Gene Expression Data
Assay Details
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Assay
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Reference:
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J:179032
Sharlin DS, et al., Developmental and cell-specific expression of thyroid hormone transporters in the mouse cochlea. Endocrinology. 2011 Dec;152(12):5053-64
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:5311329
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Gene symbol:
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Slc16a2
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Gene name:
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solute carrier family 16 (monocarboxylic acid transporters), member 2
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Antibody:
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anti-Mct8
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Detection system:
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Secondary antibody coupled to Alexa Fluor
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Assay notes:
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The antibody used in this assay produced nonspecific nuclear signal in inner hair cells and spiral ganglion cell.
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Results
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Specimen
4B:
postnatal day 3
(more  )
Note: Double labeled: red - Slc16a2; green - Pecam1 (assay MGI:5311190).
Specimen
4B:
(close  )
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Genetic Background:
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C57BL/6J
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Age:
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postnatal day 3
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: red - Slc16a2; green - Pecam1 (assay MGI:5311190).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS27: organ of Corti |
Present |
Regionally restricted |
4B
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Signal detected in the columnar cells of the greater epithelial ridge.
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| TS27: capillary |
Absent |
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4B
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No signal in CD31-positive capillaries in the spiral organ.
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Specimen
4D:
postnatal day 15
(more )
Note: Double labeled: red - Slc16a2; green - Pecam1 (assay MGI:5311190).
Specimen
4D:
(close )
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Genetic Background:
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C57BL/6J
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Age:
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postnatal day 15
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: red - Slc16a2; green - Pecam1 (assay MGI:5311190).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: cochlear duct |
Present |
Regionally restricted |
4D
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Signal detected in the cell membrane of the tympanic border cells.
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| TS28: blood vessel |
Absent |
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4D
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No signal in CD31-positive vessel in the spiral organ.
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Specimen
4F:
postnatal day 15
(more )
Note: Double labeled: red - Slc16a2; green - Pecam1 (assay MGI:5311190).
Specimen
4F:
(close )
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Genetic Background:
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C57BL/6J
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Age:
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postnatal day 15
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: red - Slc16a2; green - Pecam1 (assay MGI:5311190).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: capillary |
Weak |
Regionally restricted |
4F
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Weak signal was detected in abluminal side of CD31-positive capillaries in the stria vascularis, but not in the spiral ligmant.
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| TS28: spiral ligament |
Present |
Regionally restricted |
4F
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Signal was detected in spiral ligament fibrocytes.
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Specimen
S1C Mct8y/+ top:
postnatal day 7
(more )
Specimen
S1C Mct8 top:
(close )
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Genetic Background:
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Not Specified
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Age:
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postnatal day 7
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Sex:
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Male
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: cochlear duct |
Present |
Regionally restricted |
S1C Mct8y/+ top
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Signal was detected in tympanic border cells.
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| TS28: organ of Corti |
Present |
Regionally restricted |
S1C Mct8y/+ top
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Signal was detected in the greater epithelial ridge.
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Specimen
S1C Mct8y/+ bottom:
postnatal day 7
(more )
Specimen
S1C Mct8 bottom:
(close )
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Genetic Background:
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Not Specified
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Age:
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postnatal day 7
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Sex:
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Male
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
S1C Mct8 top:
(close )
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Genetic Background:
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either: (involves: C57BL/6N) or (involves: NMRI)
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Mutant Allele(s):
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Slc16a2tm1Dgen/Y
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Age:
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postnatal day 7
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Sex:
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Male
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: organ of Corti |
Absent |
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S1C Mct8y/- top
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Signal was not detected in the greater epithelial ridge.
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| TS28: cochlear duct |
Absent |
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S1C Mct8y/- top
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Signal was not detected in tympanic border cells.
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Specimen
S1C Mct8y/- bottom:
postnatal day 7; Slc16a2tm1Dgen/Y
(more )
Specimen
S1C Mct8 bottom:
(close )
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Genetic Background:
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either: (involves: C57BL/6N) or (involves: NMRI)
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Mutant Allele(s):
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Slc16a2tm1Dgen/Y
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Age:
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postnatal day 7
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Sex:
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Male
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
not shown:
postnatal day 15
(more )
Specimen
not shown:
(close )
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Genetic Background:
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Not Specified
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Age:
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postnatal day 15
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Sex:
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Male
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
not shown:
(close )
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Genetic Background:
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either: (involves: C57BL/6N) or (involves: NMRI)
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Mutant Allele(s):
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Slc16a2tm1Dgen/Y
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Age:
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postnatal day 15
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Sex:
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Male
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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