Gene Expression Data
Assay Details
Assay
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Reference:
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J:162481
Hoesel B, et al., Combination of in silico and insitu hybridisation approaches to identify potential Dll1 associated miRNAs during mouse embryogenesis. Gene Expr Patterns. 2010 Sep;10(6):265-73
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:5287637
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Gene symbol:
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Mir1a-2
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Gene name:
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microRNA 1a-2
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Probe:
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miR-1 probe
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Probe preparation:
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Antisense labelled with digoxigenin DNA
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Visualized with:
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Alkaline phosphatase
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Assay notes:
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The mature microRNA product is encoded by multiple genes. The double-DIG labeled probe has label at both the 3' and 5' ends.
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Results
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Specimen
2A-left:
embryonic day 9.5
(more )
Note: A single-DIG labeled (SL) probe was used on this specimen.
Specimen
2A-left:
(close )
Genetic Background:
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C3HeB/FeJ
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Age:
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embryonic day 9.5
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Age Note:
|
Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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A single-DIG labeled (SL) probe was used on this specimen.
|
Specimen
2A-right:
embryonic day 9.5
(more )
Note: A single-DIG labeled (SL) probe was used on this specimen.
Specimen
2A-right:
(close )
Genetic Background:
|
C3HeB/FeJ
|
Age:
|
embryonic day 9.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
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Not Specified
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Type:
|
whole mount
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Fixation:
|
4% Paraformaldehyde
|
Note:
|
A single-DIG labeled (SL) probe was used on this specimen.
|
Structure |
Level |
Pattern |
Image |
Note |
TS15: myotome |
Present |
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2A-right
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The staining seen in the neural tube is non-specific.
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Specimen
2B-left:
embryonic day 9.5
(more )
Note: A double-DIG labeled (DL) probe was used on this specimen.
Specimen
2B-left:
(close )
Genetic Background:
|
C3HeB/FeJ
|
Age:
|
embryonic day 9.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
A double-DIG labeled (DL) probe was used on this specimen.
|
Specimen
2B-right:
embryonic day 9.5
(more )
Note: A double-DIG labeled (DL) probe was used on this specimen.
Specimen
2B-right:
(close )
Genetic Background:
|
C3HeB/FeJ
|
Age:
|
embryonic day 9.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
A double-DIG labeled (DL) probe was used on this specimen.
|
Structure |
Level |
Pattern |
Image |
Note |
TS15: myotome |
Present |
|
2B-right
|
The staining seen in the neural tube is non-specific.
|
Specimen
3A:
embryonic day 10.5
(more )
Note: A single-DIG labeled (SL) probe was used on this specimen.
Specimen
3A:
(close )
Genetic Background:
|
C3HeB/FeJ
|
Age:
|
embryonic day 10.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
whole mount
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Fixation:
|
4% Paraformaldehyde
|
Note:
|
A single-DIG labeled (SL) probe was used on this specimen.
|
Specimen
3B:
embryonic day 10.5
(more )
Note: A double-DIG labeled (DL) probe was used on this specimen.
Specimen
3B:
(close )
Genetic Background:
|
C3HeB/FeJ
|
Age:
|
embryonic day 10.5
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Age Note:
|
Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
|
4% Paraformaldehyde
|
Note:
|
A double-DIG labeled (DL) probe was used on this specimen.
|
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