restricted	1A3	Expression is in the fusing dorsal neural tube.
TS13: heart	Strong	Regionally restricted	1A3	Expression is in the region that joins the left ventricle and the atria.
TS13: pericardial component	Strong		1A3
TS13: future brain neural fold	Present	Regionally restricted	1A3	Expression is in the region of the midline fusion of the anterior neural folds.

Structure	Level	Pattern	Image	Note
TS14: atrioventricular canal	Strong		1A4
TS14: hepatic primordium	Strong		1A4
TS14: dorsal aorta	Present	Regionally restricted	1A4	Expression is rostral.
TS14: pronephros	Present	Regionally restricted	1A4	Expression is in the anterior tip of the developing mesonephros.
TS14: prosencephalon	Weak	Regionally restricted	1A4	Expression is at the midline seam of the telencephalon.
TS14: yolk sac	Weak	Regionally restricted	1A4	Expression is in the constricting base of the yolk sac.
TS14: branchial arch	Weak		1A4
TS14: head	Weak		1A4
TS14: mesenchyme derived from lateral plate	Weak		1A4
TS14: neural tube	Weak	Regionally restricted	1A4	Expression is dorsal.

Structure	Level	Pattern	Image	Note
TS15: forelimb bud presumptive apical ectodermal ridge	Present		1A5
TS15: atrioventricular canal	Strong		1A5
TS15: branchial arch	Present	Regionally restricted	1A5	Expression is prominent in subpopulations of cells between the branchial arches.

Structure	Level	Pattern	Image	Note
TS17: gut	Weak		1A6
TS17: head	Weak		1A6
TS17: tail	Weak		1A6
TS17: yolk sac	Present		1A6
TS17: forelimb bud ectoderm	Present	Regionally restricted	1A6	Expression is in an ectodermal patch on the posterior aspect of the forelimbs.
TS17: apical ectodermal ridge	Strong		1A6
TS17: somite	Present	Regionally restricted	1A6	Expression in the ventromedial somites increases, especially in the anterior trunk.

Structure	Level	Pattern	Image	Note
TS13: neural tube	Strong	Regionally restricted	2A1	Expression is in the fusing dorsal neural tube.

Structure	Level	Pattern	Image	Note
TS14: prosencephalon	Weak	Regionally restricted	2A2	Expression is at the midline seam of the telencephalon.

Structure	Level	Pattern	Image	Note
TS14: forelimb bud ectoderm	Present	Regionally restricted	2A3	Expression is in the apical ectodermal ridge.

Structure	Level	Pattern	Image	Note
TS17: gut	Weak		2A4
TS17: head	Weak		2A4
TS17: tail	Weak		2A4

Structure	Level	Pattern	Image	Note
TS17: branchial arch	Strong		2A5
TS17: otocyst	Strong		2A5

Structure	Level	Pattern	Image	Note
TS17: atrioventricular canal	Strong		2A5 inset

Structure	Level	Pattern	Image	Note
TS17: somite	Present	Regionally restricted	2A6	Expression in the ventromedial somites increases, especially in the anterior trunk.

Structure	Level	Pattern	Image	Note
TS13: neural tube	Strong	Regionally restricted	3A1	Expression is in the fusing dorsal neural tube.

Structure	Level	Pattern	Image	Note
TS14: prosencephalon	Weak	Regionally restricted	3A2	Expression is at the midline seam of the telencephalon.

Structure	Level	Pattern	Image	Note
TS14: forelimb bud ectoderm	Present	Regionally restricted	3A3	Expression is in the apical ectodermal ridge.

Structure	Level	Pattern	Image	Note
TS17: gut	Weak		3A4

Structure	Level	Pattern	Image	Note
TS17: lung	Absent		3A5

Structure	Level	Pattern	Image	Note
TS13: neural fold	Strong
TS13: future hindbrain	Present
TS13: future midbrain	Present
TS13: heart	Present
TS13: future prosencephalon	Weak	Regionally restricted		Expression is in telencephalon.
TS13: future brain	Weak	Regionally restricted		Expression is in the seam.
TS13: somite	Weak

Structure	Level	Pattern	Image	Note
TS14: limb	Moderate
TS14: future midbrain	Present
TS14: heart	Present
TS14: future brain	Weak	Regionally restricted		Expression is in the seam.
TS14: prosencephalon	Weak	Regionally restricted		Expression is in telencephalon.
TS14: future hindbrain	Weak
TS14: somite	Weak

Structure	Level	Pattern	Image	Note
TS17: limb	Strong
TS17: heart	Moderate
TS17: somite	Present
TS17: telencephalon	Present
TS17: brain	Weak	Regionally restricted		Expression is in the seam.
TS17: hindbrain	Weak
TS17: midbrain	Weak
TS17: lung	Absent
TS17: stomach	Absent