Probe:	miR-130b probe
Probe preparation:	Antisense labelled with digoxigenin DNA
Visualized with:	Alkaline phosphatase
Assay notes:	The double-DIG labeled probe has label at both the 3' and 5' ends.

Structure	Level	Pattern	Image	Note
TS15: somite	Present	Widespread	2P	Expression is detected in all somites along the posterior anterior axis.

Structure	Level	Pattern	Image	Note
TS15: embryo	Weak	Widespread	2R
TS15: somite	Present	Widespread	2R	Expression is detected in all somites along the posterior anterior axis.
TS15: heart	Absent		2R

Structure	Level	Pattern	Image	Note
TS17: forelimb bud	Present		3K
TS17: hindlimb bud	Present		3K
TS17: neural tube	Present		3K
TS17: somite	Present		3K

Structure	Level	Pattern	Image	Note
TS17: forelimb bud	Present		3L-first
TS17: hindlimb bud	Present		3L-first
TS17: neural tube	Present		3L-first
TS17: somite	Present		3L-first

Structure	Level	Pattern	Image	Note
TS17: limb	Present		3L-second
TS17: neural tube	Present		3L-second
TS17: somite	Present		3L-second

Structure	Level	Pattern	Image	Note
TS17: neural tube	Present		3L-third

Structure	Level	Pattern	Image	Note
TS17: somite	Present	Non-Uniform	3L-fourth	In older, more anterior, somites, expression is strongest in the posterior compartment of each somite.

Structure	Level	Pattern	Image	Note
TS17: somite	Present	Non-Uniform	3L-fifth	In the more recently formed, most posterior somites, expression is present in the whole somite; it is detected at the inner epithelial walls without restriction to the posterior compartments.

Structure	Level	Pattern	Image	Note
TS15: facio-acoustic preganglion complex	Present		4C
TS15: trigeminal preganglion	Present		4C
TS15: 1st branchial arch	Present		4C
TS15: 2nd branchial arch	Present		4C
TS15: otocyst	Present		4C

Structure	Level	Pattern	Image	Note
TS15: future brain	Present
TS15: head mesenchyme	Present
TS15: neural tube	Present

Structure	Level	Pattern	Image	Note
TS17: trigeminal ganglion	Present		4H
TS17: acoustic ganglion	Present		4H	Expression is present in the VII/VIII cranial ganglia.
TS17: facial ganglion	Present		4H	Expression is present in the VII/VIII cranial ganglia.
TS17: 1st branchial arch	Present		4H
TS17: 2nd branchial arch	Present		4H
TS17: otocyst	Present		4H

Structure	Level	Pattern	Image	Note
TS17: forebrain	Present
TS17: midbrain	Present

Structure	Level	Pattern	Image	Note
TS17: neural tube	Present		5F
TS17: trunk somite	Present	Regionally restricted	5F	Expression is close to the epithelial border of a somite.

Structure	Level	Pattern	Image	Note
TS17: trunk somite	Present	Regionally restricted	5G	Expression is present in the second and third most posterior somite.