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Gene Expression Data
Assay Details
Assay
Reference: J:155982 Henry SP, et al., Generation of aggrecan-CreERT2 knockin mice for inducible Cre activity in adult cartilage. Genesis. 2009 Dec;47(12):805-14
Assay type: Recombinase reporter
MGI Accession ID: MGI:4843277
Gene symbol: Acan
Gene name: aggrecan
Reporter: lacZ
Direct Detection
Results
Specimen Fig. 2a E12.5: embryonic day 12.5; Acantm1(cre/ERT2)Crm/Acan+, Gt(ROSA)26Sortm1Sor/Gt(ROSA)26Sor+ (more )
Note: Conditional mutant. Pregnant animals were injected i.p. with 3 mg of 4OH-tamoxifen on E11.5. Embryos were harvested 24 hours later.
Structure Level Pattern Image Note
TS20: embryo Absent 2a E12.5 No staining is observed with 4OHT treatment at E11.5.
Specimen Fig. 2a E13.0: embryonic day 13.0; Acantm1(cre/ERT2)Crm/Acan+, Gt(ROSA)26Sortm1Sor/Gt(ROSA)26Sor+ (more )
Note: Conditional mutant. Pregnant animals were injected i.p. with 3 mg of 4OH-tamoxifen on E12. Embryos were harvested 24 hours later.
Structure Level Pattern Image Note
TS21: forelimb Present 2a E13.0 Staining is first observed in the forelimb with 4OHT treatment on E12.
Specimen Fig. 2a E13.5: embryonic day 13.5; Acantm1(cre/ERT2)Crm/Acan+, Gt(ROSA)26Sortm1Sor/Gt(ROSA)26Sor+ (more )
Note: Conditional mutant. Pregnant animals were injected i.p. with 3 mg of 4OH-tamoxifen on E12.5. Embryos were harvested 24 hours later.
Structure Level Pattern Image Note
TS21: cervical vertebral cartilage condensation Present 2a E13.5 Staining is observed in cartilage of axial skeleton with 4OHT treatment at E12.5.
TS21: lumbar vertebral cartilage condensation Present 2a E13.5 Staining is observed in cartilage of axial skeleton with 4OHT treatment at E12.5.
TS21: sacral vertebral cartilage condensation Present 2a E13.5 Staining is observed in cartilage of axial skeleton with 4OHT treatment at E12.5.
TS21: thoracic vertebral cartilage condensation Present 2a E13.5 Staining is observed in cartilage of axial skeleton with 4OHT treatment at E12.5.
TS21: limb cartilage condensation Present 2a E13.5 Staining is observed in cartilage of appendicular skeleton with 4OHT treatment at E12.5.
Specimen Fig. 2b right: embryonic day 15.5; Acantm1(cre/ERT2)Crm/Acan+, Gt(ROSA)26Sortm1Sor/Gt(ROSA)26Sor+ (more )
Note: Conditional mutant. Pregnant animals were injected i.p. with 3 mg of 4OH-tamoxifen on E14.5. Embryos were harvested 24 hours later.
Structure Level Pattern Image Note
TS23: cartilage Present 2b right Staining is observed in all developing cartilages with 4OHT treatment at E14.5.
Specimen Fig. 2c: postnatal day 2; Acantm1(cre/ERT2)Crm/Acan+, Gt(ROSA)26Sortm1Sor/Gt(ROSA)26Sor+ (more )
Note: Conditional mutant. Pregnant animals were injected i.p. with 3 mg of 4OH-tamoxifen 1 day before delivery. Pups were processed at P2.
Structure Level Pattern Image Note
TS27: cartilage Present 2c Staining is observed in all cartilage structures.
Specimen Fig. 2d: embryonic day 15.5; Acantm1(cre/ERT2)Crm/Acan+, Gt(ROSA)26Sortm1Sor/Gt(ROSA)26Sor+ (more )
Note: Conditional mutant. Pregnant animals were injected i.p. with 3 mg of 4OH-tamoxifen on E14.5. Embryos were harvested 24 hours later.
Structure Level Pattern Image Note
TS23: radius Present Regionally restricted 2d Staining is observed in proliferating and hypertrophic chondrocytes of the radius growth plate.
TS23: ulna Present Regionally restricted 2d Staining is observed in proliferating and hypertrophic chondrocytes of the ulna growth plate.
Specimen Fig. 3a: postnatal month 6; Acantm1(cre/ERT2)Crm/Acan+, Gt(ROSA)26Sortm1Sor/Gt(ROSA)26Sor+ (more )
Note: Conditional mutant. 6-month old animals were injected i.p. with 1.5 mg of 4OH-tamoxifen/ 10 g body weight for 3 consecutive days and sacrificed 3 days following final injection.
Structure Level Pattern Image Note
TS28: articular cartilage Strong 3a Staining is observed in the growth plate and articular cartilage of the tibia-femoral joint.
TS28: epiphyseal plate Strong 3a Staining is observed in the growth plate and articular cartilage of the tibia-femoral joint.
TS28: hyaline cartilage Strong 3a Staining is observed in the growth plate and articular cartilage of the tibia-femoral joint.
Specimen Fig. 3b: postnatal month 6; Acantm1(cre/ERT2)Crm/Acan+, Gt(ROSA)26Sortm1Sor/Gt(ROSA)26Sor+ (more )
Note: Conditional mutant. 6-month old animals were injected i.p. with 1.5 mg of 4OH-tamoxifen/ 10 g body weight for 3 consecutive days and sacrificed 3 days following final injection.
Structure Level Pattern Image Note
TS28: articular cartilage Strong Regionally restricted 3b Staining is observed in the growth plate of the tibia and articular cartilage of the tibial plateau.
TS28: hyaline cartilage Strong Regionally restricted 3b Staining is observed in the growth plate of the tibia and articular cartilage of the tibial plateau.
TS28: tibia Strong Regionally restricted 3b Staining is observed in the growth plate of the tibia and articular cartilage of the tibial plateau.
TS28: femur Strong Regionally restricted 3b Staining is observed in the femoral condyle.
TS28: cartilage Strong Regionally restricted 3b Staining is observed in the fibrocartilage of the meniscus of the tibia-femoral joint.
TS28: skeletal joint Strong Regionally restricted 3b Staining is observed in the fibrocartilage of the meniscus of the tibia-femoral joint.
Specimen Fig. 3c: postnatal month 6; Acantm1(cre/ERT2)Crm/Acan+, Gt(ROSA)26Sortm1Sor/Gt(ROSA)26Sor+ (more )
Note: Conditional mutant. 6-month old animals were injected i.p. with 1.5 mg of 4OH-tamoxifen/ 10 g body weight for 3 consecutive days and sacrificed 3 days following final injection.
Structure Level Pattern Image Note
TS28: hindlimb Present Regionally restricted 3c Staining is observed at the junction of the tendon with the bone in the ankle region.
Specimen Fig. 3d: postnatal month 6; Acantm1(cre/ERT2)Crm/Acan+, Gt(ROSA)26Sortm1Sor/Gt(ROSA)26Sor+ (more )
Note: Conditional mutant. 6-month old animals were injected i.p. with 1.5 mg of 4OH-tamoxifen/ 10 g body weight for 3 consecutive days and sacrificed 3 days following final injection.
Structure Level Pattern Image Note
TS28: trachea cartilage Strong 3d Staining is observed in the fibrocartilage of the trachea.
Specimen Fig. 3e: postnatal month 6; Acantm1(cre/ERT2)Crm/Acan+, Gt(ROSA)26Sortm1Sor/Gt(ROSA)26Sor+ (more )
Note: Conditional mutant. 6-month old animals were injected i.p. with 1.5 mg of 4OH-tamoxifen/ 10 g body weight for 3 consecutive days and sacrificed 3 days following final injection.
Structure Level Pattern Image Note
TS28: hyaline cartilage Strong Regionally restricted 3e staining is observed in cartilage of the ribs and spine.
TS28: rib Strong Regionally restricted 3e staining is observed in cartilage of the ribs and spine.
TS28: vertebral column Strong Regionally restricted 3e staining is observed in cartilage of the ribs and spine.
TS28: trachea cartilage Present Regionally restricted 3e
Specimen Fig. 3f: postnatal month 6; Acantm1(cre/ERT2)Crm/Acan+, Gt(ROSA)26Sortm1Sor/Gt(ROSA)26Sor+ (more )
Note: Conditional mutant. 6-month old animals were injected i.p. with 1.5 mg of 4OH-tamoxifen/ 10 g body weight for 3 consecutive days and sacrificed 3 days following final injection.
Structure Level Pattern Image Note
TS28: brain Absent 3f No staining is observed at the surface of the brain.
TS28: nose Present Regionally restricted 3f Staining is observed in the nasal cartilage.
Specimen not shown: postnatal; Acantm1(cre/ERT2)Crm/Acan+, Gt(ROSA)26Sortm1Sor/Gt(ROSA)26Sor+ (more )
Note: Conditional mutant. 6-month old animals were injected i.p. with 1.5 mg of 4OH-tamoxifen/ 10 g body weight for 3 consecutive days and sacrificed 3 days following final injection.
Structure Level Pattern Image Note
TS28: brain Absent Staining is not observed.
TS28: heart Absent Staining is not observed.
TS28: liver Absent Staining is not observed.
TS28: metanephros Absent Staining is not observed.
Specimen Fig. 4d: postnatal week 2.5; Acantm1(cre/ERT2)Crm/Acan+, Gt(ROSA)26Sortm1Sor/Gt(ROSA)26Sor+ (more )
Note: Conditional mutant. Animals were injected i.p. with 4OH-tamoxifen (3 mg/30 g) at 2 weeks of age and sacrificed at 2.5 weeks.
Structure Level Pattern Image Note
TS28: epiphyseal plate Present Regionally restricted 4d Staining is observed in most chondrocytes in the growth plate, including the lower hypertrophic zone and the deep layers of the articular cartilage.
TS28: hypertrophic cartilage zone Present Regionally restricted 4d Staining is observed in most chondrocytes in the growth plate, including the lower hypertrophic zone and the deep layers of the articular cartilage.
TS28: knee Present Regionally restricted 4d Staining is observed in most chondrocytes in the growth plate, including the lower hypertrophic zone and the deep layers of the articular cartilage.
TS28: cartilage Present 4d Staining is observed in most chondrocytes in the growth plate, including the lower hypertrophic zone and the deep layers of the articular cartilage.
Specimen Fig. 4e: postnatal week 2.5; Acantm1(cre/ERT2)Crm/Acan+, Gt(ROSA)26Sortm1Sor/Gt(ROSA)26Sor+ (more )
Note: Conditional mutant. Animals were injected i.p. with 4OH-tamoxifen (3 mg/30 g) at 2 weeks of age and sacrificed at 2.5 weeks.
Structure Level Pattern Image Note
TS28: articular cartilage Present Regionally restricted 4e Staining is observed in most chondrocytes in the growth plate, including the lower hypertrophic zone and the deep layers of the articular cartilage.
TS28: epiphyseal plate Present Regionally restricted 4e Staining is observed in most chondrocytes in the growth plate, including the lower hypertrophic zone and the deep layers of the articular cartilage.
TS28: hypertrophic cartilage zone Present Regionally restricted 4e Staining is observed in most chondrocytes in the growth plate, including the lower hypertrophic zone and the deep layers of the articular cartilage.
TS28: knee Present Regionally restricted 4e Staining is observed in most chondrocytes in the growth plate, including the lower hypertrophic zone and the deep layers of the articular cartilage.
TS28: cartilage Present 4e Staining is observed in most chondrocytes in the growth plate, including the lower hypertrophic zone and the deep layers of the articular cartilage.
Specimen Fig. 4f: postnatal week 4.5; Acantm1(cre/ERT2)Crm/Acan+, Gt(ROSA)26Sortm1Sor/Gt(ROSA)26Sor+ (more )
Note: Conditional mutant. Animals were injected i.p. with 4OH-tamoxifen (3 mg/30 g) at 4 weeks of age and sacrificed at 4.5 weeks.
Structure Level Pattern Image Note
TS28: articular cartilage Strong Regionally restricted 4f Staining is observed in chondrocytes of the growth plate and articular cartilages.
TS28: epiphyseal plate Strong Regionally restricted 4f Staining is observed in chondrocytes of the growth plate and articular cartilages.
TS28: knee Strong Regionally restricted 4f Staining is observed in chondrocytes of the growth plate and articular cartilages.
TS28: cartilage Strong 4f Staining is observed in chondrocytes of the growth plate and articular cartilages.
Specimen Fig. 4g: postnatal week 4.5; Acantm1(cre/ERT2)Crm/Acan+, Gt(ROSA)26Sortm1Sor/Gt(ROSA)26Sor+ (more )
Note: Conditional mutant. Animals were injected i.p. with 4OH-tamoxifen (3 mg/30 g) at 4 weeks of age and sacrificed at 4.5 weeks.
Structure Level Pattern Image Note
TS28: epiphyseal plate Strong Regionally restricted 4g Staining is observed in all hypertrophic chondrocytes of the growth plate.
TS28: knee Strong Regionally restricted 4g Staining is observed in all hypertrophic chondrocytes of the growth plate.
TS28: cartilage Strong 4g Staining is observed in all hypertrophic chondrocytes of the growth plate.
Specimen Fig. 4h: postnatal week 4.5; Acantm1(cre/ERT2)Crm/Acan+, Gt(ROSA)26Sortm1Sor/Gt(ROSA)26Sor+ (more )
Note: Conditional mutant. Animals were injected i.p. with 4OH-tamoxifen (3 mg/30 g) at 4 weeks of age and sacrificed at 4.5 weeks.
Structure Level Pattern Image Note
TS28: articular cartilage Present Regionally restricted 4h Staining is observed restricted in the articular cartilage to chondrocytes above the tidemark, a virtual line demarcating the superficial nonmineralized cartilage and deeper mineralized cartilage.
TS28: knee Present Regionally restricted 4h Staining is observed restricted in the articular cartilage to chondrocytes above the tidemark, a virtual line demarcating the superficial nonmineralized cartilage and deeper mineralized cartilage.
TS28: cartilage Present 4h Staining is observed restricted in the articular cartilage to chondrocytes above the tidemark, a virtual line demarcating the superficial nonmineralized cartilage and deeper mineralized cartilage.
Specimen Fig. 5b: postnatal month 6; Acantm1(cre/ERT2)Crm/Acan+, Gt(ROSA)26Sortm1Sor/Gt(ROSA)26Sor+ (more )
Note: Conditional mutant. 6-month old animals were injected i.p. with 1.5 mg of 4OH-tamoxifen/ 10 g body weight for 3 consecutive days and sacrificed 3 days following final injection.
Structure Level Pattern Image Note
TS28: epiphyseal plate Present Regionally restricted 5b Staining is restricted to chondrocytes of the growth plate.
TS28: knee Present Regionally restricted 5b Staining is restricted to chondrocytes of the growth plate.
TS28: cartilage Present 5b Staining is restricted to chondrocytes of the growth plate.
Specimen Fig. 5c: postnatal month 6; Acantm1(cre/ERT2)Crm/Acan+, Gt(ROSA)26Sortm1Sor/Gt(ROSA)26Sor+ (more )
Note: Conditional mutant. 6-month old animals were injected i.p. with 1.5 mg of 4OH-tamoxifen/ 10 g body weight for 3 consecutive days and sacrificed 3 days following final injection.
Structure Level Pattern Image Note
TS28: articular cartilage Present Regionally restricted 5c Staining is observed in chondrocytes of the articular cartilage (staining is robust in chondrocytes of uncalcified cartilage) and meniscus.
TS28: knee Present Regionally restricted 5c Staining is observed in chondrocytes of the articular cartilage (staining is robust in chondrocytes of uncalcified cartilage) and meniscus.
TS28: skeletal joint Present Regionally restricted 5c Staining is observed in chondrocytes of the articular cartilage (staining is robust in chondrocytes of uncalcified cartilage) and meniscus.
TS28: cartilage Present 5c Staining is observed in chondrocytes of the articular cartilage and meniscus restricted to the superficial layers of the fibrocartilage.
TS28: connective tissue Present Regionally restricted 5c Staining is observed in chondrocytes of the meniscus restricted to the superficial layers of the fibrocartilage.
Specimen Fig. 5d: postnatal month 6; Acantm1(cre/ERT2)Crm/Acan+, Gt(ROSA)26Sortm1Sor/Gt(ROSA)26Sor+ (more )
Note: Conditional mutant. 6-month old animals were injected i.p. with 1.5 mg of 4OH-tamoxifen/ 10 g body weight for 3 consecutive days and sacrificed 3 days following final injection.
Structure Level Pattern Image Note
TS28: articular cartilage Present Regionally restricted 5d Staining is observed in the chondrocytes of articular cartilage.
TS28: knee Present Regionally restricted 5d Staining is observed in the chondrocytes of articular cartilage.
Specimen Fig. 6a: postnatal month 6; Acantm1(cre/ERT2)Crm/Acan+, Gt(ROSA)26Sortm1Sor/Gt(ROSA)26Sor+ (more )
Note: Conditional mutant. 6-month old animals were injected i.p. with 1.5 mg of 4OH-tamoxifen/ 10 g body weight for 3 consecutive days and sacrificed 3 days following final injection.
Structure Level Pattern Image Note
TS28: vertebral column Strong 6a
Specimen Fig. 6b: postnatal month 6; Acantm1(cre/ERT2)Crm/Acan+, Gt(ROSA)26Sortm1Sor/Gt(ROSA)26Sor+ (more )
Note: Conditional mutant. 6-month old animals were injected i.p. with 1.5 mg of 4OH-tamoxifen/ 10 g body weight for 3 consecutive days and sacrificed 3 days following final injection.
Structure Level Pattern Image Note
TS28: vertebral column Strong Regionally restricted 6b Staining is observed in growth plate chondrocytes of the vertebral body and the nucleus pulposus and annulus fibrosis of the intervertebral disc.
Specimen Fig. 6c: postnatal month 6; Acantm1(cre/ERT2)Crm/Acan+, Gt(ROSA)26Sortm1Sor/Gt(ROSA)26Sor+ (more )
Note: Conditional mutant. 6-month old animals were injected i.p. with 1.5 mg of 4OH-tamoxifen/ 10 g body weight for 3 consecutive days and sacrificed 3 days following final injection.
Structure Level Pattern Image Note
TS28: vertebral column Strong Regionally restricted 6c Staining is observed in cells embedded in the fibro-cartliage rings of the annulus fibrosis and in growth plate chondrocytes of the vertebral body.
Specimen Fig. 6d: postnatal year 1; Acantm1(cre/ERT2)Crm/Acan+, Gt(ROSA)26Sortm1Sor/Gt(ROSA)26Sor+ (more )
Note: Conditional mutant. 1-year old animals were injected i.p. with 1.5 mg of 4OH-tamoxifen/ 10 g body weight for 3 consecutive days and sacrificed 1 week following final injection.
Structure Level Pattern Image Note
TS28: articular cartilage Present Regionally restricted 6d Staining is observed in growth plate cartilage, articular cartilage and meniscus similar to that observed in 6-month old animals.
TS28: epiphyseal plate Present Regionally restricted 6d Staining is observed in growth plate cartilage, articular cartilage and meniscus similar to that observed in 6-month old animals.
TS28: knee Present Regionally restricted 6d Staining is observed in growth plate cartilage, articular cartilage and meniscus similar to that observed in 6-month old animals.
TS28: skeletal joint Present Regionally restricted 6d Staining is observed in growth plate cartilage, articular cartilage and meniscus similar to that observed in 6-month old animals.
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03/25/2025
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