Gene Expression Data
Assay Details
Assay
|
Reference:
|
J:80025
Wallis D, et al., The zinc finger transcription factor Gfi1, implicated in lymphomagenesis, is required for inner ear hair cell differentiation and survival. Development. 2003 Jan;130(1):221-32
|
Assay type:
|
RNA in situ
|
MGI Accession ID:
|
MGI:5302554
|
Gene symbol:
|
Pou4f3
|
Gene name:
|
POU domain, class 4, transcription factor 3
|
Probe:
|
Brn3c probe1
|
Probe preparation:
|
Antisense labelled with digoxigenin RNA
|
Visualized with:
|
Alkaline phosphatase
|
|
Results
|
Specimen
3I:
embryonic day 18.5
(more )
Specimen
3I:
(close )
Genetic Background:
|
involves: 129S1/Sv * C57BL/6J
|
Age:
|
embryonic day 18.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS26: organ of Corti |
Present |
Regionally restricted |
3I
|
Expression is present in the hair cells.
|
Specimen
3J:
(close )
Genetic Background:
|
involves: 129S1/Sv * C57BL/6J
|
Mutant Allele(s):
|
Gfi1tm1Sho/Gfi1tm1Sho
|
Age:
|
embryonic day 18.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS26: organ of Corti |
Present |
Regionally restricted |
3J
|
Expression is present in the hair cells; they are disorganized in the mutant and their numbers appear to be reduced.
|
|