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Gene Expression Data
Assay Details
Assay
Reference: J:80020 Marcil A, et al., Pitx1 and Pitx2 are required for development of hindlimb buds. Development. 2003 Jan;130(1):45-55
Assay type: RNA in situ
MGI Accession ID: MGI:5297966
Gene symbol: Fgf8
Gene name: fibroblast growth factor 8
Probe: Fgf8 probe21
Probe preparation: Antisense labelled with digoxigenin RNA
Results
Specimen 4B left: embryonic day 10.5 (more )
Note: This specimen is at the 39-somite stage.
Structure Level Pattern Image Note
TS17: hindlimb bud apical ectodermal ridge Present 4B left
Specimen 4B middle: embryonic day 10.5; Pitx1tm1Jdr/Pitx1tm1Jdr (more )
Note: This specimen is at the 40-somite stage.
Structure Level Pattern Image Note
TS18: hindlimb bud apical ectodermal ridge Present 4B middle Expression appears to be similar to that seen in wild-type.
Specimen 4B right: embryonic day 10.5; Pitx1tm1Jdr/Pitx1tm1Jdr, Pitx2tm2Sac/Pitx2+ (more )
Note: This specimen is at the 38-somite stage.
Structure Level Pattern Image Note
TS17: hindlimb bud apical ectodermal ridge Present 4B right Expression appears to be similar to that seen in wild-type.
Specimen 4H left 28so: embryonic day 9.5 (more )
Note: This specimen is at the 28-somite stage.
Structure Level Pattern Image Note
TS15: hindlimb ridge ectoderm Present Regionally restricted 4H left 28so Expression is present in the apical ectodermal ridge.
Specimen 4H middle: embryonic day 9.5; Pitx1tm1Jdr/Pitx1tm1Jdr (more )
Note: This specimen is at the 29-somite stage.
Structure Level Pattern Image Note
TS15: hindlimb ridge ectoderm Present Regionally restricted 4H middle Expression is present in the apical ectodermal ridge; its level is slightly reduced in the mutant.
Specimen 4H right 29so: embryonic day 9.5; Pitx1tm1Jdr/Pitx1tm1Jdr, Pitx2tm2Sac/Pitx2+ (more )
Note: This specimen is at the 29-somite stage.
Structure Level Pattern Image Note
TS15: hindlimb ridge ectoderm Absent 4H right 29so Expression is delayed in the mutant relative to wild-type.
Specimen 4H left 30so: embryonic day 9.5 (more )
Note: This specimen is at the 30-somite stage.
Structure Level Pattern Image Note
TS16: hindlimb bud ectoderm Present Regionally restricted 4H left 30so Expression is present in the apical ectodermal ridge.
Specimen 4H right 30 so: embryonic day 9.5; Pitx1tm1Jdr/Pitx1tm1Jdr, Pitx2tm2Sac/Pitx2+ (more )
Note: This specimen is at the 30-somite stage.
Structure Level Pattern Image Note
TS16: hindlimb bud ectoderm Present Regionally restricted 4H right 30so Expression is present in the apical ectodermal ridge; its onset is delayed in the mutant compared to wild-type.
Specimen 4H left 32so: embryonic day 10.0; Pitx1tm1Jdr/Pitx1tm1Jdr (more )
Note: This specimen is at the 32-somite stage.
Structure Level Pattern Image Note
TS16: hindlimb bud ectoderm Present Regionally restricted 4H left 32so Expression is present in the apical ectodermal ridge.
Specimen 4H right 32so: embryonic day 10.0; Pitx1tm1Jdr/Pitx1tm1Jdr, Pitx2tm1Sac/Pitx2+ (more )
Note: This specimen is at the 32-somite stage.
Structure Level Pattern Image Note
TS16: hindlimb bud ectoderm Present Regionally restricted 4H right 32so Expression is present in the apical ectodermal ridge; its onset is delayed in the mutant compared to wild-type.
Specimen 6D top left: embryonic day 12.5; Pitx1tm1Jdr/Pitx1+, Pitx2tm2Sac/Pitx2tm2Sac (more )
Structure Level Pattern Image Note
TS20: hand epithelium Present 6D top left Expression is present in the apical ectodermal ridge.
Specimen 6D top right: embryonic day 12.5; Pitx1tm1Jdr/Pitx1tm1Jdr, Pitx2tm2Sac/Pitx2tm2Sac (more )
Structure Level Pattern Image Note
TS20: hand epithelium Present 6D top right Expression is present in the apical ectodermal ridge. Expression in the mutant appears to be similar to that seen in wild type.
Specimen 6D bottom left: embryonic day 12.5; Pitx1tm1Jdr/Pitx1+, Pitx2tm2Sac/Pitx2tm2Sac (more )
Structure Level Pattern Image Note
TS20: foot epithelium Present 6D bottom left Expression is present in the apical ectodermal ridge.
Specimen 6D bottom right: embryonic day 12.5; Pitx1tm1Jdr/Pitx1tm1Jdr, Pitx2tm2Sac/Pitx2tm2Sac (more )
Structure Level Pattern Image Note
TS20: foot epithelium Present Regionally restricted 6D bottom right There is some expression present in the left (but not right) apical ectodermal ridge. (The left bud is larger than the right one, but both hindlimb buds are smaller in the mutant than in wild-type.)
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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12/30/2025
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