Probe:	Amer3 cDNA1
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS19: hindbrain	Present		1C C3
TS19: midbrain	Present		1C C3
TS19: neural tube	Present		1C C3
TS19: telencephalon	Present		1C C3
TS19: inner ear	Present	Regionally restricted	1C C3	otic vesicle

Structure	Level	Pattern	Image	Note
TS19: neural tube	Present		1C C3'
TS19: inner ear	Present	Regionally restricted	1C C3'	otic vesicle

Structure	Level	Pattern	Image	Note
TS17: acoustic ganglion	Present		3A
TS17: facial ganglion	Present		3A
TS17: glossopharyngeal ganglion	Present		3A
TS17: hindbrain marginal layer	Present		3A
TS17: trigeminal ganglion	Present		3A
TS17: vagus ganglion	Present		3A
TS17: neural tube lateral wall	Present	Regionally restricted	3A	Expression is in the marginal layer, absent in the ventricular zone.

Structure	Level	Pattern	Image	Note
TS17: hindbrain marginal layer	Present		3a
TS17: trigeminal ganglion	Present		3a
TS17: neural tube lateral wall	Present	Regionally restricted	3a	Expression is in the marginal layer, absent in the ventricular zone.

Structure	Level	Pattern	Image	Note
TS17: neural tube lateral wall	Present	Regionally restricted	3b	Expression is in the marginal layer with the strongest signals in presumptive motor neuron pools (postmitotic neurons) of the basal plate, absent in the ventricular zone.
TS17: dorsal root ganglion	Present		3b

Structure	Level	Pattern	Image	Note
TS17: neural tube	Present	Regionally restricted	3c	Expression is in the marginal layer, absent in the ventricular zone.
TS17: sclerotome	Present	Regionally restricted	3c	Expression is restricted to the developing somites of the medial part of the body.

Structure	Level	Pattern	Image	Note
TS17: neural tube lateral wall	Present	Regionally restricted	3c'	Expression is in the marginal layer, absent in the ventricular zone.

Structure	Level	Pattern	Image	Note
TS17: neural tube	Present		3B	Expression is in the marginal layer, absent in the ventricular zone.

Structure	Level	Pattern	Image	Note
TS17: brain	Present		3C
TS17: trigeminal ganglion	Present		3C

Structure	Level	Pattern	Image	Note
TS17: brain	Present		3D

Structure	Level	Pattern	Image	Note
TS17: forebrain	Present	Regionally restricted	3d	Expression is antero-lateral.
TS17: midbrain	Present	Scattered	3d	Expression is ventral.

Structure	Level	Pattern	Image	Note
TS17: neural tube lateral wall	Present		3e	Expression is in the marginal layer with the strongest signals in presumptive motor neuron pools (postmitotic neurons) of the basal plate, absent in the ventricular zone.

Structure	Level	Pattern	Image	Note
TS17: neural tube	Present		3f	Expression is in the marginal layer with the strongest signals in presumptive motor neuron pools (postmitotic neurons) of the basal plate, absent in the ventricular zone.

Structure	Level	Pattern	Image	Note
TS19: olfactory epithelium	Present		4A Amer3
TS19: heart	Absent		4A Amer3

Structure	Level	Pattern	Image	Note
TS20: body wall mesenchyme	Present	Regionally restricted	4B Amer3	Expression is in differentiating muscle.
TS20: limb mesenchyme	Present	Regionally restricted	4B Amer3	Expression is in differentiating muscle.
TS20: medulla oblongata	Present		4B Amer3
TS20: spinal cord	Present		4B Amer3
TS20: telencephalon	Present		4B Amer3
TS20: heart	Absent		4B Amer3

Structure	Level	Pattern	Image	Note
TS21: dorsal root ganglion	Present		4C Amer3
TS21: medulla oblongata	Present		4C Amer3
TS21: spinal cord	Present		4C Amer3
TS21: telencephalon	Present		4C Amer3
TS21: heart	Absent		4C Amer3

Structure	Level	Pattern	Image	Note
TS22: inferior cervical ganglion	Present		4D Amer3
TS22: medulla oblongata	Present		4D Amer3
TS22: midbrain	Present		4D Amer3
TS22: pons	Present		4D Amer3
TS22: spinal cord	Present		4D Amer3
TS22: telencephalon	Present		4D Amer3
TS22: thalamus	Present		4D Amer3
TS22: vestibulo-cochlear ganglion	Present		4D Amer3
TS22: cortical plate	Present		4D Amer3
TS22: heart	Absent		4D Amer3

Structure	Level	Pattern	Image	Note
TS23: dorsal grey horn	Present		5A Amer3
TS23: dorsal root ganglion	Present		5A Amer3
TS23: ventral grey horn	Present		5A Amer3
TS23: spinal cord marginal layer	Absent		5A Amer3

Structure	Level	Pattern	Image	Note
TS22: dorsal root ganglion	Present		5B Amer3

Structure	Level	Pattern	Image	Note
TS22: inferior cervical ganglion	Present		5C Amer3

Structure	Level	Pattern	Image	Note
TS22: trigeminal ganglion	Present		5D Amer3

Structure	Level	Pattern	Image	Note
TS21: cortical plate	Present		6A Amer3
TS21: forebrain	Present		6A Amer3
TS21: cerebral cortex ventricular layer	Absent		6A Amer3

Structure	Level	Pattern	Image	Note
TS21: 4th ventricle choroid plexus	Present		6B Amer3
TS21: medulla oblongata part of 4th ventricle choroid plexus	Present		6B Amer3

Structure	Level	Pattern	Image	Note
TS22: cochlear duct epithelium	Present		6C Amer3

Structure	Level	Pattern	Image	Note
TS21: neural retinal epithelium	Present		6D Amer3

Structure	Level	Pattern	Image	Note
TS26: retina inner nuclear layer	Present		6E Amer3

Structure	Level	Pattern	Image	Note
TS23: olfactory epithelium	Present		7A Amer3

Structure	Level	Pattern	Image	Note
TS26: olfactory epithelium	Present		7B Amer3

Structure	Level	Pattern	Image	Note
TS23: vibrissa	Present		7C Amer3

Structure	Level	Pattern	Image	Note
TS23: submandibular gland primordium	Present	Regionally restricted	7D Amer3	Expression is in the presumptive submandibular ganglia along the ducts of the submandibular gland.

Structure	Level	Pattern	Image	Note
TS20: lung	Absent		7E Amer3

Structure	Level	Pattern	Image	Note
TS23: tongue	Present	Single cells	7F Amer3	Expression is in clusters of cells at the ventrolateral portion of the tongue, absent elsewhere.

Structure	Level	Pattern	Image	Note
TS22: hypogastric plexus	Present		9l

Structure	Level	Pattern	Image	Note
TS14: embryo	Absent		S1B Amer3

Structure	Level	Pattern	Image	Note
TS15: facio-acoustic preganglion complex	Very strong		S1C left
TS15: glossopharyngeal preganglion	Very strong		S1C left
TS15: glossopharyngeal-vagus preganglion complex	Very strong		S1C left
TS15: vagus preganglion	Very strong		S1C left
TS15: neural tube	Present	Regionally restricted	S1C left	Expression is in the anterior part of the neural tube.

Structure	Level	Pattern	Image	Note
TS15: cranial preganglion	Present		S1C a

Structure	Level	Pattern	Image	Note
TS15: neural tube	Present		S1C b
TS15: trunk sclerotome	Present	Regionally restricted	S1C b	Expression is in the developing somites of the medial part of the body.

Structure	Level	Pattern	Image	Note
TS11: embryo	Absent

Structure	Level	Pattern	Image	Note
TS13: embryo	Absent

Structure	Level	Pattern	Image	Note
TS21: thymus primordium	Absent

Structure	Level	Pattern	Image	Note
TS22: thymus primordium	Absent