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Gene Expression Data
Assay Details
Assay
Reference: J:90391 Yu K, et al., Conditional inactivation of FGF receptor 2 reveals an essential role for FGF signaling in the regulation of osteoblast function and bone growth. Development. 2003 Jul;130(13):3063-74
Assay type: Recombinase reporter
MGI Accession ID: MGI:4830608
Gene symbol: Twist2
Gene name: twist basic helix-loop-helix transcription factor 2
Reporter: lacZ
Direct Detection
Results
Specimen Fig. 1D: embryonic day 11.5; Gt(ROSA)26Sortm1Sor/Gt(ROSA)26Sor+, Twist2tm1.1(cre)Dor/Twist2+ (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS19: branchial arch Present 1D
TS19: somite Present 1D
Specimen Fig. 1E: embryonic day 15.5; Gt(ROSA)26Sortm1Sor/Gt(ROSA)26Sor+, Twist2tm1.1(cre)Dor/Twist2+ (more )
Note: Conditional mutant. This is a sagittal section through the embryo.
Structure Level Pattern Image Note
TS23: embryo Present Regionally restricted 1E Staining is not observed in ectodermal tissues.
Specimen Fig. 1F: embryonic day 11.5; Gt(ROSA)26Sortm1Sor/Gt(ROSA)26Sor+, Twist2tm1.1(cre)Dor/Twist2+ (more )
Note: Conditional mutant. This is a paraffin section of a limb bud.
Structure Level Pattern Image Note
TS19: forelimb bud mesenchyme Present 1F Mesenchymal condensations in the forelimb bud exhibit staining.
TS19: forelimb bud ectoderm Absent 1F Ectodermal epithelium of the forelimb bud does not stain.
Specimen Fig. 1G: embryonic day 16.5; Gt(ROSA)26Sortm1Sor/Gt(ROSA)26Sor+, Twist2tm1.1(cre)Dor/Twist2+ (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS24: femur Present 1G Perichondrium and periosteum are uniformly stained in the femur, but there are some unlabeled chondrocyte apparent.
Specimen Fig. 1I: postnatal adult; Gt(ROSA)26Sortm1Sor/Gt(ROSA)26Sor+, Twist2tm1.1(cre)Dor/Twist2+ (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS28: tibia Present Regionally restricted 1I Staining is observed in chondrocytes and osteoblasts in the growth plate of the tibia.
Specimen Fig. 1J: postnatal adult; Gt(ROSA)26Sortm1Sor/Gt(ROSA)26Sor+, Twist2tm1.1(cre)Dor/Twist2+ (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS28: tibia Present Regionally restricted 1J Staining is observed in the cortical bone. Staining is observed in the chondrocytes in growth plate cartilage. There are occasional clones of chondrocytes that fail to stain.
TS28: perichondrium Present 1J Staining is observed in the osteoblasts of the perichondrium, periosteum and endosteum.
TS28: periosteum Present 1J Staining is observed in the osteoblasts of the perichondrium, periosteum and endosteum.
Specimen Fig. 1L: postnatal adult; Gt(ROSA)26Sortm1Sor/Gt(ROSA)26Sor+, Twist2tm1.1(cre)Dor/Twist2+ (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS28: bone marrow Absent 1L
TS28: hindlimb musculature Absent 1L
TS28: bone Present Regionally restricted 1L Staining is observed in osteoblasts lining the bone trabeculae. There is no staining in the osteoclasts.
TS28: tibia Present Uniform 1L Perichondrium and periosteum are uniformly stained.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/18/2025
MGI 6.24 		The Jackson Laboratory