Acan^{tm1(cre/ERT2)Crm}
Targeted Allele Detail

Summary

• Symbol: Acan^{tm1(cre/ERT2)Crm}
• Name: aggrecan; targeted mutation 1, Benoit de Crombrugghe
• MGI ID: MGI:4420223
• Synonyms: Agc1tm(IRES-creERT2), Agc^Cre, Agc^{tm(IRES-CreERT-Neo-pgk}
• Gene: Acan Location: Chr7:78703231-78764847 bp, + strand Genetic Position: Chr7, 44.88 cM
• Alliance: Acan^{tm1(cre/ERT2)Crm} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:155982
• Parent Cell Line: G4 (ES Cell)
• Strain of Origin: (129S6/SvEvTac x C57BL/6NCrl)F1

Mutation description

• Allele Type: Targeted (Inducible, Recombinase)
• Inducer: tamoxifen
• Mutation: Insertion
• Acan^{tm1(cre/ERT2)Crm} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Acan (MGI:99602)

• Mutation details: IRES-cre/ERT2 and an FRT-flanked PGK-neo selection cassette (in reverse orientation to the target gene) was inserted into the 3' UTR of the Aggrecan gene by homologous recombination in G4 ES cells. The line was established by breeding to C57BL/6 mice. The neo cassette was not removed. (J:155982)

Generation of the Acan^{tm1(cre/ERT2)Crm} allele

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Acan (mouse) Summary of all recombinase alleles driven by Acan. Comparative matrix view of recombinase activities Alliance mouse Acan gene page

Disease models

Expression

In Mice Carrying this Mutation:

6 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Acan Mutation: 77 strains or lines available

References

• Original: J:155982 Henry SP, et al., Generation of aggrecan-CreERT2 knockin mice for inducible Cre activity in adult cartilage. Genesis. 2009 Dec;47(12):805-14
• All: 61 reference(s)