Probe:	Wnt3 cDNA2
Probe preparation:	Antisense RNA
Visualized with:	Autoradiography

Structure	Level	Pattern	Image	Note
TS13: future hindbrain	Present	Non-Uniform	1A/B	Expression is present in a restricted area of the presumptive myelencephalon. A low level of expression is also detected in the presumptive mesencephalon.

Structure	Level	Pattern	Image	Note
TS14: surface ectoderm	Present		1C/D
TS14: future hindbrain	Present	Regionally restricted	1C/D	Expression is present in the dorsal region of the myelencephalon.
TS14: future diencephalon	Weak		1C/D

Structure	Level	Pattern	Image	Note
TS14: future hindbrain	Present	Regionally restricted		Expression is present in the dorsal area of the mesencephalon.

Structure	Level	Pattern	Image	Note
TS14: future diencephalon	Present	Regionally restricted	2A , 2B/C	Expression is present in a restricted area that has sharp anterior and posterior boundaries. It may correspond to the presumptive dorsal thalamus.

Structure	Level	Pattern	Image	Note
TS15: future midbrain	Present	Regionally restricted	2D/E	Expression is present in the dorsal areas.
TS15: future spinal cord	Present	Regionally restricted	2D/E	Expression is present in the dorsal areas.
TS15: future hindbrain	Present	Regionally restricted	2D/E , 2F	Expression is present in the dorsal areas of the metencephalon and anterior myelencephalon.
TS15: limb ectoderm	Present		2D/E	Expression is present in the surface ectoderm.

Structure	Level	Pattern	Image	Note
TS16: diencephalon	Present	Regionally restricted	3A	Expression is present from the diencephalon along the dorsal midline of the mesencephalon, metencephalon, myelencephaon, and spinal cord.
TS16: future hindbrain	Present	Regionally restricted	3A	Expression is present from the diencephalon along the dorsal midline of the mesencephalon, metencephalon, myelencephaon, and spinal cord.
TS16: future midbrain	Present	Regionally restricted	3A	Expression is present from the diencephalon along the dorsal midline of the mesencephalon, metencephalon, myelencephaon, and spinal cord.
TS16: neural tube	Present	Regionally restricted	3A	Expression is present from the diencephalon along the dorsal midline of the mesencephalon, metencephalon, myelencephaon, and spinal cord.

Structure	Level	Pattern	Image	Note
TS16: diencephalon	Present	Regionally restricted	3E/F , 3C	Expression is restricted to the presumptive dorsal thalamus. There are two levels of expression; the highest levels were observed at the most anterior border.

Structure	Level	Pattern	Image	Note
TS19: diencephalon	Present	Regionally restricted		Expression is present in the presumptive dorsal thalamus.

Structure	Level	Pattern	Image	Note
TS20: thalamus	Present	Regionally restricted	4A/B	Expression is present in the dorsal thalamus.
TS20: midbrain	Present		4A/B	Areas of expression include the tectum.
TS20: cerebellum primordium	Present		4A/B
TS20: skin	Present		4A/B	Expression is present in the surface ectoderm.
TS20: diencephalon	Present	Regionally restricted	4A/B	There is expression in cells immediately adjacent to the anterior diencephalic furrow.

Structure	Level	Pattern	Image	Note
TS20: diencephalon	Present	Regionally restricted	4D	There are sharp boundaries of expression.

Structure	Level	Pattern	Image	Note
TS21: thalamus	Present	Regionally restricted	5A	Expression is present in the dorsal thalamus.
TS21: medulla oblongata	Present		5A
TS21: trigeminal ganglion	Present		5A

Structure	Level	Pattern	Image	Note
TS21: cerebellum	Present			Expression levels are starting to decrease from those seen earlier.
TS21: midbrain	Present			Expression levels are starting to decrease from those seen earlier.
TS21: spinal cord lateral wall	Present	Regionally restricted		Expression levels at the dorsal midline are starting to decrease from those seen earlier.

Structure	Level	Pattern	Image	Note
TS21: thalamus	Present	Regionally restricted	5B	Expression is present in the internal and external germinal layers of the dorsal thalamus.

Structure	Level	Pattern	Image	Note
TS22: diencephalon	Present

Structure	Level	Pattern	Image	Note
TS23: thalamus	Present	Regionally restricted	5C	Expression is present in the dorsal thalamus.

Structure	Level	Pattern	Image	Note
TS23: cerebellum	Weak
TS23: tectum	Weak

Structure	Level	Pattern	Image	Note
TS24: diencephalon	Present		5E
TS24: thalamus	Present	Regionally restricted	5E	Expression is present in the dorsal thalamus, in different nuclei such as the corpus geniculatum laterale pars dorsale and the nucleus medialis dorsalis.

Structure	Level	Pattern	Image	Note
TS28: lateral thalamic group	Present		5F	Expression is present in several thalamic nuclei: lateralis, medialis pars lateralis and medialis, ventralis pars lateralis, paraventricularis, rhomboideus and reuniens.
TS28: medial thalamic group	Present		5F	Expression is present in several thalamic nuclei: lateralis, medialis pars lateralis and medialis, ventralis pars lateralis, paraventricularis, rhomboideus and reuniens.
TS28: thalamic nucleus	Present		5F	Expression is present in several thalamic nuclei: lateralis, medialis pars lateralis and medialis, ventralis pars lateralis, paraventricularis, rhomboideus and reuniens.
TS28: lateral habenular nucleus	Present		5F
TS28: medial habenular nucleus	Present		5F