Gene Expression Data
Assay Details
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Assay
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Reference:
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J:148991
Barkic M, et al., The p53 tumor suppressor causes congenital malformations in Rpl24-deficient mice and promotes their survival. Mol Cell Biol. 2009 May;29(10):2489-504
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|
Assay type:
|
Immunohistochemistry
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|
MGI Accession ID:
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MGI:5295372
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Gene symbol:
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Casp3
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Gene name:
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caspase 3
|
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Antibody:
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Anti-Caspase 3
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Detection system:
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Secondary antibody coupled to horseradish peroxidase
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|
Assay notes:
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The antibody recognized the activated from of the protein.
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Results
|
Specimen
2F wt:
embryonic day 7.5
(more  )
Specimen
2F wt:
(close  )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 7.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
10% Formalin
|
|
Embedding:
|
Paraffin
|
Specimen
2F L24 Bst/+:
embryonic day 7.5; Rpl24Bst/Rpl24 +
(more )
Specimen
2F L24 Bst/+:
(close )
|
Genetic Background:
|
B6.BKS-Rpl24Bst
|
|
Mutant Allele(s):
|
Rpl24Bst/Rpl24+
|
|
Age:
|
embryonic day 7.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
10% Formalin
|
|
Embedding:
|
Paraffin
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS11: embryo |
Trace |
|
2F L24 Bst/+
|
|
Specimen
3G wt & 3H:
embryonic day 10.5
(more )
Note: Fig. 3H shows higher magnification of select regions from 3G.
Specimen
3G wt & 3H:
(close )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 10.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
10% Formalin
|
|
Embedding:
|
Paraffin
|
|
Note:
|
Fig. 3H shows higher magnification of select regions from 3G.
|
Specimen
3G L24 Bst/+ & 3H:
embryonic day 10.5; Rpl24Bst/Rpl24 +
(more )
Note: Fig. 3H shows higher magnification of select regions from 3G.
Specimen
3G L24 Bst/+ & 3H:
(close )
|
Genetic Background:
|
B6.BKS-Rpl24Bst
|
|
Mutant Allele(s):
|
Rpl24Bst/Rpl24+
|
|
Age:
|
embryonic day 10.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
10% Formalin
|
|
Embedding:
|
Paraffin
|
|
Note:
|
Fig. 3H shows higher magnification of select regions from 3G.
|
Specimen
S3A wt:
embryonic day 10.5
(more )
Specimen
S3A wt:
(close )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 10.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
10% Formalin
|
|
Embedding:
|
Paraffin
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS17: optic cup |
Absent |
|
S3A apoptosis wt
|
There was no expression in the retina.
|
Specimen
S3A L24 Bst/+:
embryonic day 10.5; Rpl24Bst/Rpl24 +
(more )
Specimen
S3A L24 Bst/+:
(close )
|
Genetic Background:
|
B6.BKS-Rpl24Bst
|
|
Mutant Allele(s):
|
Rpl24Bst/Rpl24+
|
|
Age:
|
embryonic day 10.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
10% Formalin
|
|
Embedding:
|
Paraffin
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS17: optic cup |
Absent |
|
S3A apoptosis L24 Bst/+
|
There was no expression in the retina.
|
Specimen
S3B wt:
embryonic day 11.5
(more )
Specimen
S3B wt:
(close )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 11.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
10% Formalin
|
|
Embedding:
|
Paraffin
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS19: retina |
Absent |
|
S3B apoptosis wt
|
|
Specimen
S3B L24 Bst/+:
embryonic day 11.5; Rpl24Bst/Rpl24 +
(more )
Specimen
S3B L24 Bst/+:
(close )
|
Genetic Background:
|
B6.BKS-Rpl24Bst
|
|
Mutant Allele(s):
|
Rpl24Bst/Rpl24+
|
|
Age:
|
embryonic day 11.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
10% Formalin
|
|
Embedding:
|
Paraffin
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS19: retina |
Absent |
|
S3B apoptosis L24 Bst/+
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|
Specimen
S4B wt L:
embryonic day 11.5
(more )
Specimen
S4B wt L:
(close )
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Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 11.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
10% Formalin
|
|
Embedding:
|
Paraffin
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS19: liver |
Absent |
|
S4B wt L
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|
Specimen
S4B L24 Bst/+ L:
embryonic day 11.5; Rpl24Bst/Rpl24 +
(more )
Specimen
S4B L24 Bst/+ L:
(close )
|
Genetic Background:
|
B6.BKS-Rpl24Bst
|
|
Mutant Allele(s):
|
Rpl24Bst/Rpl24+
|
|
Age:
|
embryonic day 11.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
10% Formalin
|
|
Embedding:
|
Paraffin
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS19: liver |
Absent |
|
S4B L24 Bst/+ L
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|
Specimen
S4B wt M:
embryonic day 11.5
(more )
Specimen
S4B wt M:
(close )
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Genetic Background:
|
Not Specified
|
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Age:
|
embryonic day 11.5
|
|
Sex:
|
Not Specified
|
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Type:
|
section
|
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Fixation:
|
10% Formalin
|
|
Embedding:
|
Paraffin
|
Specimen
S4B L24 Bst/+ M:
embryonic day 11.5; Rpl24Bst/Rpl24 +
(more )
Specimen
S4B L24 Bst/+ M:
(close )
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Genetic Background:
|
B6.BKS-Rpl24Bst
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|
Mutant Allele(s):
|
Rpl24Bst/Rpl24+
|
|
Age:
|
embryonic day 11.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
10% Formalin
|
|
Embedding:
|
Paraffin
|
Specimen
S4B wt RH:
embryonic day 11.5
(more )
Specimen
S4B wt RH:
(close )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 11.5
|
|
Sex:
|
Not Specified
|
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Type:
|
section
|
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Fixation:
|
10% Formalin
|
|
Embedding:
|
Paraffin
|
Specimen
S4B L24 Bst/+ RH:
embryonic day 11.5; Rpl24Bst/Rpl24 +
(more )
Specimen
S4B L24 Bst/+ RH:
(close )
|
Genetic Background:
|
B6.BKS-Rpl24Bst
|
|
Mutant Allele(s):
|
Rpl24Bst/Rpl24+
|
|
Age:
|
embryonic day 11.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
10% Formalin
|
|
Embedding:
|
Paraffin
|
Specimen
S4B wt NTR:
embryonic day 11.5
(more )
Specimen
S4B wt NTR:
(close )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 11.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
10% Formalin
|
|
Embedding:
|
Paraffin
|
Specimen
S4B L24 Bst/+ NTR:
embryonic day 11.5; Rpl24Bst/Rpl24 +
(more )
Specimen
S4B L24 Bst/+ NTR:
(close )
|
Genetic Background:
|
B6.BKS-Rpl24Bst
|
|
Mutant Allele(s):
|
Rpl24Bst/Rpl24+
|
|
Age:
|
embryonic day 11.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
10% Formalin
|
|
Embedding:
|
Paraffin
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS19: neural tube |
Present |
Single cells |
S4B L24 Bst/+ NTR
|
Only a small number of apoptotic cells were detected in the posterior part of the neural tube.
|
Specimen
S4B wt NTT:
embryonic day 11.5
(more )
Specimen
S4B wt NTT:
(close )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 11.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
10% Formalin
|
|
Embedding:
|
Paraffin
|
Specimen
S4B L24 Bst/+ NTT:
embryonic day 11.5; Rpl24Bst/Rpl24 +
(more )
Specimen
S4B L24 Bst/+ NTT:
(close )
|
Genetic Background:
|
B6.BKS-Rpl24Bst
|
|
Mutant Allele(s):
|
Rpl24Bst/Rpl24+
|
|
Age:
|
embryonic day 11.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
10% Formalin
|
|
Embedding:
|
Paraffin
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS19: tail neural tube |
Present |
Single cells |
S4B L24 Bst/+ NTT
|
Only a small number of apoptotic cells were detected in the posterior part of the neural tube.
|
Specimen
4A wt Thymus:
postnatal week 6
(more )
Specimen
4A wt Thymus:
(close )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
postnatal week 6
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
10% Formalin
|
|
Embedding:
|
Paraffin
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS28: thymus |
Absent |
|
4A apoptosis wt Thymus
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|
Specimen
4A L24 Bst/+ Thymus:
postnatal week 6; Rpl24Bst/Rpl24 +
(more )
Specimen
4A L24 Bst/+ Thymus:
(close )
|
Genetic Background:
|
B6.BKS-Rpl24Bst
|
|
Mutant Allele(s):
|
Rpl24Bst/Rpl24+
|
|
Age:
|
postnatal week 6
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
10% Formalin
|
|
Embedding:
|
Paraffin
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS28: thymus |
Absent |
|
4A apoptosis L24 Bst+/- Thymus
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|
Specimen
4A wt Spleen:
postnatal week 6
(more )
Specimen
4A wt Spleen:
(close )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
postnatal week 6
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
10% Formalin
|
|
Embedding:
|
Paraffin
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS28: spleen |
Absent |
|
4A apoptosis wt Spleen
|
|
Specimen
4A L24 Bst/+ Spleen:
postnatal week 6; Rpl24Bst/Rpl24 +
(more )
Specimen
4A L24 Bst/+ Spleen:
(close )
|
Genetic Background:
|
B6.BKS-Rpl24Bst
|
|
Mutant Allele(s):
|
Rpl24Bst/Rpl24+
|
|
Age:
|
postnatal week 6
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
10% Formalin
|
|
Embedding:
|
Paraffin
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS28: spleen |
Absent |
|
4A apoptosis L24 Bst+/- Spleen
|
|
Specimen
4A wt S. intestine:
postnatal week 6
(more )
Specimen
4A wt S. intestine:
(close )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
postnatal week 6
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
10% Formalin
|
|
Embedding:
|
Paraffin
|
Specimen
4A L24 Bst/+ S. intestine:
postnatal week 6; Rpl24Bst/Rpl24 +
(more )
Specimen
4A L24 Bst/+ S. intestine:
(close )
|
Genetic Background:
|
B6.BKS-Rpl24Bst
|
|
Mutant Allele(s):
|
Rpl24Bst/Rpl24+
|
|
Age:
|
postnatal week 6
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
10% Formalin
|
|
Embedding:
|
Paraffin
|
Specimen
4A wt Kidney:
postnatal week 6
(more )
Specimen
4A wt Kidney:
(close )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
postnatal week 6
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
10% Formalin
|
|
Embedding:
|
Paraffin
|
Specimen
4A L24 Bst/+ Kidney:
postnatal week 6; Rpl24Bst/Rpl24 +
(more )
Specimen
4A L24 Bst/+ Kidney:
(close )
|
Genetic Background:
|
B6.BKS-Rpl24Bst
|
|
Mutant Allele(s):
|
Rpl24Bst/Rpl24+
|
|
Age:
|
postnatal week 6
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
10% Formalin
|
|
Embedding:
|
Paraffin
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS28: metanephros |
Absent |
|
4A apoptosis L24 Bst+/- Kidney
|
|
Specimen
4A wt Liver:
postnatal week 6
(more )
Specimen
4A wt Liver:
(close )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
postnatal week 6
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
10% Formalin
|
|
Embedding:
|
Paraffin
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS28: liver |
Absent |
|
4A apoptosis wt Liver
|
|
Specimen
4A L24 Bst/+ Liver:
postnatal week 6; Rpl24Bst/Rpl24 +
(more )
Specimen
4A L24 Bst/+ Liver:
(close )
|
Genetic Background:
|
B6.BKS-Rpl24Bst
|
|
Mutant Allele(s):
|
Rpl24Bst/Rpl24+
|
|
Age:
|
postnatal week 6
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
10% Formalin
|
|
Embedding:
|
Paraffin
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS28: liver |
Absent |
|
4A apoptosis L24 Bst+/- Liver
|
|
Specimen
6B L24 Bst/+ RH:
embryonic day 10.5; Rpl24Bst/Rpl24 +
(more )
Specimen
6B L24 Bst/+ RH:
(close )
|
Genetic Background:
|
B6.BKS-Rpl24Bst
|
|
Mutant Allele(s):
|
Rpl24Bst/Rpl24+
|
|
Age:
|
embryonic day 10.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
10% Formalin
|
|
Embedding:
|
Paraffin
|
Specimen
6B L24 Bst/+ NTR:
embryonic day 10.5; Rpl24Bst/Rpl24 +
(more )
Specimen
6B L24 Bst/+ NTR:
(close )
|
Genetic Background:
|
B6.BKS-Rpl24Bst
|
|
Mutant Allele(s):
|
Rpl24Bst/Rpl24+
|
|
Age:
|
embryonic day 10.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
10% Formalin
|
|
Embedding:
|
Paraffin
|
Specimen
6B L24 Bst/+ NTT:
embryonic day 10.5; Rpl24Bst/Rpl24 +
(more )
Specimen
6B L24 Bst/+ NTT:
(close )
|
Genetic Background:
|
B6.BKS-Rpl24Bst
|
|
Mutant Allele(s):
|
Rpl24Bst/Rpl24+
|
|
Age:
|
embryonic day 10.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
10% Formalin
|
|
Embedding:
|
Paraffin
|
Specimen
6B L24 Bst/+;p53-/- RH:
(close )
|
Genetic Background:
|
B6.Cg-Trp53tm1Tyj Rpl24Bst
|
|
Mutant Allele(s):
|
Rpl24Bst/Rpl24+
Trp53tm1Tyj/Trp53tm1Tyj
|
|
Age:
|
embryonic day 10.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
10% Formalin
|
|
Embedding:
|
Paraffin
|
Specimen
6B L24 Bst/+;p53-/- NTR:
(close )
|
Genetic Background:
|
B6.Cg-Trp53tm1Tyj Rpl24Bst
|
|
Mutant Allele(s):
|
Rpl24Bst/Rpl24+
Trp53tm1Tyj/Trp53tm1Tyj
|
|
Age:
|
embryonic day 10.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
10% Formalin
|
|
Embedding:
|
Paraffin
|
Specimen
6B L24 Bst/+;p53-/- NTT:
(close )
|
Genetic Background:
|
B6.Cg-Trp53tm1Tyj Rpl24Bst
|
|
Mutant Allele(s):
|
Rpl24Bst/Rpl24+
Trp53tm1Tyj/Trp53tm1Tyj
|
|
Age:
|
embryonic day 10.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
10% Formalin
|
|
Embedding:
|
Paraffin
|
Specimen
6B L24 Bst/+;p53+/- RH:
embryonic day 10.5; Rpl24Bst/Rpl24 +, Trp53tm1Tyj/Trp53 +
(more )
Specimen
6B L24 Bst/+;p53+/- RH:
(close )
|
Genetic Background:
|
B6.Cg-Trp53tm1Tyj Rpl24Bst
|
|
Mutant Allele(s):
|
Rpl24Bst/Rpl24+
Trp53tm1Tyj/Trp53+
|
|
Age:
|
embryonic day 10.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
10% Formalin
|
|
Embedding:
|
Paraffin
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS17: hindbrain roof plate |
Trace |
Single cells |
6B L24 Bst+/-;p53+/- RH
|
The presence of one p53 allele was insufficient to trigger a significant apoptotic response in neural tissues.
|
Specimen
6B L24 Bst/+;p53+/- NTR:
embryonic day 10.5; Rpl24Bst/Rpl24 +, Trp53tm1Tyj/Trp53 +
(more )
Specimen
6B L24 Bst/+;p53+/- NTR:
(close )
|
Genetic Background:
|
B6.Cg-Trp53tm1Tyj Rpl24Bst
|
|
Mutant Allele(s):
|
Rpl24Bst/Rpl24+
Trp53tm1Tyj/Trp53+
|
|
Age:
|
embryonic day 10.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
10% Formalin
|
|
Embedding:
|
Paraffin
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS17: neural tube |
Trace |
Single cells |
6B L24 Bst+/-;p53+/- NTR
|
The presence of one p53 allele was insufficient to trigger a significant apoptotic response in neural tissues.
|
Specimen
6B L24 Bst/+;p53+/- NTT:
embryonic day 10.5; Rpl24Bst/Rpl24 +, Trp53tm1Tyj/Trp53 +
(more )
Specimen
6B L24 Bst/+;p53+/- NTT:
(close )
|
Genetic Background:
|
B6.Cg-Trp53tm1Tyj Rpl24Bst
|
|
Mutant Allele(s):
|
Rpl24Bst/Rpl24+
Trp53tm1Tyj/Trp53+
|
|
Age:
|
embryonic day 10.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
10% Formalin
|
|
Embedding:
|
Paraffin
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS17: tail neural tube |
Trace |
Single cells |
6B L24 Bst+/-;p53+/- NTT
|
The presence of one p53 allele was insufficient to trigger a significant apoptotic response in neural tissues.
|
|
Analysis Tools