Probe:	Gpx4 cDNA4
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase
Assay notes:	The authors did not specify which probe was used, either a full length (Gpx4 cDNA3) or this 3' end probe was used.

Structure	Level	Pattern	Image	Note
TS11: embryo	Strong	Ubiquitous	1A
TS11: embryo ectoderm	Strong	Ubiquitous	1A
TS11: embryo endoderm	Strong	Ubiquitous	1A
TS11: embryo mesoderm	Strong	Ubiquitous	1A
TS11: extraembryonic component	Strong	Ubiquitous	1A
TS11: Reichert's membrane	Absent		1A

Structure	Level	Pattern	Image	Note
TS13: branchial arch	Strong		1C
TS13: embryo mesenchyme	Strong		1C
TS13: future brain neural fold	Strong		1C
TS13: neural ectoderm	Strong		1C
TS13: somite	Strong		1C
TS13: embryo	Present	Regionally restricted	1C	Expression is in the tailbud.
TS13: 1st branchial arch	Present	Regionally restricted	1C	Expression is in the maxillary and mandibular components.
TS13: optic pit	Present		1C
TS13: otic placode	Present		1C

Structure	Level	Pattern	Image	Note
TS15: branchial arch	Present		1E
TS15: forelimb bud	Present		1E
TS15: future forebrain	Present		1E
TS15: heart	Present		1E
TS15: nervous system	Present		1E
TS15: somite	Present		1E
TS15: 1st branchial arch mandibular component	Present		1E
TS15: 1st branchial arch maxillary component	Present		1E
TS15: optic vesicle	Present		1E
TS15: otocyst	Present		1E

Structure	Level	Pattern	Image	Note
TS17: branchial arch	Strong		1G
TS17: eye	Strong		1G
TS17: forebrain	Strong		1G
TS17: forelimb bud	Strong		1G
TS17: fronto-nasal process	Strong		1G
TS17: heart	Strong		1G
TS17: hindlimb bud	Strong		1G
TS17: midbrain	Strong		1G
TS17: somite	Strong		1G
TS17: gut	Weak		1G

Structure	Level	Pattern	Image	Note
TS19: branchial arch	Strong		1I
TS19: eye	Strong		1I
TS19: fronto-nasal process	Strong		1I
TS19: heart	Strong		1I
TS19: liver	Strong		1I
TS19: nasal pit	Strong		1I
TS19: nervous system	Strong		1I
TS19: tail somite	Strong		1I
TS19: trunk somite	Strong		1I
TS19: limb	Strong	Regionally restricted	1I	Expression is at the distal ends of the limb buds.
TS19: gut	Weak		1I

Structure	Level	Pattern	Image	Note
TS21: choroid plexus	Strong		1Q
TS21: foot	Strong		1Q
TS21: gonad	Strong		1Q
TS21: hand	Strong		1Q
TS21: heart	Strong		1Q
TS21: liver	Strong		1Q
TS21: lung	Strong		1Q
TS21: metanephros	Strong		1Q
TS21: nervous system	Strong		1Q
TS21: vertebral cartilage condensation	Strong		1Q
TS21: vibrissa	Strong		1Q
TS21: tail	Strong	Regionally restricted	1Q	Expression is in the distal part.