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Gene Expression Data
Assay Details
Assay
Reference: J:11388 Ott MO, et al., Early expression of the myogenic regulatory gene, myf-5, in precursor cells of skeletal muscle in the mouse embryo. Development. 1991 Apr;111(4):1097-107
Assay type: RNA in situ
MGI Accession ID: MGI:4838280
Gene symbol: Myf5
Gene name: myogenic factor 5
Probe: Myf5 probe2
Probe preparation: labelled with S35 RNA
Visualized with: Autoradiography
Results
Specimen 1A,C: embryonic day 11.0 (more )
Note: Section shows somites at the level of the hindlimbs
Structure Level Pattern Image Note
TS18: trunk somite Strong 1A/C

Specimen 2C: embryonic day 11.5 (more )
Structure Level Pattern Image Note
TS19: somite Present Regionally restricted 2C Expression in myotomes and caudal somites.
TS19: tail somite Strong 2C Stronger expression in more recently formed, somites than in mature myotomes.
TS19: trunk myotome Present 2C Stronger expression in more recently formed, somites than in mature myotomes.
TS19: mandible primordium Trace 2C Barely detectable expression in the mandibular arch.
TS19: heart Absent 2C

Specimen not shown: embryonic day 12.5 (more )
Structure Level Pattern Image Note
TS20: embryo Trace Very faint expression.

Specimen not shown: embryonic day 14.0 (more )
Structure Level Pattern Image Note
TS22: embryo Absent Expression is virtually undetectable.

Specimen 3B: embryonic day 16.0 (more )
Note: Transverse section through the abdominal region.
Structure Level Pattern Image Note
TS24: trunk Absent 3B

Specimen 4A,B: embryonic day 8.0 (more )
Note: 4 somites. Section traverses head fold and neural tube
Structure Level Pattern Image Note
TS12: head somite Present 4A/B Expression distinguishable as soon as the first somites form.

Specimen 4C,D: embryonic day 8.0 (more )
Note: Section shows neural tube flanked by two somite.
Structure Level Pattern Image Note
TS12: head somite Present Regionally restricted 4C/D Expression primarily in dorsal part, adjacent to neural tube, concentrated in myotome.

Specimen 5A,B: embryonic day 9.5 (more )
Note: 21-29 somites. Section in caudal part of embryo showing neural tube and somites.,
Structure Level Pattern Image Note
TS15: somite Present Regionally restricted 5A/B Expression in somites, mainly in dorsal region adjacent to neural tube.
TS15: dermomyotome Strong 5A/B

Specimen not shown: embryonic day 9.5 (more )
Note: Parasagittal section
Structure Level Pattern Image Note
TS15: somite Present Clear expression in both rostral and caudal somites

Specimen 6A: embryonic day 10.5 (more )
Structure Level Pattern Image Note
TS17: trunk somite Strong 6A Strong expression in rostral somites.
TS17: tail somite Present 6A Distinguishable expression in caudal somites.
TS17: hindlimb bud Absent 6A

Specimen 6C,D: embryonic day 10.5 (more )
Structure Level Pattern Image Note
TS17: trunk somite Strong 6C/D Strong expression in rostral somites.
TS17: tail somite Present 6C/D Distinguishable expression in caudal somites.
TS17: hindlimb bud Absent 6C/D

Specimen 7A,B: embryonic day 9.25 (more )
Note: 18 somites. Section in region of pharynx
Structure Level Pattern Image Note
TS14: 2nd branchial arch Present 7A/B Expression in the hyoid arch.

Specimen 7C,D: embryonic day 9.25 (more )
Note: 18 somites. Section in region of pharynx
Structure Level Pattern Image Note
TS14: 2nd branchial arch Present 7C/D Expression in the hyoid arch.

Specimen not shown: embryonic day 10.0 (more )
Note: 30-34 somites.
Structure Level Pattern Image Note
TS16: 1st branchial arch mandibular component Present Authors state that myf-5 expression precedes those of myogenin in the mandibular arch.

Specimen not shown: embryonic day 10.5 (more )
Structure Level Pattern Image Note
TS17: forelimb bud Present

Specimen not shown: embryonic day 11.0 (more )
Structure Level Pattern Image Note
TS18: forelimb bud Present

Specimen 8D,E: embryonic day 11.0 (more )
Note: Section in hindlimb region
Structure Level Pattern Image Note
TS18: hindlimb bud Present Regionally restricted 8D/E Expression in the proximal/dorsal region of the limb bud.

Specimen Table 1 E8.5: embryonic day 8.5 (more )
Note: 8-12 somites.
Structure Level Pattern Image Note
TS13: embryo Strong

Specimen Table 1 E14.5: embryonic day 14.5 (more )
Note: 64 somites.
Structure Level Pattern Image Note
TS22: embryo Ambiguous

Specimen Table 1 E17.5: embryonic day 17.5 (more )
Structure Level Pattern Image Note
TS25: embryo Absent


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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
03/25/2025
MGI 6.24
The Jackson Laboratory