Probe:	Tmed2 cDNA2
Probe preparation:	Antisense RNA
Assay notes:	Two in situ hybridization probes were described (MGI:4837916 and MGI:4837918), but the probe used in this assay was not specified. Authors indicate that both probes reported the same expression pattern and were used interchangeably.

Structure	Level	Pattern	Image	Note
TS9: ectoplacental cone	Present		7A
TS9: extraembryonic ectoderm	Present		7A

Structure	Level	Pattern	Image	Note
TS11: chorion	Present		7B

Structure	Level	Pattern	Image	Note
TS11: yolk sac	Present		7C
TS11: embryo mesoderm	Present	Regionally restricted	7C	Expression was detected in the anterior mesoderm.
TS11: primitive streak	Present	Regionally restricted	7C	Expression was detected in the proximal portion of the primitive streak.

Structure	Level	Pattern	Image	Note
TS11: chorion	Present		7D

Structure	Level	Pattern	Image	Note
TS14: central nervous system	Strong	Regionally restricted	7E	Expression was high in the neural epithelium.
TS14: primitive streak	Strong	Regionally restricted	7E	Expression was high in the peri-nodal region.
TS14: allantois	Strong		7E
TS14: tail bud	Strong		7E
TS14: trunk unsegmented mesenchyme	Strong		7E
TS14: trunk mesenchyme	Weak		7E	Expression was low in the lateral plate.
TS14: somite	Weak		7E
TS14: embryo	Present	Regionally restricted	7E	Expression was not found in the midline.

Structure	Level	Pattern	Image	Note
TS14: prosencephalon	Present	Regionally restricted	7F	Expression was highest in the forebrain.
TS14: branchial arch	Present		7F
TS14: tail bud	Present		7F
TS14: heart	Absent		7F

Structure	Level	Pattern	Image	Note
TS15: forelimb bud	Present		7G left
TS15: future forebrain	Present		7G left
TS15: neural tube	Present		7G left
TS15: otocyst	Present		7G left

Structure	Level	Pattern	Image	Note
TS15: branchial arch	Present		7G right
TS15: forelimb bud	Present		7G right
TS15: hepatic primordium	Present		7G right
TS15: optic vesicle	Present		7G right
TS15: otocyst	Present		7G right
TS15: tail	Present		7G right

Structure	Level	Pattern	Image	Note
TS7: epiblast	Present	Ubiquitous	S1A
TS7: extraembryonic ectoderm	Present	Ubiquitous	S1A

Structure	Level	Pattern	Image	Note
TS7: epiblast	Present	Widespread	S1B	Expression was equally distributed throughout the extraembryonic and embryonic ectoderms.
TS7: extraembryonic ectoderm	Present	Widespread	S1B	Expression was equally distributed throughout the extraembryonic and embryonic ectoderms.
TS7: extraembryonic visceral endoderm	Strong	Regionally restricted	S1B	Expression was found at higher levels in the visceral and distal visceral endoderm compared to the embryonic and extraembryonic ectoderm.
TS7: parietal endoderm	Present		S1B

Structure	Level	Pattern	Image	Note
TS7: ectoplacental cone	Present		S1C	Expression was higher in the ectoplacental cone and visceral endoderm compared to epiblast.
TS7: epiblast	Present		S1C	Expression was higher in the ectoplacental cone and visceral endoderm compared to epiblast.
TS7: extraembryonic visceral endoderm	Present		S1C	Expression was higher in the ectoplacental cone and visceral endoderm compared to epiblast.

Structure	Level	Pattern	Image	Note
TS7: epiblast	Strong		S1D

Structure	Level	Pattern	Image	Note
TS9: ectoplacental cone	Strong		S1E

Structure	Level	Pattern	Image	Note
TS9: embryo ectoderm	Weak	Widespread	S1F	Low expression levels were detected throughout the embryonic ectoderm.

Structure	Level	Pattern	Image	Note
TS9: embryo	Present	Regionally restricted	S1G	Expression was detected in the posterior region of the embryo.
TS9: embryo ectoderm	Present	Scattered	S1G
TS9: extraembryonic visceral endoderm	Present	Scattered	S1G

Structure	Level	Pattern	Image	Note
TS9: embryo	Present	Regionally restricted	S1H	Expression was detected in the posterior region of the embryo.
TS9: embryo ectoderm	Present	Scattered	S1H
TS9: extraembryonic visceral endoderm	Present	Scattered	S1H

Structure	Level	Pattern	Image	Note
TS11: amnion	Present		S1K , S1I , S1L
TS11: embryo mesoderm	Present	Regionally restricted	S1I , S1L , S1K	Expression was detected in the anterior mesoderm with lower levels detected in posterior mesoderm.
TS11: embryo ectoderm	Present		S1L , S1I

Structure	Level	Pattern	Image	Note
TS11: ectoplacental cone	Present		S1J

Structure	Level	Pattern	Image	Note
TS13: embryo	Present	Widespread	S1M
TS13: foregut diverticulum	Present		S1M

Structure	Level	Pattern	Image	Note
TS13: dorsal aorta	Present		S1N
TS13: somite	Present		S1N
TS13: neural tube	Absent		S1N	Expression was not detected in closed neural tube.
TS13: midgut endoderm	Absent		S1N

Structure	Level	Pattern	Image	Note
TS13: lateral plate mesoderm	Present		S1O
TS13: embryo endoderm	Absent		S1O

Structure	Level	Pattern	Image	Note
TS14: tail bud	Strong	Regionally restricted	S1P	Expression levels were high in the posterior tailbud mesoderm.
TS14: hindgut diverticulum	Absent		S1P

Structure	Level	Pattern	Image	Note
TS17: embryo	Present	Widespread		Broad expression was detected in most embryonic structures.
TS17: primitive ventricle	Present

Structure	Level	Pattern	Image	Note
TS13: chorionic plate	Present		8A
TS13: allantois	Present		8A
TS13: trophoblast giant cell	Present		8A

Structure	Level	Pattern	Image	Note
TS28: placenta	Present	Widespread	8B	Expression was detected throughout the placenta with the exception of the allantois.
TS15: labyrinthine zone	Present		8B
TS15: spongiotrophoblast	Present		8B
TS15: trophoblast giant cell	Present		8B
TS15: allantois	Absent		8B

Structure	Level	Pattern	Image	Note
TS17: labyrinthine zone	Present		8D , 8C
TS17: spongiotrophoblast	Present		8C
TS17: trophoblast giant cell	Present	Regionally restricted	8C	Expression was detected in a subset of giant cells.
TS28: decidua	Present	Regionally restricted	8C	Expression was detected in a subset of cells in the maternal decidua.
TS17: blood vessel	Present	Regionally restricted	8C	Expression was detected in fetal blood vessels in the placenta.