Probe:	miR-103 probe
Probe preparation:	Antisense labelled with digoxigenin DNA
Visualized with:	Alkaline phosphatase
Assay notes:	The mature microRNA product is encoded by multiple genes. The double-DIG labeled probe has label at both the 3' and 5' ends.

Structure	Level	Pattern	Image	Note
TS15: somite	Present	Widespread	2H	Expression is detected in all somites along the posterior anterior axis.

Structure	Level	Pattern	Image	Note
TS15: embryo	Weak	Widespread	2I
TS15: somite	Present	Widespread	2I	Expression is detected in all somites along the posterior anterior axis.
TS15: heart	Absent		2I

Structure	Level	Pattern	Image	Note
TS17: forelimb bud	Present		3F-first
TS17: hindlimb bud	Present		3F-first
TS17: neural tube	Present		3F-first
TS17: somite	Present		3F-first

Structure	Level	Pattern	Image	Note
TS17: limb	Present		3F-second
TS17: neural tube	Present		3F-second
TS17: somite	Present		3F-second

Structure	Level	Pattern	Image	Note
TS17: dermomyotome	Present		3F-third
TS17: limb	Present		3F-third
TS17: neural tube	Present		3F-third

Structure	Level	Pattern	Image	Note
TS17: neural tube	Present		3F-fourth
TS17: somite	Present	Non-Uniform	3F-fourth	In older, more anterior, somites, expression is strongest in the posterior compartment of each somite.

Structure	Level	Pattern	Image	Note
TS17: somite	Present	Regionally restricted	3F-fifth	In the five most posterior somites, expression is present in the whole somite; it is detected at the inner epithelial walls without restriction to the posterior compartments.

Structure	Level	Pattern	Image	Note
TS17: forelimb bud	Present		3G
TS17: hindlimb bud	Present		3G
TS17: neural tube	Present		3G
TS17: somite	Present		3G

Structure	Level	Pattern	Image	Note
TS15: facio-acoustic preganglion complex	Present		4A
TS15: trigeminal preganglion	Present		4A
TS15: 1st branchial arch	Present		4A
TS15: 2nd branchial arch	Present		4A
TS15: otocyst	Present		4A

Structure	Level	Pattern	Image	Note
TS15: future brain	Present
TS15: head mesenchyme	Present
TS15: neural tube	Present

Structure	Level	Pattern	Image	Note
TS17: trigeminal ganglion	Present		4F
TS17: acoustic ganglion	Present		4F	Expression is present in the VII/VIII cranial ganglia.
TS17: facial ganglion	Present		4F	Expression is present in the VII/VIII cranial ganglia.
TS17: 1st branchial arch	Present		4F
TS17: 2nd branchial arch	Present		4F
TS17: otocyst	Present		4F

Structure	Level	Pattern	Image	Note
TS17: forebrain	Present
TS17: midbrain	Present

Structure	Level	Pattern	Image	Note
TS17: neural tube	Present	Non-Uniform	5A	The strongest staining is found in the neuroepithelium towards the lumen of the tube.
TS17: trunk somite	Present	Regionally restricted	5A	Expression is present in the epithelia of somites towards the somitocoele.

Structure	Level	Pattern	Image	Note
TS17: trunk somite	Strong	Non-Uniform	5B	Expression is present throughout the anterior and posterior compartments within the somitic epithelia, with strongest expression towards the lumen of the somite.