Probe:	Btg1 cDNA
Probe preparation:	Antisense labelled with digoxigenin RNA

Structure	Level	Pattern	Image	Note
TS15: forelimb bud presumptive apical ectodermal ridge	Strong
TS15: trunk somite	Strong
TS15: tail	Strong
TS15: eye	Moderate
TS15: genitourinary system	Moderate	Regionally restricted		Staining is present in the external genitalia.
TS15: neural tube	Strong			There is strong staining in the medial and lateral neural tube.

Structure	Level	Pattern	Image	Note
TS17: apical ectodermal ridge	Strong
TS17: limb mesenchyme	Moderate	Regionally restricted		Staining is present in the middle limb.
TS17: limb	Moderate	Regionally restricted		Staining is present in the posterior limb.
TS17: trunk somite	Strong
TS17: tail	Strong
TS17: 1st branchial arch maxillary component	Moderate			Staining is present in the maxillary process.
TS17: 1st branchial arch	Moderate			Staining is present in the mandibular arch.
TS17: 2nd branchial arch	Moderate			Staining is present in the hyoid arch.
TS17: eye	Moderate
TS17: genitourinary system	Moderate	Regionally restricted		Staining is present in the external genitalia.
TS17: nasal pit	Moderate			Staining is present in the olfactory placode.
TS17: otocyst	Moderate			Staining is present in the otic pit.
TS17: neural tube	Strong			There is strong staining in the medial and lateral neural tube.

Structure	Level	Pattern	Image	Note
TS19: limb mesenchyme	Strong	Regionally restricted		Staining is present in the progress zone and middle limb.
TS19: limb	Strong	Non-Uniform		Staining is strong in the posterior-distal and posterior proximal regions and moderate in the antero-distal and antero-proximal regions of the limb.
TS19: trunk somite	Strong
TS19: tail	Strong
TS19: maxillary process	Strong
TS19: 1st branchial arch	Strong			Staining is present in the mandibular arch.
TS19: 2nd branchial arch	Strong			Staining is present in the hyoid arch.
TS19: eye	Strong