Probe:	Prickle1 cDNA2
Probe preparation:	Antisense labelled with P33 RNA
Visualized with:	Autoradiography

Structure	Level	Pattern	Image	Note
TS17: nervous system	Moderate		1H	Neural expression was relatively higher in postmitotic differentiation fields than in ventricular zones.
TS17: limb	Present		1H
TS17: embryo	Present	Non-Uniform	1H	Expression was detected at moderate intensity in neural tissue, and to a lesser level in non-neural tissue, particularly in limb buds.

Structure	Level	Pattern	Image	Note
TS20: cartilage	Strong		2U
TS20: ganglion	Moderate		2U
TS20: skin	Moderate		2U

Structure	Level	Pattern	Image	Note
TS20: brain	Present	Regionally restricted	2V , 2W	Expression was restricted to periventricular and paramedian nuclei of the brainstem.

Structure	Level	Pattern	Image	Note
TS22: cartilage	Strong
TS22: brain	Weak	Regionally restricted		The brain was mostly unlabelled, with the exception of the immature facial nerve nucleus in the brainstem and the cortical plate.
TS22: brainstem	Weak	Regionally restricted		The brain was mostly unlabelled, with the exception of the immature facial nerve nucleus in the brainstem and the cortical plate.
TS22: cortical plate	Weak

Structure	Level	Pattern	Image	Note
TS25: brainstem	Present	Regionally restricted		Expression was present in the reticular formation but was very low or undetectable in the cochleovestibular nuclei.
TS25: cerebellum	Ambiguous	Regionally restricted		Expression was very low or undetectable in the external granule layer.
TS25: diencephalon ventricular layer	Ambiguous			Expression was very low or undetectable.
TS25: hypothalamus	Ambiguous			Expression was very low or undetectable.
TS25: midbrain tegmentum	Ambiguous			Expression was very low or undetectable.
TS25: midbrain ventricular layer	Ambiguous			Expression was very low or undetectable.
TS25: tectum	Ambiguous			Expression was very low or undetectable.
TS25: thalamus	Ambiguous	Regionally restricted		Expression was very low or undetectable in the dorsal and ventral thalamus.
TS25: retina	Ambiguous	Regionally restricted		Expression was very low or undetectable in the ventricular zone and ganglion cells.
TS25: cortical plate	Present	Regionally restricted		Expression was detected in the cortical plate proper but was very low or undetectable in the cortical plate ventricular zone/subventricular zone.
TS25: corpus striatum	Ambiguous	Regionally restricted		Expression was very low or undetectable in the striatum ventricular zone/subventricular zone and in the striatum.
TS25: main olfactory bulb	Ambiguous	Regionally restricted		Expression was very low or undetectable in the olfactory bulb and in mitral cells.

Structure	Level	Pattern	Image	Note
TS25: cartilage	Present		4G1
TS25: lens	Present		4G1
TS25: medulla oblongata	Strong	Regionally restricted	4G1	A very high signal was associated specifically with the inferior olivary complex.

Structure	Level	Pattern	Image	Note
TS25: cortical plate	Moderate	Regionally restricted	4G2	Expression was detected in the medial and dorsal sectors of the cortical plate.

Structure	Level	Pattern	Image	Note
TS27: brain	Weak		5K
TS27: cerebral cortex	Moderate	Regionally restricted	5K	Expression was moderate in the cortical plate.
TS27: inferior olivary nucleus	Very strong		5L , 5K

Structure	Level	Pattern	Image	Note
TS28: cerebellum external granule cell layer	Ambiguous			Expression was very low or undetectable.
TS28: diencephalon	Ambiguous	Regionally restricted		Expression was very low or undetectable in the diencephalon ventricular zone.
TS28: dentate gyrus	Present
TS28: dorsal thalamus	Present
TS28: hippocampus pyramidal cell layer	Present
TS28: main olfactory bulb mitral cell layer	Present
TS28: hypothalamus	Ambiguous			Expression was very low or undetectable.
TS28: prethalamus	Ambiguous			Expression was very low or undetectable.
TS28: cerebral cortex	Present	Regionally restricted		Expression was detected in the cortical plate proper but was very low or undetectable in the cortical plate ventricular zone/subventricular zone.
TS28: corpus striatum	Ambiguous			Expression was very low or undetectable in the striatum and the ventricular zone/subventricular zone.
TS28: rostral migratory stream	Ambiguous			Expression was very low or undetectable in the rostral migratory stream.
TS28: main olfactory bulb granule cell layer	Strong

Structure	Level	Pattern	Image	Note
TS28: neocortex	Present	Regionally restricted	6S	Expression was detected in the inner aspect of the cortical plate but not in periventricular layers.

Structure	Level	Pattern	Image	Note
TS28: hippocampus	Present		6T

Structure	Level	Pattern	Image	Note
TS28: main olfactory bulb granule cell layer	Strong		6U
TS28: main olfactory bulb	Present	Regionally restricted	6U	Expression was detected in the granular layer of the olfactory bulb but the rostral migratory stream was negative.
TS28: neocortex	Present	Regionally restricted	6U	Expression was detected in the inner aspect of the cortical plate in the neocortex but was negative in the rostral migratory stream.
TS28: rostral migratory stream	Absent		6U

Structure	Level	Pattern	Image	Note
TS28: inferior olivary nucleus	Present

Structure	Level	Pattern	Image	Note
TS28: telencephalon	Present		7G
TS28: cerebral cortex	Moderate	Diffuse	7G
TS28: dentate gyrus	Moderate	Diffuse	7G
TS28: hippocampus pyramidal cell layer	Moderate	Diffuse	7G
TS28: main olfactory bulb granule cell layer	Moderate	Diffuse	7G