Gene Expression Data
Assay Details
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Assay
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Reference:
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J:90402
Merrill BJ, et al., Tcf3: a transcriptional regulator of axis induction in the early embryo. Development. 2004 Jan;131(2):263-74
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:4943174
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Gene symbol:
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T
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Gene name:
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brachyury, T-box transcription factor T
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Probe:
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brachyury probe5
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Results
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Specimen
5C:
embryonic day 8.5
(more  )
Note: Data for +/+ or +/- embryos were indistinguishable and are referred to as 'wild-type'; thus the pictured specimen could have been a heterozygous animal.
Specimen
5C:
(close  )
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Genetic Background:
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involves: 129 * C57BL/6
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Age:
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embryonic day 8.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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Data for +/+ or +/- embryos were indistinguishable and are referred to as 'wild-type'; thus the pictured specimen could have been a heterozygous animal.
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Specimen
5C':
embryonic day 8.5
(more )
Note: Data for +/+ or +/- embryos were indistinguishable and are referred to as 'wild-type'; thus the pictured specimen could have been a heterozygous animal.
Specimen
5C':
(close )
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Genetic Background:
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involves: 129 * C57BL/6
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Age:
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embryonic day 8.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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Data for +/+ or +/- embryos were indistinguishable and are referred to as 'wild-type'; thus the pictured specimen could have been a heterozygous animal.
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Specimen
Heterozygote:
embryonic day 8.5; Tcf7l1tm2Efu/Tcf7l1 +
(more )
Specimen
Heterozygote:
(close )
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Genetic Background:
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involves: 129 * C57BL/6
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Mutant Allele(s):
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Tcf7l1tm2Efu/Tcf7l1+
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Age:
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embryonic day 8.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
5D left:
embryonic day 8.5; Tcf7l1tm2Efu/ Tcf7l1tm2Efu
(more )
Note: The aberrant patterns seen in mutants vary.
Specimen
5D left:
(close )
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Genetic Background:
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involves: 129/Sv * C57BL/6
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Mutant Allele(s):
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Tcf7l1tm2Efu/Tcf7l1tm2Efu
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Age:
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embryonic day 8.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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The aberrant patterns seen in mutants vary.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS13: node |
Present |
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5D left
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| TS13: notochord |
Present |
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5D left
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| TS13: embryo |
Present |
Regionally restricted |
5D left
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Expression is often grossly expanded in the mutant, as a direct reflection of the degree of expanded/duplicated node and notochordal cells in a given embryo.
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Specimen
5D center:
embryonic day 8.5; Tcf7l1tm2Efu/ Tcf7l1tm2Efu
(more )
Note: The aberrant patterns seen in mutants vary.
Specimen
5D center:
(close )
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Genetic Background:
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involves: 129/Sv * C57BL/6
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Mutant Allele(s):
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Tcf7l1tm2Efu/Tcf7l1tm2Efu
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Age:
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embryonic day 8.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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The aberrant patterns seen in mutants vary.
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Specimen
5D right:
embryonic day 8.5; Tcf7l1tm2Efu/ Tcf7l1tm2Efu
(more )
Note: The aberrant patterns seen in mutants vary.
Specimen
5D right:
(close )
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Genetic Background:
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involves: 129/Sv * C57BL/6
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Mutant Allele(s):
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Tcf7l1tm2Efu/Tcf7l1tm2Efu
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Age:
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embryonic day 8.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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The aberrant patterns seen in mutants vary.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS13: node |
Present |
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5D right
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| TS13: notochord |
Present |
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5D right
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| TS13: embryo |
Present |
Regionally restricted |
5D right
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Expression is often grossly expanded in the mutant, as a direct reflection of the degree of expanded/duplicated node and notochordal cells in a given embryo.
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Specimen
5D':
(close )
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Genetic Background:
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involves: 129/Sv * C57BL/6
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Mutant Allele(s):
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Tcf7l1tm2Efu/Tcf7l1tm2Efu
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Age:
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embryonic day 8.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS13: node |
Present |
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5D'
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| TS13: notochord |
Present |
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5D'
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| TS13: embryo |
Present |
Regionally restricted |
5D'
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Expression is often grossly expanded in the mutant, as a direct reflection of the degree of expanded/duplicated node and notochordal cells in a given embryo.
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Specimen
5G:
embryonic day 8.5; Tcf7l1tm2Efu/ Tcf7l1tm2Efu
(more )
Note: This mutant appears to have axis duplications on opposite sides of the embryo.
Specimen
5G:
(close )
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Genetic Background:
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involves: 129/Sv * C57BL/6
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Mutant Allele(s):
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Tcf7l1tm2Efu/Tcf7l1tm2Efu
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Age:
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embryonic day 8.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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This mutant appears to have axis duplications on opposite sides of the embryo.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS13: primitive streak |
Present |
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5G
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This embryo appears to have a primary and secondary primitive streak.
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| TS13: node |
Present |
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5G
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Expression is often grossly expanded in the mutant, as a direct reflection of the degree of expanded/duplicated node and notochordal cells in a given embryo.
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| TS13: notochord |
Present |
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5G
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Expression is often grossly expanded in the mutant, as a direct reflection of the degree of expanded/duplicated node and notochordal cells in a given embryo.
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Specimen
5G':
embryonic day 8.5; Tcf7l1tm2Efu/ Tcf7l1tm2Efu
(more )
Note: This mutant appears to have axis duplications on opposite sides of the embryo.
Specimen
5G':
(close )
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Genetic Background:
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involves: 129/Sv * C57BL/6
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Mutant Allele(s):
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Tcf7l1tm2Efu/Tcf7l1tm2Efu
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Age:
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embryonic day 8.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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This mutant appears to have axis duplications on opposite sides of the embryo.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS13: primitive streak |
Present |
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5G'
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This embryo appears to have a primary and secondary primitive streak.
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| TS13: node |
Present |
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5G'
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Expression is often grossly expanded in the mutant, as a direct reflection of the degree of expanded/duplicated node and notochordal cells in a given embryo.
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| TS13: notochord |
Present |
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5G'
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Expression is often grossly expanded in the mutant, as a direct reflection of the degree of expanded/duplicated node and notochordal cells in a given embryo.
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Specimen
6E:
embryonic day 7.0
(more )
Note: Data for +/+ or +/- embryos were indistinguishable and are referred to as 'wild-type'; thus the pictured specimen could have been a heterozygous animal.
Specimen
6E:
(close )
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Genetic Background:
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involves: 129 * C57BL/6
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Age:
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embryonic day 7.0
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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Data for +/+ or +/- embryos were indistinguishable and are referred to as 'wild-type'; thus the pictured specimen could have been a heterozygous animal.
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Specimen
Heterozygote:
embryonic day 7.0; Tcf7l1tm2Efu/Tcf7l1 +
(more )
Specimen
Heterozygote:
(close )
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Genetic Background:
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involves: 129 * C57BL/6
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Mutant Allele(s):
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Tcf7l1tm2Efu/Tcf7l1+
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Age:
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embryonic day 7.0
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
6F:
(close )
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Genetic Background:
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involves: 129/Sv * C57BL/6
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Mutant Allele(s):
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Tcf7l1tm2Efu/Tcf7l1tm2Efu
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Age:
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embryonic day 7.0
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS10: mesendoderm |
Present |
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6F
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Expression is maintained in the mutant. In 57% of cases it adopts a bent or twisted pattern, consistent with the presence of multiple primitive streaks. In 28% of mutants staining intensity is slightly diminished.
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| TS10: primitive streak |
Present |
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6F
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Expression is maintained in the mutant. In 57% of cases it adopts a bent or twisted pattern, consistent with the presence of multiple primitive streaks. In 28% of mutants staining intensity is slightly diminished.
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Specimen
6F':
(close )
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Genetic Background:
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involves: 129/Sv * C57BL/6
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Mutant Allele(s):
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Tcf7l1tm2Efu/Tcf7l1tm2Efu
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Age:
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embryonic day 7.0
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS10: mesendoderm |
Present |
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6F'
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Expression is maintained in the mutant. In 57% of cases it adopts a bent or twisted pattern, consistent with the presence of multiple primitive streaks. In 28% of mutants staining intensity is slightly diminished.
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| TS10: primitive streak |
Present |
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6F'
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Expression is maintained in the mutant. In 57% of cases it adopts a bent or twisted pattern, consistent with the presence of multiple primitive streaks. In 28% of mutants staining intensity is slightly diminished.
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Analysis Tools