Gene Expression Data
Assay Details
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Assay
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Reference:
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J:42455
Dunwoodie SL, et al., Mouse Dll3: a novel divergent Delta gene which may complement the function of other Delta homologues during early pattern formation in the mouse embryo. Development. 1997 Aug;124(16):3065-76
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:5295479
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Gene symbol:
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Dll3
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Gene name:
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delta like canonical Notch ligand 3
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Probe:
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Dll3 cDNA2
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Assay notes:
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It is possible that Probe Dll3 cDNA3 (MGI:2446514) was used for this assay. The probe is also possible being labelled with fluorescein, and detected by alkaline phosphatase-conjugated anti-fluorescein antibody.
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Results
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Specimen
8A:
embryonic day 12.5
(more  )
Specimen
8A:
(close  )
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Genetic Background:
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involves: C57BL/6 * DBA
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
8C:
embryonic day 10.5
(more )
Note: Double labeled: brown - Dll3; blue - Dll1 (assay MGI:5295481).
Specimen
8C:
(close )
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Genetic Background:
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involves: C57BL/6 * DBA
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: brown - Dll3; blue - Dll1 (assay MGI:5295481).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: neural tube lateral wall |
Present |
Regionally restricted |
8C
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Expression in the basal surface of the ventricular zone along its dorsoventral extent, lateral to Dll1 expression. Overlap of expression normally corresponds with the expression of Dll3 extending towards the lumen to the Dll1 domain.
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Specimen
8D:
embryonic day 12.5
(more )
Note: Double labeled: blue - Dll3; brown - Dll1 (assay MGI:5295481).
Specimen
8D:
(close )
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Genetic Background:
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involves: C57BL/6 * DBA
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: blue - Dll3; brown - Dll1 (assay MGI:5295481).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: hindbrain ventricular layer |
Present |
Regionally restricted |
8D
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Expression in the ventricular zone adjacent to the basal surface in domains spreading medial to lateral, coincide or separate from Dll1 expression.
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Specimen
8E:
embryonic day 12.5
(more )
Note: Double labeled: blue - Dll3; brown - Dll1 (assay MGI:5295481).
Specimen
8E:
(close )
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Genetic Background:
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involves: C57BL/6 * DBA
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: blue - Dll3; brown - Dll1 (assay MGI:5295481).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: hindbrain ventricular layer |
Present |
Regionally restricted |
8E
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Expression in the ventricular zone adjacent to the basal surface in domains spreading medial to lateral, coincide or separate from Dll1 expression.
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Specimen
8F:
embryonic day 8.5
(more )
Note: This is a 10-somite embryo. Double labeled: brown - Dll3; blue - Dll1 (assay MGI:5295481).
Specimen
8F:
(close )
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Genetic Background:
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involves: C57BL/6 * DBA
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Age:
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embryonic day 8.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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This is a 10-somite embryo. Double labeled: brown - Dll3; blue - Dll1 (assay MGI:5295481).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS13: future midbrain |
Present |
Regionally restricted |
8F
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Colocalise with Dll1 in the dorsal midbrain.
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Specimen
8G left:
embryonic day 9.5
(more )
Specimen
8G left:
(close )
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Genetic Background:
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involves: C57BL/6 * DBA
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: future forebrain |
Present |
Regionally restricted |
8G left
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Expression is restricted to a small region in the ventral forebrain.
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| TS15: rhombomere 4 |
Present |
Regionally restricted |
8G left
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Expression outlines a rectangular domain opposite the branchial arch.
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| TS15: rhombomere 2 |
Present |
Regionally restricted |
8G left
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Expression outlines a rectangular domain opposite the branchial arch.
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Specimen
8H:
embryonic day 8.5
(more )
Note: This is a 8-somite embryo. Double labeled: brown - Dll3; blue - Dll1 (assay MGI:5295481).
Specimen
8H:
(close )
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Genetic Background:
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involves: C57BL/6 * DBA
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Age:
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embryonic day 8.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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This is a 8-somite embryo. Double labeled: brown - Dll3; blue - Dll1 (assay MGI:5295481).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS13: trunk unsegmented mesenchyme |
Present |
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8H
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Expressed in a domain at the anterior of the presomitic mesoderm. Posterior to this, Dll1 and Dll3 are coexpressed with different relative levels creating 4 domains.
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Specimen
8I:
embryonic day 8.5
(more )
Note: This is a 10-somite embryo. Double labeled: brown - Dll3; blue - Dll1 (assay MGI:5295481).
Specimen
8I:
(close )
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Genetic Background:
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involves: C57BL/6 * DBA
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Age:
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embryonic day 8.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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This is a 10-somite embryo. Double labeled: brown - Dll3; blue - Dll1 (assay MGI:5295481).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS13: trunk somite |
Present |
Regionally restricted |
8I
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Expression in the anterior boundary of the nascent somite and the somite in the process of of forming.
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| TS13: trunk unsegmented mesenchyme |
Present |
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8I
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Colocalise within the presomitic mesoderm with Dll1 expression.
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Specimen
8J top:
embryonic day 9.0
(more )
Specimen
8J top:
(close )
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Genetic Background:
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involves: C57BL/6 * DBA
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Age:
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embryonic day 9.0
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS14: trunk somite |
Present |
Regionally restricted |
8J top
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Expressed in a broad band at the anterior boundary of the forming somite. This domain narrows to a faint band at the anterior boundary of the nascent somite. Not present in mature somites.
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| TS14: trunk unsegmented mesenchyme |
Present |
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8J top
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Colocalise within the presomitic mesoderm with Dll1 expression.
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Specimen
not shown:
embryonic day 12.5
(more )
Specimen
not shown:
(close )
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Genetic Background:
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involves: C57BL/6 * DBA
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: hindbrain |
Present |
Regionally restricted |
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Only expressed in a subset of the regions of the hindbrain containing Dll1 transcripts.
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Analysis Tools